Detailed information    

insolico Bioinformatically predicted

Overview

Name   crp   Type   Regulator
Locus tag   E4U70_RS02385 Genome accession   NZ_CP038363
Coordinates   512743..513375 (-) Length   210 a.a.
NCBI ID   WP_000242755.1    Uniprot ID   A0A1C4FZU4
Organism   Escherichia coli O157:H7 strain F7349     
Function   promote expression of competence genes (predicted from homology)   
Competence regulation

Genomic Context

Location: 507743..518375
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E4U70_RS02360 (E4U70_02430) yhfG 507875..508042 (+) 168 WP_000736862.1 YhfG family protein -
  E4U70_RS02365 (E4U70_02435) fic 508032..508634 (+) 603 WP_001280650.1 putative adenosine monophosphate-protein transferase Fic -
  E4U70_RS02370 (E4U70_02440) pabA 508666..509229 (+) 564 WP_000601867.1 aminodeoxychorismate synthase component 2 -
  E4U70_RS02375 (E4U70_02445) argD 509315..510535 (+) 1221 WP_000963819.1 bifunctional acetylornithine/succinyldiaminopimelate transaminase -
  E4U70_RS02380 (E4U70_02450) yhfK 510602..512692 (-) 2091 WP_001295162.1 YccS/YhfK family putative transporter -
  E4U70_RS02385 (E4U70_02455) crp 512743..513375 (-) 633 WP_000242755.1 cAMP-activated global transcriptional regulator CRP Regulator
  E4U70_RS02390 (E4U70_02460) yhfA 513677..514081 (+) 405 WP_001148908.1 OsmC family protein -
  E4U70_RS02395 (E4U70_02465) prkB 514136..515005 (-) 870 WP_001274677.1 phosphoribulokinase -
  E4U70_RS02400 (E4U70_02470) yheU 515059..515277 (-) 219 WP_000907085.1 YheU family protein -
  E4U70_RS02405 (E4U70_02475) yheT 515271..516293 (-) 1023 WP_000057377.1 hydrolase -
  E4U70_RS02410 (E4U70_02480) yheS 516293..518206 (-) 1914 WP_000634798.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 210 a.a.        Molecular weight: 23640.43 Da        Isoelectric Point: 8.2710

>NTDB_id=313048 E4U70_RS02385 WP_000242755.1 512743..513375(-) (crp) [Escherichia coli O157:H7 strain F7349]
MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIVKGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEG
QERSAWVRAKTACEVAEISYKKFRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR

Nucleotide


Download         Length: 633 bp        

>NTDB_id=313048 E4U70_RS02385 WP_000242755.1 512743..513375(-) (crp) [Escherichia coli O157:H7 strain F7349]
ATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAA
GAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCTGTGCTGATCAAAG
ACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGC
CAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGAT
TCAGGTAAACCCGGACATTCTGATGCGTCTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCA
ACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTCAATCTGGCAAAACAACCAGACGCTATGACTCAC
CCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATCGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCAT
TCTGAAGATGCTTGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAA

Domains

Predicted by InterProScan.

Cyclic nucleotide-binding domain (cNMP_binding)

(23-109)

Crp-like helix-turn-helix domain (HTH_Crp_2)

(142-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1C4FZU4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  crp Vibrio cholerae strain A1552

95.714

100

0.957
  crp Haemophilus influenzae Rd KW20

77.941

97.143

0.757
  crp Acinetobacter baumannii D1279779

47.343

98.571

0.467

Multiple sequence alignment