Detailed information NTG0316528

Bioinformatically predicted

Overview

• Name: comGA
• Type: Machinery gene
• Locus tag: D5H27_RS09600
• Genome accession: NZ_CP032506
• Coordinates: 2009072..2010142 (-)
• Length: 356 a.a.
• NCBI ID: WP_003153083.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain JT3-1
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2004072..2015142

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
D5H27_RS09550 (D5H27_09430)	-	2004131..2004715 (-)	585	WP_032874025.1	signal peptidase I	-
D5H27_RS09555 (D5H27_09435)	tapA	2004687..2005358 (-)	672	WP_032874023.1	amyloid fiber anchoring/assembly protein TapA	-
D5H27_RS09560 (D5H27_09440)	-	2005617..2005946 (+)	330	WP_032874021.1	DUF3889 domain-containing protein	-
D5H27_RS09565 (D5H27_09445)	-	2005987..2006166 (-)	180	WP_022552966.1	YqzE family protein	-
D5H27_RS09570 (D5H27_09450)	comGG	2006223..2006600 (-)	378	WP_032874019.1	competence type IV pilus minor pilin ComGG	Machinery gene
D5H27_RS09575 (D5H27_09455)	comGF	2006601..2007101 (-)	501	WP_223204419.1	competence type IV pilus minor pilin ComGF	-
D5H27_RS09580 (D5H27_09460)	comGE	2007010..2007324 (-)	315	WP_032874016.1	competence type IV pilus minor pilin ComGE	Machinery gene
D5H27_RS09585 (D5H27_09465)	comGD	2007308..2007745 (-)	438	WP_007612572.1	competence type IV pilus minor pilin ComGD	Machinery gene
D5H27_RS09590 (D5H27_09470)	comGC	2007735..2008001 (-)	267	WP_042635730.1	competence type IV pilus major pilin ComGC	Machinery gene
D5H27_RS09595 (D5H27_09475)	comGB	2008048..2009085 (-)	1038	WP_032874014.1	competence type IV pilus assembly protein ComGB	Machinery gene
D5H27_RS09600 (D5H27_09480)	comGA	2009072..2010142 (-)	1071	WP_003153083.1	competence type IV pilus ATPase ComGA	Machinery gene
D5H27_RS09605 (D5H27_09485)	-	2010339..2011289 (-)	951	WP_032874012.1	magnesium transporter CorA family protein	-
D5H27_RS09610 (D5H27_09490)	-	2011435..2012736 (+)	1302	WP_032874010.1	hemolysin family protein	-
D5H27_RS09615 (D5H27_09495)	-	2012774..2013604 (-)	831	WP_032874008.1	STAS domain-containing protein	-
D5H27_RS09625 (D5H27_09505)	-	2013810..2014190 (-)	381	WP_032874006.1	Spx/MgsR family RNA polymerase-binding regulatory protein	-
D5H27_RS09630 (D5H27_09510)	-	2014427..2014672 (+)	246	WP_003153078.1	DUF2626 domain-containing protein	-

Sequence

Protein

• Length: 356 a.a.
• Molecular weight: 40529.85 Da
• Isoelectric Point: 9.4729

>NTDB_id=316528 D5H27_RS09600 WP_003153083.1 2009072..2010142(-) (comGA) [Bacillus velezensis strain JT3-1]
MKNIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide

• Length: 1071 bp

>NTDB_id=316528 D5H27_RS09600 WP_003153083.1 2009072..2010142(-) (comGA) [Bacillus velezensis strain JT3-1]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCATATACGGCAAGGGCTTCAGATATACACATTGTACC
GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTTATTTCTCATTTCAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGGAAGCCGCAAAACGGGTCATTGTCT
TTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAATTGTCCTTATTTCGGGGGGCGGGCGCCGCATTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCCACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAAAGGCATTTCAACCGGCGTATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTTGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC
TATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (10-275)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGA	Bacillus subtilis subsp. subtilis str. 168	82.022	100	0.82
pilB	Glaesserella parasuis strain SC1401	38.793	97.753	0.379
pilF	Thermus thermophilus HB27	37.892	98.596	0.374