Detailed information NTG0319252

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: D9C10_RS11270
• Genome accession: NZ_CP032853
• Coordinates: 2039949..2040566 (-)
• Length: 205 a.a.
• NCBI ID: WP_014480312.1
• Uniprot ID: -
• Organism: Bacillus subtilis subsp. subtilis strain MH-1
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2034949..2045566

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
D9C10_RS11240 (D9C10_11240)	rpsT	2035251..2035517 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
D9C10_RS11245 (D9C10_11245)	holA	2035532..2036575 (-)	1044	WP_014480310.1	DNA polymerase III subunit delta	-
D9C10_RS11250 (D9C10_11250)	-	2036615..2036764 (-)	150	WP_121548944.1	hypothetical protein	-
D9C10_RS11255 (D9C10_11255)	yqzM	2036805..2036939 (+)	135	WP_003229983.1	YqzM family protein	-
D9C10_RS11260 (D9C10_11260)	comEC	2036979..2039309 (-)	2331	WP_033881047.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
D9C10_RS11265 (D9C10_11265)	comEB	2039313..2039882 (-)	570	WP_003229978.1	ComE operon protein 2	-
D9C10_RS11270 (D9C10_11270)	comEA	2039949..2040566 (-)	618	WP_014480312.1	competence protein ComEA	Machinery gene
D9C10_RS11275 (D9C10_11275)	comER	2040650..2041471 (+)	822	WP_014480313.1	late competence protein ComER	-
D9C10_RS11280 (D9C10_11280)	yqeM	2041537..2042280 (-)	744	WP_003229973.1	class I SAM-dependent methyltransferase	-
D9C10_RS11285 (D9C10_11285)	rsfS	2042277..2042633 (-)	357	WP_041333759.1	ribosome silencing factor	-
D9C10_RS11290 (D9C10_11290)	yqeK	2042651..2043211 (-)	561	WP_014480316.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
D9C10_RS11295 (D9C10_11295)	nadD	2043201..2043770 (-)	570	WP_041333756.1	nicotinate-nucleotide adenylyltransferase	-
D9C10_RS11300 (D9C10_11300)	yhbY	2043782..2044072 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
D9C10_RS11305 (D9C10_11305)	aroE	2044066..2044908 (-)	843	WP_029317883.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21781.51 Da
• Isoelectric Point: 4.7220

>NTDB_id=319252 D9C10_RS11270 WP_014480312.1 2039949..2040566(-) (comEA) [Bacillus subtilis subsp. subtilis strain MH-1]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEEIAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=319252 D9C10_RS11270 WP_014480312.1 2039949..2040566(-) (comEA) [Bacillus subtilis subsp. subtilis strain MH-1]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAATAGCAGTGCAGCAAGGTGGCGGAGGGTCAG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (142-203)

• SLBB domain (SLBB) (63-118)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	99.512	100	0.995
comEA	Staphylococcus aureus MW2	37.273	100	0.4
comEA	Staphylococcus aureus N315	36.364	100	0.39