Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   D7D53_RS09245 Genome accession   NZ_CP032621
Coordinates   1828329..1829627 (+) Length   432 a.a.
NCBI ID   WP_120770771.1    Uniprot ID   A0A387B4D2
Organism   Streptococcus gwangjuensis strain KCOM 1679 (=ChDC B345)     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1823329..1834627
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D7D53_RS09220 (D7D53_09220) rpsB 1824132..1824911 (+) 780 WP_000268469.1 30S ribosomal protein S2 -
  D7D53_RS09225 (D7D53_09225) tsf 1824989..1826029 (+) 1041 WP_000808071.1 translation elongation factor Ts -
  D7D53_RS09230 (D7D53_09230) cysK 1826155..1827081 (-) 927 WP_049521100.1 cysteine synthase A -
  D7D53_RS09235 (D7D53_09235) - 1827178..1827621 (-) 444 WP_000863906.1 PH domain-containing protein -
  D7D53_RS09240 (D7D53_09240) - 1827637..1828272 (-) 636 WP_049491126.1 YigZ family protein -
  D7D53_RS09245 (D7D53_09245) comFA/cflA 1828329..1829627 (+) 1299 WP_120770771.1 DEAD/DEAH box helicase Machinery gene
  D7D53_RS09250 (D7D53_09250) comFC/cflB 1829624..1830286 (+) 663 WP_070842124.1 ComF family protein Machinery gene
  D7D53_RS09255 (D7D53_09255) hpf 1830366..1830914 (+) 549 WP_004255322.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  D7D53_RS09260 (D7D53_09260) - 1831050..1833023 (+) 1974 WP_120770772.1 DHH family phosphoesterase -
  D7D53_RS09265 (D7D53_09265) rplI 1833020..1833472 (+) 453 WP_120770773.1 50S ribosomal protein L9 -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 49683.58 Da        Isoelectric Point: 8.4404

>NTDB_id=317231 D7D53_RS09245 WP_120770771.1 1828329..1829627(+) (comFA/cflA) [Streptococcus gwangjuensis strain KCOM 1679 (=ChDC B345)]
MKVNPNYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCDSAILDEWYLPIGAYYCRECLIMKRIRSDQALYYFPQ
EDFPKQDVLKWRGQLTPFQEKVSKGLIQAVDKQEPTLVHAVTGAGKTEMIYQVVAKVIDEGGAVCLASPRIDVCLELYKR
LQDDFACEIALLHGESEPYFRTPLVVATTHQLLKFYHAFDLLIVDEVDAFPYVDNPTLYHAVKNSVKKNGLRIFLTATST
DELDRKVRIGELKRLSLPRRFHGNPLIIPKPVWLSDFNRYLDKNRLSPKLKFYIEKQRKTGYPLLIFASEIKKGEQLKEV
LQKEFSHEKIGFVSSVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGGRVGRSMDR
PTGDLLFFHDGLNASIKKAIKEIQQMNKEASL

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=317231 D7D53_RS09245 WP_120770771.1 1828329..1829627(+) (comFA/cflA) [Streptococcus gwangjuensis strain KCOM 1679 (=ChDC B345)]
ATGAAAGTAAATCCAAATTATCTCGGTCGGTTGTTTACGGAGAATGAATTAACAGAAGAGGAACGTCAGTTGGCGGAGAA
ACTTCCAGCAATGAGAAAGGAGAAAGGGAAACTTTTCTGTCAACGTTGTGATAGTGCTATTTTAGATGAATGGTATTTGC
CCATCGGTGCTTACTATTGTCGGGAGTGCTTAATTATGAAGCGAATTAGGAGTGATCAAGCTTTATACTATTTCCCACAG
GAGGATTTTCCGAAGCAAGATGTTCTTAAATGGCGTGGTCAATTAACTCCTTTTCAAGAGAAGGTGTCAAAGGGATTGAT
TCAAGCGGTAGATAAGCAGGAACCAACCTTGGTTCACGCTGTAACAGGAGCTGGAAAGACAGAGATGATTTACCAAGTTG
TGGCCAAAGTGATTGATGAAGGTGGTGCAGTTTGTTTGGCCAGCCCTAGAATCGATGTGTGTTTGGAGCTGTATAAGCGA
CTGCAAGATGATTTTGCTTGCGAGATAGCTTTACTACACGGAGAATCAGAACCATATTTTCGAACACCACTAGTTGTTGC
AACGACTCATCAGCTGTTAAAATTTTATCATGCTTTTGATTTGCTAATAGTGGATGAAGTAGATGCTTTTCCTTATGTTG
ACAACCCTACGCTTTACCATGCTGTCAAGAATAGTGTAAAGAAGAATGGCTTGAGAATCTTTTTAACAGCAACTTCTACC
GATGAGTTAGATAGGAAGGTTCGCATCGGAGAATTAAAACGATTGAGTTTGCCTAGACGGTTTCATGGAAATCCTTTGAT
TATTCCTAAACCTGTCTGGTTATCCGATTTTAATCGCTATTTGGATAAGAATCGTTTATCACCAAAGTTAAAGTTTTATA
TTGAGAAACAGAGAAAGACAGGTTATCCGTTACTCATTTTTGCTTCAGAAATTAAGAAAGGGGAGCAATTGAAAGAAGTC
TTACAGAAGGAATTTTCGCATGAGAAAATTGGTTTTGTGTCTTCAGTTACGGAAGATCGATTAGAGCAGGTGCAAGCTTT
TCGAGATGGAGAACTGACAATACTTATCAGTACGACAATCTTGGAGCGTGGAGTTACCTTCCCTTGTGTGGATGTTTTTG
TAGTAGAAGCCAACCATCGTCTGTTTACCAAATCTAGTTTGATTCAGATTGGTGGACGAGTTGGACGAAGCATGGATAGA
CCGACAGGAGATTTGCTTTTCTTCCATGACGGGTTAAATGCTTCAATCAAAAAGGCAATTAAGGAAATTCAACAGATGAA
TAAGGAGGCGAGTCTATGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(94-238)

Helicase conserved C-terminal domain (Helicase_C)

(294-396)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A387B4D2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus mitis SK321

94.444

100

0.944
  comFA/cflA Streptococcus mitis NCTC 12261

92.361

100

0.924
  comFA/cflA Streptococcus pneumoniae Rx1

92.13

100

0.921
  comFA/cflA Streptococcus pneumoniae D39

92.13

100

0.921
  comFA/cflA Streptococcus pneumoniae R6

92.13

100

0.921
  comFA/cflA Streptococcus pneumoniae TIGR4

91.898

100

0.919
  comFA Lactococcus lactis subsp. cremoris KW2

50.118

98.38

0.493
  comFA Latilactobacillus sakei subsp. sakei 23K

38.747

99.769

0.387
  comFA Bacillus subtilis subsp. subtilis str. 168

38.806

93.056

0.361

Multiple sequence alignment