Detailed information NTG0313075

Bioinformatically predicted

Overview

• Name: comE
• Type: Machinery gene
• Locus tag: AhaeAN4_RS01550
• Genome accession: NZ_CP031979
• Coordinates: 334426..334923 (+)
• Length: 165 a.a.
• NCBI ID: WP_004641267.1
• Uniprot ID: N9FBJ9
• Organism: Acinetobacter haemolyticus strain AN4
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
IScluster/Tn	335640..342277	334426..334923	flank	717

Gene organization within MGE regions

Location: 334426..342277

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AhaeAN4_RS01550 (AhaeAN4_01575)	comE	334426..334923 (+)	498	WP_004641267.1	type IV pilin protein	Machinery gene
AhaeAN4_RS01555 (AhaeAN4_01580)	-	334917..335375 (+)	459	WP_004641266.1	type IV pilin protein	-
AhaeAN4_RS01565 (AhaeAN4_01595)	-	336839..337429 (+)	591	WP_160132021.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
AhaeAN4_RS01570 (AhaeAN4_01600)	-	337517..338649 (+)	1133	WP_227554019.1	IS3 family transposase	-
AhaeAN4_RS01575 (AhaeAN4_01605)	pilV	338717..339205 (+)	489	WP_004641261.1	type IV pilus modification protein PilV	Machinery gene
AhaeAN4_RS01580 (AhaeAN4_01610)	comB	339390..340337 (+)	948	WP_008941160.1	PilW family protein	Machinery gene
AhaeAN4_RS01585 (AhaeAN4_01615)	pilX	340340..341074 (+)	735	WP_004641259.1	PilX N-terminal domain-containing pilus assembly protein	Machinery gene

Sequence

Protein

• Length: 165 a.a.
• Molecular weight: 17952.36 Da
• Isoelectric Point: 8.4798

>NTDB_id=313075 AhaeAN4_RS01550 WP_004641267.1 334426..334923(+) (comE) [Acinetobacter haemolyticus strain AN4]
MQNNRLTSAQGFTLVELMIVVVIVAIFAAIAIPSYQEYVRRAIAAQAQQEMQRISTELERSKARNFNYLGFPSTVVLPVG
ATGAAIQYTITVSDGADVSRSLTDSAATGQSWVMRGIWKTAETTNVDNFSFLMTSSGVRCKNKTKANITATNVDNATCGT
GREEW

Nucleotide

• Length: 498 bp

>NTDB_id=313075 AhaeAN4_RS01550 WP_004641267.1 334426..334923(+) (comE) [Acinetobacter haemolyticus strain AN4]
ATGCAAAATAATCGGTTAACCTCTGCTCAAGGGTTTACTTTGGTTGAACTAATGATTGTTGTCGTTATTGTTGCAATTTT
TGCAGCAATAGCGATACCAAGTTATCAAGAATATGTTCGGCGTGCGATTGCTGCGCAAGCTCAGCAGGAAATGCAACGCA
TATCAACTGAACTGGAAAGAAGTAAAGCTAGAAACTTCAATTATTTGGGGTTTCCGAGTACGGTTGTTTTGCCCGTTGGT
GCAACAGGTGCTGCGATTCAATATACGATTACTGTTAGTGACGGAGCAGATGTGAGTAGATCACTAACAGATAGTGCAGC
AACAGGACAAAGCTGGGTCATGCGCGGTATTTGGAAAACAGCCGAAACAACAAATGTGGATAACTTTAGTTTTTTGATGA
CCAGTTCAGGAGTCCGCTGTAAAAATAAAACCAAGGCAAACATTACAGCAACGAATGTCGATAATGCGACATGTGGTACA
GGGCGAGAAGAATGGTAA

Domains

Predicted by InterproScan.

• Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS) (33-97)

• Prokaryotic N-terminal methylation motif (N_methyl) (9-31)

3D structure

• AlphaFold DB: N9FBJ9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comE	Acinetobacter baylyi ADP1	43.243	100	0.485
pilY2	Acinetobacter baumannii D1279779	45.455	100	0.455