Detailed information NTG0319528

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: D9C14_RS21915
• Genome accession: NZ_CP032860
• Coordinates: 4163365..4163982 (-)
• Length: 205 a.a.
• NCBI ID: WP_004398514.1
• Uniprot ID: A0AAE2SLR2
• Organism: Bacillus subtilis subsp. subtilis strain SSJ-1
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4158365..4168982

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
D9C14_RS21885 (D9C14_21885)	rpsT	4158667..4158933 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
D9C14_RS21890 (D9C14_21890)	holA	4158948..4159991 (-)	1044	WP_003229987.1	DNA polymerase III subunit delta	-
D9C14_RS21895 (D9C14_21895)	-	4160031..4160180 (-)	150	WP_003229985.1	hypothetical protein	-
D9C14_RS21900 (D9C14_21900)	yqzM	4160221..4160355 (+)	135	WP_003229983.1	YqzM family protein	-
D9C14_RS21905 (D9C14_21905)	comEC	4160395..4162725 (-)	2331	WP_009967776.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
D9C14_RS21910 (D9C14_21910)	comEB	4162729..4163298 (-)	570	WP_003229978.1	ComE operon protein 2	-
D9C14_RS21915 (D9C14_21915)	comEA	4163365..4163982 (-)	618	WP_004398514.1	competence protein ComEA	Machinery gene
D9C14_RS21920 (D9C14_21920)	comER	4164066..4164887 (+)	822	WP_004398597.1	late competence protein ComER	-
D9C14_RS21925 (D9C14_21925)	yqeM	4164953..4165696 (-)	744	WP_003229973.1	class I SAM-dependent methyltransferase	-
D9C14_RS21930 (D9C14_21930)	rsfS	4165693..4166049 (-)	357	WP_003229971.1	ribosome silencing factor	-
D9C14_RS21935 (D9C14_21935)	yqeK	4166067..4166627 (-)	561	WP_004399059.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
D9C14_RS21940 (D9C14_21940)	nadD	4166617..4167186 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
D9C14_RS21945 (D9C14_21945)	yhbY	4167198..4167488 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
D9C14_RS21950 (D9C14_21950)	aroE	4167482..4168324 (-)	843	WP_003229967.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21769.45 Da
• Isoelectric Point: 4.7220

>NTDB_id=319528 D9C14_RS21915 WP_004398514.1 4163365..4163982(-) (comEA) [Bacillus subtilis subsp. subtilis strain SSJ-1]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=319528 D9C14_RS21915 WP_004398514.1 4163365..4163982(-) (comEA) [Bacillus subtilis subsp. subtilis strain SSJ-1]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAGGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (142-203)

• SLBB domain (SLBB) (63-118)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	100	100	1
comEA	Staphylococcus aureus MW2	37.273	100	0.4
comEA	Staphylococcus aureus N315	36.364	100	0.39