Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   E3163_RS02490 Genome accession   NZ_CP038342
Coordinates   530325..530792 (-) Length   155 a.a.
NCBI ID   WP_000381746.1    Uniprot ID   Q8X892
Organism   Escherichia coli O157:H7 strain H2495     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 525325..535792
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E3163_RS02465 (E3163_02535) rpsG 525466..525936 (+) 471 WP_001138043.1 30S ribosomal protein S7 -
  E3163_RS02470 (E3163_02540) fusA 526033..528147 (+) 2115 WP_000124700.1 elongation factor G -
  E3163_RS02475 (E3163_02545) tuf 528218..529402 (+) 1185 WP_000031783.1 elongation factor Tu -
  E3163_RS02480 (E3163_02550) bfd 529585..529779 (+) 195 WP_000289090.1 bacterioferritin-associated ferredoxin -
  E3163_RS02485 (E3163_02555) bfr 529852..530328 (+) 477 WP_000675516.1 bacterioferritin -
  E3163_RS02490 (E3163_02560) pilD 530325..530792 (-) 468 WP_000381746.1 A24 family peptidase Machinery gene
  E3163_RS02495 (E3163_02570) rpsJ 531171..531482 (+) 312 WP_001181004.1 30S ribosomal protein S10 -
  E3163_RS02500 (E3163_02575) rplC 531515..532144 (+) 630 WP_000579833.1 50S ribosomal protein L3 -
  E3163_RS02505 (E3163_02580) rplD 532155..532760 (+) 606 WP_000424395.1 50S ribosomal protein L4 -
  E3163_RS02510 (E3163_02585) rplW 532757..533059 (+) 303 WP_000617544.1 50S ribosomal protein L23 -
  E3163_RS02515 (E3163_02590) rplB 533077..533898 (+) 822 WP_000301864.1 50S ribosomal protein L2 -
  E3163_RS02520 (E3163_02595) rpsS 533915..534193 (+) 279 WP_001138117.1 30S ribosomal protein S19 -
  E3163_RS02525 (E3163_02600) rplV 534208..534540 (+) 333 WP_000447529.1 50S ribosomal protein L22 -
  E3163_RS02530 (E3163_02605) rpsC 534558..535259 (+) 702 WP_000529945.1 30S ribosomal protein S3 -
  E3163_RS02535 (E3163_02610) rplP 535272..535682 (+) 411 WP_000941212.1 50S ribosomal protein L16 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 17099.29 Da        Isoelectric Point: 8.1343

>NTDB_id=312344 E3163_RS02490 WP_000381746.1 530325..530792(-) (pilD) [Escherichia coli O157:H7 strain H2495]
MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFYQVCHPDGLADALWGAIIGYGTFAVIYWGYRILRHKE
GLGYGDVKFLAALGAWHSWAFLPRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR

Nucleotide


Download         Length: 468 bp        

>NTDB_id=312344 E3163_RS02490 WP_000381746.1 530325..530792(-) (pilD) [Escherichia coli O157:H7 strain H2495]
ATGGACACTACCCTTCCCTTTCTAATTTTGTACGCTTGTTTATCAGCCCTACTTTTTTTCTGGGATGCAAAGCATGGCTT
GTTACCTGACAGATTTACGTGCCCGTTACTCTGGTCAGGTCTTTTGTTCTATCAAGTTTGTCATCCTGATGGTTTAGCCG
ATGCGTTATGGGGGGCAATTATTGGTTACGGCACATTCGCTGTCATTTACTGGGGGTATCGCATACTGCGCCATAAAGAA
GGATTAGGCTATGGTGATGTGAAGTTTCTCGCCGCCCTTGGCGCCTGGCATTCATGGGCCTTCTTACCACGACTGGTTTT
CCTTGCTGCCTCATTCGCTTGCGGAGCCGTAGTTATTGGTCTGCTCATGAGAGGAAAAGAATCATTAAAAAACCCGCTGC
CTTTTGGGCCATTTCTGGCGGCTGCGGGTTTCGTTATAGGCTGGGATAGTTTGCTGATAGGCCGTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(11-117)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8X892

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

43.478

89.032

0.387

Multiple sequence alignment