Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   FIR93_RS03715 Genome accession   NZ_CP041031
Coordinates   771508..772167 (-) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli strain PT109     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 766508..777167
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FIR93_RS03690 (FIR93_03690) ygiN 767409..767723 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  FIR93_RS03695 (FIR93_03695) mdaB 767754..768335 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  FIR93_RS24545 - 768585..769064 (+) 480 WP_000065332.1 Hcp family type VI secretion system effector -
  FIR93_RS24550 - 769067..769777 (+) 711 WP_000834030.1 hypothetical protein -
  FIR93_RS03705 (FIR93_03705) ygiZ 769784..770116 (+) 333 WP_000914690.1 DUF2645 family protein -
  FIR93_RS03710 (FIR93_03710) qseC 770162..771511 (-) 1350 WP_000673358.1 quorum sensing histidine kinase QseC -
  FIR93_RS03715 (FIR93_03715) ciaR 771508..772167 (-) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  FIR93_RS03720 (FIR93_03720) ygiW 772319..772711 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  FIR93_RS03725 (FIR93_03725) ygiV 772764..773246 (+) 483 WP_000183505.1 GyrI-like domain-containing protein -
  FIR93_RS03730 (FIR93_03730) mqsR 773451..773747 (+) 297 WP_000415585.1 type II toxin-antitoxin system toxin MqsR -
  FIR93_RS03735 (FIR93_03735) mqsA 773749..774144 (+) 396 WP_000650107.1 type II toxin-antitoxin system antitoxin MqsA -
  FIR93_RS03740 (FIR93_03740) ygiS 774277..775884 (+) 1608 WP_001375094.1 ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=324487 FIR93_RS03715 WP_001221502.1 771508..772167(-) (ciaR) [Escherichia coli strain PT109]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=324487 FIR93_RS03715 WP_001221502.1 771508..772167(-) (ciaR) [Escherichia coli strain PT109]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365