Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   D9C16_RS01025 Genome accession   NZ_CP032861
Coordinates   169168..169551 (+) Length   127 a.a.
NCBI ID   WP_014480254.1    Uniprot ID   -
Organism   Bacillus subtilis subsp. subtilis strain N1-1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 164168..174551
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D9C16_RS00990 (D9C16_00990) corA 164622..165575 (+) 954 WP_014480260.1 magnesium transporter CorA -
  D9C16_RS00995 (D9C16_00995) - 165577..165774 (+) 198 WP_014480259.1 CBS domain-containing protein -
  D9C16_RS01000 (D9C16_01000) comGA 165986..167056 (+) 1071 WP_004399124.1 competence protein ComGA Machinery gene
  D9C16_RS01005 (D9C16_01005) comGB 167043..168080 (+) 1038 WP_031600685.1 comG operon protein ComGB Machinery gene
  D9C16_RS01010 (D9C16_01010) comGC 168094..168390 (+) 297 WP_003230162.1 comG operon protein ComGC Machinery gene
  D9C16_RS01015 (D9C16_01015) comGD 168380..168811 (+) 432 WP_014480256.1 comG operon protein ComGD Machinery gene
  D9C16_RS01020 (D9C16_01020) comGE 168795..169142 (+) 348 WP_014480255.1 ComG operon protein 5 Machinery gene
  D9C16_RS01025 (D9C16_01025) comGF 169168..169551 (+) 384 WP_014480254.1 ComG operon protein ComGF Machinery gene
  D9C16_RS01030 (D9C16_01030) comGG 169552..169926 (+) 375 WP_014480253.1 ComG operon protein ComGG Machinery gene
  D9C16_RS01035 (D9C16_01035) spoIITA 169997..170176 (+) 180 WP_014480252.1 YqzE family protein -
  D9C16_RS01040 (D9C16_01040) yqzG 170218..170544 (-) 327 WP_003246051.1 YqzG/YhdC family protein -
  D9C16_RS01045 (D9C16_01045) tapA 170816..171577 (+) 762 WP_014480251.1 amyloid fiber anchoring/assembly protein TapA -
  D9C16_RS01050 (D9C16_01050) sipW 171561..172133 (+) 573 WP_003230181.1 signal peptidase I SipW -
  D9C16_RS01055 (D9C16_01055) tasA 172197..172982 (+) 786 WP_014480250.1 biofilm matrix protein TasA -
  D9C16_RS01060 (D9C16_01060) sinR 173075..173410 (-) 336 WP_003226345.1 transcriptional regulator SinR Regulator
  D9C16_RS01065 (D9C16_01065) sinI 173444..173617 (-) 174 WP_003230187.1 anti-repressor SinI Regulator

Sequence

Protein


Download         Length: 127 a.a.        Molecular weight: 14329.42 Da        Isoelectric Point: 5.8949

>NTDB_id=319541 D9C16_RS01025 WP_014480254.1 169168..169551(+) (comGF) [Bacillus subtilis subsp. subtilis strain N1-1]
MLISGSLAAIFHLFLSRQQEHDGFTQQEWMISIEQMMNECKESQAVKTAEHGSVLICTNLSGQDIRFEIYHSMIRKRVDG
KGHVPILDHITAMKADIENGVVLLKIESEDQKVYQTAFPVYSYLGGG

Nucleotide


Download         Length: 384 bp        

>NTDB_id=319541 D9C16_RS01025 WP_014480254.1 169168..169551(+) (comGF) [Bacillus subtilis subsp. subtilis strain N1-1]
TTGCTCATATCAGGATCGTTAGCTGCGATTTTCCATCTGTTTTTGTCTCGACAGCAGGAACATGACGGTTTCACACAGCA
AGAATGGATGATTTCGATAGAACAGATGATGAATGAATGCAAGGAGTCACAGGCAGTTAAGACAGCCGAGCATGGGAGCG
TGTTAATCTGCACCAATCTTTCCGGGCAGGACATCCGTTTCGAAATTTATCATTCAATGATAAGAAAAAGAGTGGATGGC
AAAGGGCATGTTCCGATTTTAGATCATATTACTGCCATGAAAGCTGATATTGAAAATGGTGTTGTTTTGCTGAAAATTGA
GAGTGAGGACCAAAAAGTGTATCAAACTGCTTTTCCAGTCTATTCGTATTTAGGAGGGGGGTGA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(16-114)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Bacillus subtilis subsp. subtilis str. 168

98.425

100

0.984

Multiple sequence alignment