Detailed information    

insolico Bioinformatically predicted

Overview

Name   crp   Type   Regulator
Locus tag   E4U77_RS02480 Genome accession   NZ_CP038346
Coordinates   528740..529372 (-) Length   210 a.a.
NCBI ID   WP_000242755.1    Uniprot ID   A0A1C4FZU4
Organism   Escherichia coli O157:H7 strain G5295     
Function   promote expression of competence genes (predicted from homology)   
Competence regulation

Genomic Context

Location: 523740..534372
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E4U77_RS02455 (E4U77_02525) yhfG 523872..524039 (+) 168 WP_000736862.1 YhfG family protein -
  E4U77_RS02460 (E4U77_02530) fic 524029..524631 (+) 603 WP_001280650.1 putative adenosine monophosphate-protein transferase Fic -
  E4U77_RS02465 (E4U77_02535) pabA 524663..525226 (+) 564 WP_000601867.1 aminodeoxychorismate synthase component 2 -
  E4U77_RS02470 (E4U77_02540) argD 525312..526532 (+) 1221 WP_000963819.1 bifunctional acetylornithine/succinyldiaminopimelate transaminase -
  E4U77_RS02475 (E4U77_02545) yhfK 526599..528689 (-) 2091 WP_001295162.1 YccS/YhfK family putative transporter -
  E4U77_RS02480 (E4U77_02550) crp 528740..529372 (-) 633 WP_000242755.1 cAMP-activated global transcriptional regulator CRP Regulator
  E4U77_RS02485 (E4U77_02555) yhfA 529674..530078 (+) 405 WP_001148908.1 OsmC family protein -
  E4U77_RS02490 (E4U77_02560) prkB 530133..531002 (-) 870 WP_001274677.1 phosphoribulokinase -
  E4U77_RS02495 (E4U77_02565) yheU 531056..531274 (-) 219 WP_000907085.1 YheU family protein -
  E4U77_RS02500 (E4U77_02570) yheT 531268..532290 (-) 1023 WP_000057377.1 hydrolase -
  E4U77_RS02505 (E4U77_02575) yheS 532290..534203 (-) 1914 WP_000634798.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 210 a.a.        Molecular weight: 23640.43 Da        Isoelectric Point: 8.2710

>NTDB_id=312501 E4U77_RS02480 WP_000242755.1 528740..529372(-) (crp) [Escherichia coli O157:H7 strain G5295]
MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIVKGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEG
QERSAWVRAKTACEVAEISYKKFRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR

Nucleotide


Download         Length: 633 bp        

>NTDB_id=312501 E4U77_RS02480 WP_000242755.1 528740..529372(-) (crp) [Escherichia coli O157:H7 strain G5295]
ATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAA
GAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCTGTGCTGATCAAAG
ACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGC
CAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGAT
TCAGGTAAACCCGGACATTCTGATGCGTCTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCA
ACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTCAATCTGGCAAAACAACCAGACGCTATGACTCAC
CCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATCGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCAT
TCTGAAGATGCTTGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAA

Domains

Predicted by InterProScan.

Cyclic nucleotide-binding domain (cNMP_binding)

(23-109)

Crp-like helix-turn-helix domain (HTH_Crp_2)

(142-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1C4FZU4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  crp Vibrio cholerae strain A1552

95.714

100

0.957
  crp Haemophilus influenzae Rd KW20

77.941

97.143

0.757
  crp Acinetobacter baumannii D1279779

47.343

98.571

0.467

Multiple sequence alignment