Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   D1111_RS04365 Genome accession   NZ_CP031855
Coordinates   978430..978876 (-) Length   148 a.a.
NCBI ID   WP_215577633.1    Uniprot ID   -
Organism   Actinobacillus pleuropneumoniae strain 7311555     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 973430..983876
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D1111_RS04340 (D1111_04355) - 973959..974597 (-) 639 WP_005604550.1 prepilin peptidase -
  D1111_RS04345 (D1111_04360) - 974624..975394 (-) 771 Protein_846 type II secretion system F family protein -
  D1111_RS04350 (D1111_04365) - 975493..976533 (+) 1041 WP_005603118.1 IS481 family transposase -
  D1111_RS04355 (D1111_04370) - 976591..977034 (-) 444 Protein_848 type II secretion system F family protein -
  D1111_RS04360 (D1111_04375) pilB 977018..978412 (-) 1395 WP_005601209.1 GspE/PulE family protein Machinery gene
  D1111_RS04365 (D1111_04380) pilA 978430..978876 (-) 447 WP_215577633.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  D1111_RS04370 (D1111_04385) radA 979058..980437 (+) 1380 WP_005601211.1 DNA repair protein RadA Machinery gene
  D1111_RS04375 (D1111_04390) - 980481..980714 (-) 234 WP_005597399.1 YgjV family protein -
  D1111_RS04380 (D1111_04395) folA 980841..981329 (-) 489 WP_005597400.1 type 3 dihydrofolate reductase -
  D1111_RS04385 (D1111_04400) proB 981416..982507 (+) 1092 WP_005601213.1 glutamate 5-kinase -
  D1111_RS04390 (D1111_04405) - 982520..983296 (+) 777 WP_237610649.1 TSUP family transporter -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 15962.35 Da        Isoelectric Point: 7.6257

>NTDB_id=311918 D1111_RS04365 WP_215577633.1 978430..978876(-) (pilA) [Actinobacillus pleuropneumoniae strain 7311555]
MQKLSLIRPLTNAFTLIELMIVIAIIAILATVAIPSYNSYTQKAALSELLAASASYKTDVEICIYNTGDSKNCSGGQNGV
RKITELRQAKYLNAITVEGGTITVTGKGNLQEYGYTMTPIHNGSTISWETKCKGEDLSLFPANFCASN

Nucleotide


Download         Length: 447 bp        

>NTDB_id=311918 D1111_RS04365 WP_215577633.1 978430..978876(-) (pilA) [Actinobacillus pleuropneumoniae strain 7311555]
ATGCAAAAACTAAGTCTTATTCGACCGCTTACTAACGCGTTTACTTTAATTGAATTGATGATCGTGATTGCGATTATTGC
CATTTTAGCTACGGTTGCAATTCCGTCATATAACAGTTATACCCAAAAAGCGGCGCTTTCGGAGCTATTGGCGGCATCGG
CTTCTTATAAAACGGATGTCGAGATCTGCATATATAACACCGGAGATTCTAAAAACTGTAGCGGCGGTCAAAACGGTGTC
AGAAAAATAACGGAGCTTAGACAGGCTAAATATTTAAATGCCATTACGGTGGAAGGCGGAACGATTACAGTAACGGGGAA
AGGGAATCTACAGGAATACGGTTATACGATGACACCGATTCATAACGGTAGCACTATTTCTTGGGAAACGAAATGTAAAG
GGGAGGACTTAAGTTTATTTCCGGCAAATTTCTGCGCATCAAATTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(13-33)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

68.919

100

0.689
  pilA Haemophilus influenzae 86-028NP

55.102

99.324

0.547
  pilA Haemophilus influenzae Rd KW20

53.061

99.324

0.527

Multiple sequence alignment