Detailed information NTG0321675

Bioinformatically predicted

Overview

• Name: ciaR
• Type: Regulator
• Locus tag: FEV15_RS22560
• Genome accession: NZ_CP040397
• Coordinates: 4627513..4628172 (+)
• Length: 219 a.a.
• NCBI ID: WP_001221502.1
• Uniprot ID: B7LGG8
• Organism: Escherichia coli strain BA22372
• Function: repress competence development; post-transcriptional repression of CSP production (predicted from homology) Competence regulation

Genomic Context

Location: 4622513..4633172

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FEV15_RS22535 (FEV15_22535)	ygiS	4623796..4625403 (-)	1608	WP_001375094.1	ABC transporter substrate-binding protein	-
FEV15_RS22540 (FEV15_22540)	mqsA	4625536..4625931 (-)	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	-
FEV15_RS22545 (FEV15_22545)	mqsR	4625933..4626229 (-)	297	WP_000415585.1	type II toxin-antitoxin system toxin MqsR	-
FEV15_RS22550 (FEV15_22550)	ygiV	4626434..4626916 (-)	483	WP_000183505.1	GyrI-like domain-containing protein	-
FEV15_RS22555 (FEV15_22555)	ygiW	4626969..4627361 (-)	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
FEV15_RS22560 (FEV15_22560)	ciaR	4627513..4628172 (+)	660	WP_001221502.1	quorum sensing response regulator transcription factor QseB	Regulator
FEV15_RS22565 (FEV15_22565)	qseC	4628169..4629518 (+)	1350	WP_000673358.1	quorum sensing histidine kinase QseC	-
FEV15_RS22570 (FEV15_22570)	ygiZ	4629564..4629896 (-)	333	WP_000914690.1	DUF2645 family protein	-
FEV15_RS24735	-	4629903..4630613 (-)	711	WP_000834030.1	hypothetical protein	-
FEV15_RS24740	-	4630616..4631095 (-)	480	WP_000065332.1	Hcp family type VI secretion system effector	-
FEV15_RS22580 (FEV15_22580)	mdaB	4631345..4631926 (+)	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
FEV15_RS22585 (FEV15_22585)	ygiN	4631957..4632271 (+)	315	WP_000958598.1	putative quinol monooxygenase	-

Sequence

Protein

• Length: 219 a.a.
• Molecular weight: 24745.66 Da
• Isoelectric Point: 6.6543

>NTDB_id=321675 FEV15_RS22560 WP_001221502.1 4627513..4628172(+) (ciaR) [Escherichia coli strain BA22372]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide

• Length: 660 bp

>NTDB_id=321675 FEV15_RS22560 WP_001221502.1 4627513..4628172(+) (ciaR) [Escherichia coli strain BA22372]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

• Transcriptional regulatory protein, C terminal (Trans_reg_C) (145-216)

• Response regulator receiver domain (Response_reg) (3-112)

3D structure

• AlphaFold DB: B7LGG8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ciaR	Streptococcus pneumoniae Rx1	38.326	100	0.397
ciaR	Streptococcus pneumoniae D39	38.326	100	0.397
ciaR	Streptococcus pneumoniae R6	38.326	100	0.397
ciaR	Streptococcus pneumoniae TIGR4	38.326	100	0.397
ciaR	Streptococcus mutans UA159	35.874	100	0.365