Detailed information NTG0319426

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: D9C12_RS15135
• Genome accession: NZ_CP032857
• Coordinates: 2809961..2810578 (-)
• Length: 205 a.a.
• NCBI ID: WP_046160608.1
• Uniprot ID: -
• Organism: Bacillus subtilis subsp. subtilis strain 2RL2-3
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2804961..2815578

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
D9C12_RS15105 (D9C12_15105)	rpsT	2805263..2805529 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
D9C12_RS15110 (D9C12_15110)	holA	2805544..2806587 (-)	1044	WP_014480310.1	DNA polymerase III subunit delta	-
D9C12_RS15115 (D9C12_15115)	-	2806627..2806776 (-)	150	WP_003229985.1	hypothetical protein	-
D9C12_RS15120 (D9C12_15120)	yqzM	2806817..2806951 (+)	135	WP_003229983.1	YqzM family protein	-
D9C12_RS15125 (D9C12_15125)	comEC	2806991..2809321 (-)	2331	WP_121509424.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
D9C12_RS15130 (D9C12_15130)	comEB	2809325..2809894 (-)	570	WP_003229978.1	ComE operon protein 2	-
D9C12_RS15135 (D9C12_15135)	comEA	2809961..2810578 (-)	618	WP_046160608.1	competence protein ComEA	Machinery gene
D9C12_RS15140 (D9C12_15140)	comER	2810662..2811483 (+)	822	WP_032726195.1	late competence protein ComER	-
D9C12_RS15145 (D9C12_15145)	yqeM	2811549..2812292 (-)	744	WP_069837641.1	class I SAM-dependent methyltransferase	-
D9C12_RS15150 (D9C12_15150)	rsfS	2812289..2812645 (-)	357	WP_014480315.1	ribosome silencing factor	-
D9C12_RS15155 (D9C12_15155)	yqeK	2812663..2813223 (-)	561	WP_014480316.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
D9C12_RS15160 (D9C12_15160)	nadD	2813213..2813782 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
D9C12_RS15165 (D9C12_15165)	yhbY	2813794..2814084 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
D9C12_RS15170 (D9C12_15170)	aroE	2814078..2814920 (-)	843	WP_014480317.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21738.48 Da
• Isoelectric Point: 4.7284

>NTDB_id=319426 D9C12_RS15135 WP_046160608.1 2809961..2810578(-) (comEA) [Bacillus subtilis subsp. subtilis strain 2RL2-3]
MNWLNQHKKAIVLAASAAVFTAIMIFLATGKNKEPVKQAVPTEAENTVVKQEANNDESSETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEEITKVSGIGEKLFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=319426 D9C12_RS15135 WP_046160608.1 2809961..2810578(-) (comEA) [Bacillus subtilis subsp. subtilis strain 2RL2-3]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTGTTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGGCAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAGCGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGAGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTTTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGAAATCACTAAGGT
TTCAGGAATAGGTGAAAAGTTATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (63-118)

• Helix-hairpin-helix motif (HHH_3) (142-203)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	97.561	100	0.976
comEA	Staphylococcus aureus MW2	35.909	100	0.385
comEA	Staphylococcus aureus N315	35	100	0.376