Detailed information NTG0317322

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: E7Z57_RS05360
• Genome accession: NZ_CP039339
• Coordinates: 1083888..1084769 (-)
• Length: 293 a.a.
• NCBI ID: WP_138928648.1
• Uniprot ID: -
• Organism: Ralstonia pseudosolanacearum strain UW386
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1078888..1089769

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
E7Z57_RS05330 (E7Z57_05330)	argJ	1079598..1080827 (+)	1230	WP_138928646.1	bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ	-
E7Z57_RS05335 (E7Z57_05335)	-	1080843..1081727 (+)	885	WP_138928647.1	ATP-binding protein	-
E7Z57_RS05340 (E7Z57_05340)	-	1081705..1082166 (+)	462	WP_064048488.1	NUDIX domain-containing protein	-
E7Z57_RS05345 (E7Z57_05345)	yacG	1082159..1082374 (-)	216	WP_011002736.1	DNA gyrase inhibitor YacG	-
E7Z57_RS05350 (E7Z57_05350)	zapD	1082385..1083143 (-)	759	WP_064048489.1	cell division protein ZapD	-
E7Z57_RS05355 (E7Z57_05355)	coaE	1083261..1083875 (-)	615	WP_064048490.1	dephospho-CoA kinase	-
E7Z57_RS05360 (E7Z57_05360)	pilD	1083888..1084769 (-)	882	WP_138928648.1	prepilin peptidase	Machinery gene
E7Z57_RS05365 (E7Z57_05365)	pilC	1084772..1086037 (-)	1266	WP_064048492.1	type II secretion system F family protein	Machinery gene
E7Z57_RS05370 (E7Z57_05370)	pilF	1086082..1087809 (-)	1728	WP_064048493.1	type IV-A pilus assembly ATPase PilB	Machinery gene
E7Z57_RS05375 (E7Z57_05375)	-	1088057..1089334 (-)	1278	WP_138928649.1	HlyC/CorC family transporter	-

Sequence

Protein

• Length: 293 a.a.
• Molecular weight: 31605.60 Da
• Isoelectric Point: 8.3415

>NTDB_id=317322 E7Z57_RS05360 WP_138928648.1 1083888..1084769(-) (pilD) [Ralstonia pseudosolanacearum strain UW386]
MPGMFVIPTLAQLPAWFVIGAGTLLGLLVGSFLNVVIHRLPRMIEREEANYIAELRNEPLPHPEPYNLVVPRSACPSCGH
QIKAVENIPVLSWLALRGRCSACKTPISWRYPAVELVTGALTGACLWHFGPTWVAVASAALLWFLIAGSMIDADTQLLPD
AITQPLLWLGLLVNLFGMFARLPDAVIGATAGYLFLWTIYWAYKLLRGREGMGYGDFKLMGALGAWFGWQALPLLILLSS
VVGLVFGVVRIARGATSESPFSFGPFIAGAGVISLLAGPQLVALMGLAPLLAR

Nucleotide

• Length: 882 bp

>NTDB_id=317322 E7Z57_RS05360 WP_138928648.1 1083888..1084769(-) (pilD) [Ralstonia pseudosolanacearum strain UW386]
ATGCCCGGAATGTTCGTCATTCCCACGCTGGCGCAACTGCCGGCGTGGTTCGTGATCGGCGCGGGGACGCTGCTGGGCTT
GCTGGTCGGCAGCTTCCTCAACGTGGTGATTCACCGCCTGCCGCGCATGATCGAGCGCGAAGAGGCCAACTACATCGCCG
AGCTGCGCAACGAGCCGCTGCCGCATCCCGAGCCATACAACCTGGTCGTGCCGCGCTCGGCCTGCCCGTCGTGCGGCCAC
CAGATCAAGGCGGTCGAGAACATCCCGGTGCTGAGCTGGCTGGCGTTGCGCGGCCGCTGCAGCGCCTGCAAGACCCCCAT
CTCCTGGCGCTATCCCGCCGTGGAGCTGGTGACCGGCGCGCTGACCGGCGCCTGCCTGTGGCACTTCGGGCCGACCTGGG
TGGCGGTGGCCTCGGCCGCGCTGCTGTGGTTCCTGATCGCCGGCTCGATGATCGACGCCGACACGCAGCTCCTGCCCGAT
GCCATCACCCAGCCGCTGCTGTGGCTCGGCCTGCTGGTGAACCTGTTCGGCATGTTTGCTCGGCTGCCGGACGCGGTAAT
CGGCGCGACGGCCGGGTATCTGTTCCTGTGGACCATCTACTGGGCCTATAAGCTGCTGCGCGGCCGGGAAGGCATGGGCT
ACGGCGACTTCAAGCTGATGGGCGCACTGGGCGCCTGGTTCGGCTGGCAGGCGCTGCCGCTGCTGATACTGCTGTCATCG
GTGGTCGGCCTGGTGTTCGGGGTGGTGCGCATCGCGCGCGGCGCCACGAGCGAATCGCCGTTCTCGTTCGGCCCGTTCAT
CGCGGGTGCCGGCGTGATCTCACTGCTGGCCGGCCCGCAATTGGTGGCGCTGATGGGGCTTGCGCCACTGCTGGCGCGGT
AA

Domains

Predicted by InterProScan.

• Type IV leader peptidase family (Peptidase_A24) (140-248)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (24-129)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	52.075	90.444	0.471
pilD	Vibrio campbellii strain DS40M4	49.819	94.539	0.471
pilD	Neisseria gonorrhoeae MS11	49.254	91.468	0.451
pilD	Acinetobacter nosocomialis M2	45.113	90.785	0.41
pilD	Acinetobacter baumannii D1279779	45.113	90.785	0.41