Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   PCC8801_RS20385 Genome accession   NC_011726
Coordinates   4376697..4377806 (-) Length   369 a.a.
NCBI ID   WP_015785152.1    Uniprot ID   B7K650
Organism   Rippkaea orientalis PCC 8801     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4371697..4382806
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCC8801_RS20370 (PCC8801_4170) - 4372824..4375055 (-) 2232 WP_015957307.1 secretin and TonB N-terminal domain-containing protein -
  PCC8801_RS20375 (PCC8801_4171) - 4375142..4375975 (-) 834 WP_015957308.1 hypothetical protein -
  PCC8801_RS20380 (PCC8801_4172) - 4375972..4376697 (-) 726 WP_015785151.1 PilN domain-containing protein -
  PCC8801_RS20385 (PCC8801_4173) pilM 4376697..4377806 (-) 1110 WP_015785152.1 type IV pilus assembly protein PilM Machinery gene
  PCC8801_RS20390 (PCC8801_4174) - 4378009..4379289 (+) 1281 WP_015785153.1 ABC transporter substrate-binding protein -
  PCC8801_RS20395 (PCC8801_4175) - 4379386..4380864 (+) 1479 WP_015957309.1 serine/threonine protein kinase -
  PCC8801_RS20400 (PCC8801_4176) - 4381355..4381570 (+) 216 WP_015785155.1 hypothetical protein -
  PCC8801_RS20405 (PCC8801_4177) - 4381828..4382178 (+) 351 WP_015785156.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41118.42 Da        Isoelectric Point: 4.4231

>NTDB_id=32126 PCC8801_RS20385 WP_015785152.1 4376697..4377806(-) (pilM) [Rippkaea orientalis PCC 8801]
MLNRLKALLAGKSPGVGLEITPERLNLAQLSKHKQGYKLTKFCTTEIPEGIFEEGKILDSPRLAELIQEMLKENKINTKR
VATAVPMREAIIRVIPIPAELDDEELRDMVLNTEAALYLPYPREEVDLDYQKLGFFEDEDGIEKVQVLLVATRKEVTDSY
MDTFQQAGLEVDVLEINSFALIRTIREQLRQFSSKEAAVLVDVEFDNTEIAIVVDGVPQFSRTVPIGTYQMQNALSRAMN
LPPSRNPEILQGMTIPVTQFDSLSTQGTAINPGMTALMRVLGELTDELRRSINFYLNQSEELEVVQMLLAGPGGGLVQLD
EYLTHRLSIPTMQVDPVTTLALELDKDIPAVQRPGLGTVLGLGLREVKA

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=32126 PCC8801_RS20385 WP_015785152.1 4376697..4377806(-) (pilM) [Rippkaea orientalis PCC 8801]
ATGTTAAATCGGTTAAAAGCTTTATTAGCAGGGAAATCCCCAGGGGTTGGCTTAGAAATCACCCCCGAAAGACTGAATTT
AGCCCAATTGTCCAAACACAAGCAAGGCTATAAATTGACCAAGTTCTGCACCACAGAAATTCCCGAAGGGATCTTTGAGG
AGGGAAAAATTCTCGATTCTCCCAGATTAGCGGAACTGATCCAAGAGATGCTCAAAGAGAATAAAATTAACACCAAACGG
GTAGCGACGGCTGTTCCCATGCGCGAAGCCATTATTCGGGTGATTCCCATTCCGGCTGAACTCGATGACGAGGAATTGCG
CGACATGGTGTTAAATACGGAAGCGGCCTTGTATTTGCCCTATCCGAGAGAAGAAGTCGATCTCGACTATCAGAAGCTAG
GCTTCTTTGAAGACGAAGACGGCATCGAAAAAGTTCAAGTCCTCCTAGTAGCCACCCGTAAAGAGGTAACAGACTCCTAT
ATGGACACCTTTCAGCAAGCGGGACTGGAAGTGGATGTGCTTGAAATTAACAGTTTTGCCTTAATACGGACAATTAGGGA
ACAATTGAGGCAATTTAGTTCAAAAGAAGCCGCCGTCTTAGTCGATGTGGAGTTTGACAATACGGAAATTGCGATCGTCG
TCGATGGGGTTCCTCAATTTTCACGGACGGTTCCCATTGGAACCTACCAGATGCAAAATGCCCTATCACGGGCGATGAAT
TTACCCCCCTCGCGGAACCCAGAAATTTTGCAGGGGATGACCATTCCCGTGACTCAGTTTGACAGCTTGAGTACCCAAGG
AACCGCGATCAACCCAGGGATGACTGCCCTGATGAGAGTCTTAGGAGAACTCACCGACGAATTGAGGCGATCAATCAATT
TTTATTTAAACCAAAGTGAAGAGTTGGAGGTCGTACAAATGTTGTTAGCAGGTCCTGGAGGAGGACTGGTACAACTCGAT
GAATATTTGACCCATCGCTTAAGTATTCCTACCATGCAAGTTGATCCAGTAACCACCTTAGCCTTAGAACTTGACAAAGA
CATTCCGGCAGTTCAACGGCCAGGGTTAGGAACAGTATTAGGGTTAGGGTTACGGGAGGTTAAAGCCTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(17-241)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7K650

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Synechocystis sp. PCC 6803

80.328

99.187

0.797

Multiple sequence alignment