Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   D0819_RS08785 Genome accession   NZ_CP031784
Coordinates   1672411..1673028 (-) Length   205 a.a.
NCBI ID   WP_077671454.1    Uniprot ID   -
Organism   Bacillus subtilis strain HMNig-2     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1667411..1678028
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D0819_RS08755 (D0819_08775) rpsT 1667713..1667979 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  D0819_RS08760 (D0819_08780) holA 1667994..1669037 (-) 1044 WP_029317888.1 DNA polymerase III subunit delta -
  D0819_RS08765 - 1669077..1669226 (-) 150 WP_003229985.1 hypothetical protein -
  D0819_RS08770 (D0819_08785) yqzM 1669267..1669401 (+) 135 WP_003229983.1 YqzM family protein -
  D0819_RS08775 (D0819_08790) comEC 1669441..1671771 (-) 2331 WP_153256924.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  D0819_RS08780 (D0819_08795) comEB 1671775..1672344 (-) 570 WP_003229978.1 ComE operon protein 2 -
  D0819_RS08785 (D0819_08800) comEA 1672411..1673028 (-) 618 WP_077671454.1 competence protein ComEA Machinery gene
  D0819_RS08790 (D0819_08805) comER 1673112..1673933 (+) 822 WP_021480064.1 late competence protein ComER -
  D0819_RS08795 (D0819_08810) yqeM 1673999..1674742 (-) 744 WP_072174289.1 class I SAM-dependent methyltransferase -
  D0819_RS08800 (D0819_08815) rsfS 1674739..1675095 (-) 357 WP_014477391.1 ribosome silencing factor -
  D0819_RS08805 (D0819_08820) yqeK 1675113..1675673 (-) 561 WP_014480316.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  D0819_RS08810 (D0819_08825) nadD 1675663..1676232 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  D0819_RS08815 (D0819_08830) yhbY 1676244..1676534 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  D0819_RS08820 (D0819_08835) aroE 1676528..1677370 (-) 843 WP_014480317.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21756.45 Da        Isoelectric Point: 4.7284

>NTDB_id=311383 D0819_RS08785 WP_077671454.1 1672411..1673028(-) (comEA) [Bacillus subtilis strain HMNig-2]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESSETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEEITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=311383 D0819_RS08785 WP_077671454.1 1672411..1673028(-) (comEA) [Bacillus subtilis strain HMNig-2]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAGCGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCAAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGAAATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

99.024

100

0.99
  comEA Staphylococcus aureus MW2

36.818

100

0.395
  comEA Staphylococcus aureus N315

35.455

100

0.38

Multiple sequence alignment