Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ECD_RS15970 Genome accession   NC_012971
Coordinates   3213217..3214650 (+) Length   477 a.a.
NCBI ID   WP_000809051.1    Uniprot ID   A0A0H2VDV8
Organism   Escherichia coli BL21(DE3)     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3208217..3219650
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ECD_RS15940 (ECD_03061) yrbG 3208775..3209752 (+) 978 WP_000922900.1 calcium/sodium antiporter -
  ECD_RS15945 (ECD_03062) kdsD 3209766..3210752 (+) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  ECD_RS15950 (ECD_03063) kdsC 3210773..3211339 (+) 567 WP_000030016.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  ECD_RS15955 (ECD_03064) lptC 3211336..3211911 (+) 576 WP_000030530.1 LPS export ABC transporter periplasmic protein LptC -
  ECD_RS15960 (ECD_03065) lptA 3211880..3212437 (+) 558 WP_000669774.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  ECD_RS15965 (ECD_03066) lptB 3212444..3213169 (+) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  ECD_RS15970 (ECD_03067) rpoN/rpoN1 3213217..3214650 (+) 1434 WP_000809051.1 RNA polymerase factor sigma-54 Machinery gene
  ECD_RS15975 (ECD_03068) hpf 3214673..3214960 (+) 288 WP_001176599.1 ribosome hibernation promoting factor -
  ECD_RS15980 (ECD_03069) ptsN 3215078..3215569 (+) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ECD_RS15985 (ECD_03070) rapZ 3215615..3216469 (+) 855 WP_000243741.1 RNase adapter RapZ -
  ECD_RS15990 (ECD_03071) npr 3216466..3216738 (+) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  ECD_RS15995 (ECD_03072) yrbL 3216952..3217584 (+) 633 WP_000620405.1 PhoP regulatory network protein YrbL -
  ECD_RS16000 (ECD_03073) mtgA 3217581..3218309 (-) 729 WP_000047091.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  ECD_RS16005 (ECD_03074) elbB 3218306..3218959 (-) 654 WP_001300411.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53993.79 Da        Isoelectric Point: 4.3639

>NTDB_id=31353 ECD_RS15970 WP_000809051.1 3213217..3214650(+) (rpoN/rpoN1) [Escherichia coli BL21(DE3)]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=31353 ECD_RS15970 WP_000809051.1 3213217..3214650(+) (rpoN/rpoN1) [Escherichia coli BL21(DE3)]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
TGAGCTGCCGGTCTATCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTCGAGCTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATCGACATCGACGAGGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTAGCGGCAAAAGATCTGCGTGACTGCCTGCTAATCCAACTCTCCCAATTCGATAAAACCACGCCGTGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAACTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VDV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465

Multiple sequence alignment