Detailed information NTG0318402

Bioinformatically predicted

Overview

• Name: ssb
• Type: Machinery gene
• Locus tag: D8N35_RS03855
• Genome accession: NZ_CP032739
• Coordinates: 779233..779757 (-)
• Length: 174 a.a.
• NCBI ID: WP_005226365.1
• Uniprot ID: A0A505IXH5
• Organism: Enterococcus casseliflavus strain EC-369
• Function: ssDNA binding (predicted from homology) DNA processing

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Prophage	772781..787559	779233..779757	within	0

Gene organization within MGE regions

Location: 772781..787559

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
D8N35_RS03825 (D8N35_03825)	-	772781..773656 (-)	876	WP_005226378.1	DegV family protein	-
D8N35_RS03830 (D8N35_03830)	-	773797..775089 (-)	1293	WP_121260452.1	adenylosuccinate synthase	-
D8N35_RS03835 (D8N35_03835)	dnaB	775337..776707 (-)	1371	WP_005226374.1	replicative DNA helicase	-
D8N35_RS03840 (D8N35_03840)	rplI	776816..777268 (-)	453	WP_005226371.1	50S ribosomal protein L9	-
D8N35_RS03845 (D8N35_03845)	-	777284..778732 (-)	1449	WP_015508681.1	DHH family phosphoesterase	-
D8N35_RS03850 (D8N35_03850)	rpsR	778973..779209 (-)	237	WP_005226367.1	30S ribosomal protein S18	-
D8N35_RS03855 (D8N35_03855)	ssb	779233..779757 (-)	525	WP_005226365.1	single-stranded DNA-binding protein	Machinery gene
D8N35_RS03860 (D8N35_03860)	rpsF	779801..780100 (-)	300	WP_005226363.1	30S ribosomal protein S6	-
D8N35_RS03865 (D8N35_03865)	gyrA	780364..782889 (-)	2526	WP_015508679.1	DNA gyrase subunit A	-
D8N35_RS03870 (D8N35_03870)	gyrB	782913..784862 (-)	1950	WP_005226359.1	DNA topoisomerase (ATP-hydrolyzing) subunit B	-
D8N35_RS03875 (D8N35_03875)	recF	784868..785980 (-)	1113	WP_010748471.1	DNA replication/repair protein RecF	-
D8N35_RS03880 (D8N35_03880)	yaaA	785967..786206 (-)	240	WP_015508677.1	S4 domain-containing protein YaaA	-
D8N35_RS03885 (D8N35_03885)	dnaN	786429..787559 (-)	1131	WP_005226354.1	DNA polymerase III subunit beta	-

Sequence

Protein

• Length: 174 a.a.
• Molecular weight: 19149.65 Da
• Isoelectric Point: 4.7093

>NTDB_id=318402 D8N35_RS03855 WP_005226365.1 779233..779757(-) (ssb) [Enterococcus casseliflavus strain EC-369]
MINNVVLVGRLTKDPDLRYTSNGTGVATFTLAVNRNFTNQSGEREADFINCVIWRKPAETLANYARKGTLLGVTGRIQTR
SYDNQQGQRVYVTEVVADNFQLLESRNASEQRRTTDSGSGSNNTYNSNSSSGFGNNNSNQTQSSQPSGMPNFDRDNSDPF
GNSSIDISDDDLPF

Nucleotide

• Length: 525 bp

>NTDB_id=318402 D8N35_RS03855 WP_005226365.1 779233..779757(-) (ssb) [Enterococcus casseliflavus strain EC-369]
TTGATTAATAATGTTGTACTAGTAGGAAGACTAACCAAAGATCCTGATTTACGTTACACTTCTAATGGCACTGGTGTCGC
AACCTTTACCTTGGCGGTAAACCGCAATTTTACCAACCAAAGTGGAGAGCGCGAAGCTGATTTCATCAACTGTGTTATTT
GGAGAAAACCAGCGGAAACATTAGCAAACTATGCACGCAAAGGAACTTTATTAGGGGTTACTGGACGTATTCAAACCCGT
TCATACGACAACCAACAAGGCCAACGTGTTTATGTAACAGAAGTCGTTGCTGACAACTTCCAATTATTAGAGTCGCGGAA
TGCTTCCGAGCAAAGACGCACAACTGATAGCGGATCTGGCAGTAACAATACGTATAACTCAAACAGTTCTTCTGGATTTG
GTAACAATAACTCAAATCAAACTCAATCCTCTCAACCATCAGGAATGCCTAATTTTGATCGAGACAACTCCGATCCATTC
GGAAACTCATCAATCGACATTTCAGACGATGATTTACCATTCTAA

Domains

Predicted by InterproScan.

• Single-strand binding protein family (SSB) (2-103)

3D structure

• AlphaFold DB: A0A505IXH5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ssb	Latilactobacillus sakei subsp. sakei 23K	60.795	100	0.615
ssbA	Bacillus subtilis subsp. subtilis str. 168	55.866	100	0.575
ssbB	Bacillus subtilis subsp. subtilis str. 168	63.208	60.92	0.385