Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   D9C16_RS00550 Genome accession   NZ_CP032861
Coordinates   85971..86588 (+) Length   205 a.a.
NCBI ID   WP_014480312.1    Uniprot ID   -
Organism   Bacillus subtilis subsp. subtilis strain N1-1     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 80971..91588
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D9C16_RS00515 (D9C16_00515) aroE 81629..82471 (+) 843 WP_014480317.1 shikimate dehydrogenase -
  D9C16_RS00520 (D9C16_00520) yhbY 82465..82755 (+) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  D9C16_RS00525 (D9C16_00525) nadD 82767..83336 (+) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  D9C16_RS00530 (D9C16_00530) yqeK 83326..83886 (+) 561 WP_014480316.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  D9C16_RS00535 (D9C16_00535) rsfS 83904..84260 (+) 357 WP_014480315.1 ribosome silencing factor -
  D9C16_RS00540 (D9C16_00540) yqeM 84257..85000 (+) 744 WP_014480314.1 class I SAM-dependent methyltransferase -
  D9C16_RS00545 (D9C16_00545) comER 85066..85887 (-) 822 WP_014480313.1 late competence protein ComER -
  D9C16_RS00550 (D9C16_00550) comEA 85971..86588 (+) 618 WP_014480312.1 competence protein ComEA Machinery gene
  D9C16_RS00555 (D9C16_00555) comEB 86655..87224 (+) 570 WP_003229978.1 ComE operon protein 2 -
  D9C16_RS00560 (D9C16_00560) comEC 87228..89558 (+) 2331 WP_033881047.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  D9C16_RS00565 (D9C16_00565) yqzM 89598..89732 (-) 135 WP_003229983.1 YqzM family protein -
  D9C16_RS00570 (D9C16_00570) - 89773..89922 (+) 150 WP_003229985.1 hypothetical protein -
  D9C16_RS00575 (D9C16_00575) holA 89962..91005 (+) 1044 WP_014480310.1 DNA polymerase III subunit delta -
  D9C16_RS00580 (D9C16_00580) rpsT 91020..91286 (-) 267 WP_003229989.1 30S ribosomal protein S20 -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21781.51 Da        Isoelectric Point: 4.7220

>NTDB_id=319532 D9C16_RS00550 WP_014480312.1 85971..86588(+) (comEA) [Bacillus subtilis subsp. subtilis strain N1-1]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEEIAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=319532 D9C16_RS00550 WP_014480312.1 85971..86588(+) (comEA) [Bacillus subtilis subsp. subtilis strain N1-1]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAATAGCAGTGCAGCAAGGTGGCGGAGGGTCAG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

99.512

100

0.995
  comEA Staphylococcus aureus MW2

37.273

100

0.4
  comEA Staphylococcus aureus N315

36.364

100

0.39

Multiple sequence alignment