Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   FIU18_RS00235 Genome accession   NZ_CP041008
Coordinates   45566..46189 (+) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain FDAARGOS_767     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 40566..51189
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FIU18_RS00220 (FIU18_00220) - 41256..43724 (-) 2469 WP_003114576.1 penicillin-binding protein 1A -
  FIU18_RS00225 (FIU18_00225) pilM 43909..44973 (+) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  FIU18_RS00230 (FIU18_00230) pilN 44973..45569 (+) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  FIU18_RS00235 (FIU18_00235) pilO 45566..46189 (+) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  FIU18_RS00240 (FIU18_00240) comL/pilP 46186..46710 (+) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  FIU18_RS00245 (FIU18_00245) pilQ 46764..48908 (+) 2145 WP_003114575.1 type 4a pilus secretin PilQ Machinery gene
  FIU18_RS00250 (FIU18_00250) aroK 48920..49438 (+) 519 WP_003095833.1 shikimate kinase AroK -
  FIU18_RS00255 (FIU18_00255) aroB 49488..50594 (+) 1107 WP_003114574.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=324137 FIU18_RS00235 WP_003103268.1 45566..46189(+) (pilO) [Pseudomonas aeruginosa strain FDAARGOS_767]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=324137 FIU18_RS00235 WP_003103268.1 45566..46189(+) (pilO) [Pseudomonas aeruginosa strain FDAARGOS_767]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGTGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTTGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACTTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401