Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   DXY22_RS15955 Genome accession   NZ_CP031693
Coordinates   3129381..3129998 (-) Length   205 a.a.
NCBI ID   WP_004398514.1    Uniprot ID   A0AAE2SLR2
Organism   Bacillus subtilis strain SRCM101393     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3124381..3134998
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DXY22_RS15925 (DXY22_03134) rpsT 3124683..3124949 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  DXY22_RS15930 (DXY22_03135) holA 3124964..3126007 (-) 1044 WP_015251674.1 DNA polymerase III subunit delta -
  DXY22_RS15935 - 3126047..3126196 (-) 150 WP_003229985.1 hypothetical protein -
  DXY22_RS15940 (DXY22_03136) yqzM 3126237..3126371 (+) 135 WP_003229983.1 YqzM family protein -
  DXY22_RS15945 (DXY22_03137) comEC 3126411..3128741 (-) 2331 WP_015251673.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  DXY22_RS15950 (DXY22_03138) comEB 3128745..3129314 (-) 570 WP_003229978.1 ComE operon protein 2 -
  DXY22_RS15955 (DXY22_03139) comEA 3129381..3129998 (-) 618 WP_004398514.1 competence protein ComEA Machinery gene
  DXY22_RS15960 (DXY22_03140) comER 3130082..3130903 (+) 822 WP_004398597.1 late competence protein ComER -
  DXY22_RS15965 (DXY22_03141) yqeM 3130969..3131712 (-) 744 WP_003229973.1 class I SAM-dependent methyltransferase -
  DXY22_RS15970 (DXY22_03142) rsfS 3131709..3132065 (-) 357 WP_003229971.1 ribosome silencing factor -
  DXY22_RS15975 (DXY22_03143) yqeK 3132083..3132643 (-) 561 WP_004399059.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  DXY22_RS15980 (DXY22_03144) nadD 3132633..3133202 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  DXY22_RS15985 (DXY22_03145) yhbY 3133214..3133504 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  DXY22_RS15990 (DXY22_03146) aroE 3133498..3134340 (-) 843 WP_003229967.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21769.45 Da        Isoelectric Point: 4.7220

>NTDB_id=310348 DXY22_RS15955 WP_004398514.1 3129381..3129998(-) (comEA) [Bacillus subtilis strain SRCM101393]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=310348 DXY22_RS15955 WP_004398514.1 3129381..3129998(-) (comEA) [Bacillus subtilis strain SRCM101393]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAGGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

100

100

1
  comEA Staphylococcus aureus MW2

37.273

100

0.4
  comEA Staphylococcus aureus N315

36.364

100

0.39

Multiple sequence alignment