Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   D9C11_RS02530 Genome accession   NZ_CP032855
Coordinates   430905..431522 (-) Length   205 a.a.
NCBI ID   WP_014480312.1    Uniprot ID   -
Organism   Bacillus subtilis subsp. subtilis strain PJ-7     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 425905..436522
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D9C11_RS02500 (D9C11_02500) rpsT 426211..426477 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  D9C11_RS02505 (D9C11_02505) holA 426492..427535 (-) 1044 WP_041333763.1 DNA polymerase III subunit delta -
  D9C11_RS02510 (D9C11_02510) - 427575..427724 (-) 150 WP_003229985.1 hypothetical protein -
  D9C11_RS02515 (D9C11_02515) yqzM 427765..427899 (+) 135 WP_121590975.1 YqzM family protein -
  D9C11_RS02520 (D9C11_02520) comEC 427939..430265 (-) 2327 Protein_499 DNA internalization-related competence protein ComEC/Rec2 -
  D9C11_RS02525 (D9C11_02525) comEB 430269..430838 (-) 570 WP_003229978.1 ComE operon protein 2 -
  D9C11_RS02530 (D9C11_02530) comEA 430905..431522 (-) 618 WP_014480312.1 competence protein ComEA Machinery gene
  D9C11_RS02535 (D9C11_02535) comER 431606..432427 (+) 822 WP_014480313.1 late competence protein ComER -
  D9C11_RS02540 (D9C11_02540) yqeM 432493..433236 (-) 744 WP_003229973.1 class I SAM-dependent methyltransferase -
  D9C11_RS02545 (D9C11_02545) rsfS 433233..433589 (-) 357 WP_041333759.1 ribosome silencing factor -
  D9C11_RS02550 (D9C11_02550) yqeK 433607..434167 (-) 561 WP_014480316.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  D9C11_RS02555 (D9C11_02555) nadD 434157..434726 (-) 570 WP_041333756.1 nicotinate-nucleotide adenylyltransferase -
  D9C11_RS02560 (D9C11_02560) yhbY 434738..435028 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  D9C11_RS02565 (D9C11_02565) aroE 435022..435864 (-) 843 WP_029317883.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21781.51 Da        Isoelectric Point: 4.7220

>NTDB_id=319308 D9C11_RS02530 WP_014480312.1 430905..431522(-) (comEA) [Bacillus subtilis subsp. subtilis strain PJ-7]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEEIAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=319308 D9C11_RS02530 WP_014480312.1 430905..431522(-) (comEA) [Bacillus subtilis subsp. subtilis strain PJ-7]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAATAGCAGTGCAGCAAGGTGGCGGAGGGTCAG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

99.512

100

0.995
  comEA Staphylococcus aureus MW2

37.273

100

0.4
  comEA Staphylococcus aureus N315

36.364

100

0.39

Multiple sequence alignment