Detailed information NTG0319199

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: D9C09_RS17320
• Genome accession: NZ_CP032852
• Coordinates: 3238258..3238875 (-)
• Length: 205 a.a.
• NCBI ID: WP_046160608.1
• Uniprot ID: -
• Organism: Bacillus subtilis subsp. subtilis strain GFR-12
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3233258..3243875

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
D9C09_RS17290 (D9C09_17290)	rpsT	3233560..3233826 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
D9C09_RS17295 (D9C09_17295)	holA	3233841..3234884 (-)	1044	WP_014480310.1	DNA polymerase III subunit delta	-
D9C09_RS17300 (D9C09_17300)	-	3234924..3235073 (-)	150	WP_003229985.1	hypothetical protein	-
D9C09_RS17305 (D9C09_17305)	yqzM	3235114..3235248 (+)	135	WP_003229983.1	YqzM family protein	-
D9C09_RS17310 (D9C09_17310)	comEC	3235288..3237618 (-)	2331	WP_069837640.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
D9C09_RS17315 (D9C09_17315)	comEB	3237622..3238191 (-)	570	WP_003229978.1	ComE operon protein 2	-
D9C09_RS17320 (D9C09_17320)	comEA	3238258..3238875 (-)	618	WP_046160608.1	competence protein ComEA	Machinery gene
D9C09_RS17325 (D9C09_17325)	comER	3238959..3239780 (+)	822	WP_032726195.1	late competence protein ComER	-
D9C09_RS17330 (D9C09_17330)	yqeM	3239846..3240589 (-)	744	WP_069837641.1	class I SAM-dependent methyltransferase	-
D9C09_RS17335 (D9C09_17335)	rsfS	3240586..3240942 (-)	357	WP_014480315.1	ribosome silencing factor	-
D9C09_RS17340 (D9C09_17340)	yqeK	3240960..3241520 (-)	561	WP_014480316.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
D9C09_RS17345 (D9C09_17345)	nadD	3241510..3242079 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
D9C09_RS17350 (D9C09_17350)	yhbY	3242091..3242381 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
D9C09_RS17355 (D9C09_17355)	aroE	3242375..3243217 (-)	843	WP_014480317.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21738.48 Da
• Isoelectric Point: 4.7284

>NTDB_id=319199 D9C09_RS17320 WP_046160608.1 3238258..3238875(-) (comEA) [Bacillus subtilis subsp. subtilis strain GFR-12]
MNWLNQHKKAIVLAASAAVFTAIMIFLATGKNKEPVKQAVPTEAENTVVKQEANNDESSETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEEITKVSGIGEKLFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=319199 D9C09_RS17320 WP_046160608.1 3238258..3238875(-) (comEA) [Bacillus subtilis subsp. subtilis strain GFR-12]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTGTTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGGCAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAGCGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGAGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTTTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGAAATCACTAAGGT
TTCAGGAATAGGTGAAAAGTTATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (63-118)

• Helix-hairpin-helix motif (HHH_3) (142-203)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	97.561	100	0.976
comEA	Staphylococcus aureus MW2	35.909	100	0.385
comEA	Staphylococcus aureus N315	35	100	0.376