Detailed information NTG0310405

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: DXY21_RS11780
• Genome accession: NZ_CP031694
• Coordinates: 2308118..2308732 (+)
• Length: 204 a.a.
• NCBI ID: WP_015387983.1
• Uniprot ID: A0A6A8LHQ3
• Organism: Bacillus velezensis strain SRCM101368
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2303118..2313732

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DXY21_RS11745 (DXY21_02344)	aroE	2303805..2304647 (+)	843	WP_160249250.1	shikimate dehydrogenase	-
DXY21_RS11750 (DXY21_02345)	yhbY	2304641..2304931 (+)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
DXY21_RS11755 (DXY21_02346)	-	2304942..2305511 (+)	570	WP_160249251.1	nicotinate-nucleotide adenylyltransferase	-
DXY21_RS11760 (DXY21_02347)	yqeK	2305501..2306082 (+)	582	WP_003152863.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
DXY21_RS11765 (DXY21_02348)	rsfS	2306079..2306435 (+)	357	WP_003152864.1	ribosome silencing factor	-
DXY21_RS11770 (DXY21_02349)	-	2306432..2307169 (+)	738	WP_014305450.1	class I SAM-dependent DNA methyltransferase	-
DXY21_RS11775 (DXY21_02350)	comER	2307238..2308059 (-)	822	WP_160249252.1	late competence protein ComER	-
DXY21_RS11780 (DXY21_02351)	comEA	2308118..2308732 (+)	615	WP_015387983.1	helix-hairpin-helix domain-containing protein	Machinery gene
DXY21_RS11785 (DXY21_02352)	-	2308799..2309368 (+)	570	WP_003152868.1	ComE operon protein 2	-
DXY21_RS11790 (DXY21_02353)	comEC	2309369..2311720 (+)	2352	WP_069473513.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
DXY21_RS11795	-	2311739..2311873 (-)	135	WP_003152870.1	YqzM family protein	-
DXY21_RS11800	-	2311914..2312068 (+)	155	Protein_2269	hypothetical protein	-
DXY21_RS11805 (DXY21_02354)	holA	2312108..2313149 (+)	1042	Protein_2270	DNA polymerase III subunit delta	-
DXY21_RS11810 (DXY21_02355)	rpsT	2313166..2313432 (-)	267	WP_003152876.1	30S ribosomal protein S20	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22255.41 Da
• Isoelectric Point: 5.5211

>NTDB_id=310405 DXY21_RS11780 WP_015387983.1 2308118..2308732(+) (comEA) [Bacillus velezensis strain SRCM101368]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=310405 DXY21_RS11780 WP_015387983.1 2308118..2308732(+) (comEA) [Bacillus velezensis strain SRCM101368]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: A0A6A8LHQ3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363