Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CH578_RS03930 Genome accession   NZ_CP031690
Coordinates   755628..757022 (+) Length   464 a.a.
NCBI ID   WP_011271972.1    Uniprot ID   -
Organism   Haemophilus influenzae strain P617-9224     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 750628..762022
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CH578_RS03905 (CH578_03905) rsmE 750647..751384 (-) 738 WP_011271976.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  CH578_RS03910 (CH578_03910) lnt 751434..752963 (-) 1530 WP_079989936.1 apolipoprotein N-acyltransferase -
  CH578_RS03915 (CH578_03915) corC 752986..753885 (-) 900 WP_005656283.1 CNNM family magnesium/cobalt transport protein CorC -
  CH578_RS03920 (CH578_03920) ampD 754507..755067 (-) 561 WP_070828280.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  CH578_RS03925 (CH578_03925) pilA 755182..755631 (+) 450 WP_011271973.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  CH578_RS03930 (CH578_03930) pilB 755628..757022 (+) 1395 WP_011271972.1 GspE/PulE family protein Machinery gene
  CH578_RS03935 (CH578_03935) pilC 757019..758239 (+) 1221 WP_011271971.1 type II secretion system F family protein Machinery gene
  CH578_RS03940 (CH578_03940) pilD 758236..758928 (+) 693 WP_038440789.1 prepilin peptidase Machinery gene
  CH578_RS03945 (CH578_03945) rho 758982..760244 (-) 1263 WP_005629550.1 transcription termination factor Rho -
  CH578_RS03950 (CH578_03950) metJ 760492..760809 (+) 318 WP_005631186.1 met regulon transcriptional regulator MetJ -
  CH578_RS03955 (CH578_03955) cueR 760823..761209 (-) 387 WP_005663006.1 Cu(I)-responsive transcriptional regulator -
  CH578_RS03960 (CH578_03960) - 761286..761492 (+) 207 WP_005631184.1 heavy-metal-associated domain-containing protein -
  CH578_RS03965 (CH578_03965) - 761567..761773 (+) 207 WP_005686717.1 heavy-metal-associated domain-containing protein -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 52962.30 Da        Isoelectric Point: 5.6248

>NTDB_id=310203 CH578_RS03930 WP_011271972.1 755628..757022(+) (pilB) [Haemophilus influenzae strain P617-9224]
MTSYALLHTQRVTAQNGEIFTISPDLWERNQQQQSLLLRYFALPLKEENNRLWLGVDSLSNLSACETIAFITGKPVEPIL
LESSQLKELLQQLTPHQMQVEEQVKFYQHQETHFEQEDDEPVIRLLNQIFESALQKNASDIHLETLADQFQVRFRIDGVL
QPQPLISKIFANRIISRLKLLAKLDISENRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKIVLRAQQNKPVELSFAELG
MTENQQQAFQRSLSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIELDGIIQSQINPQIGLDFSRLLR
AFLRQDPDIIMLGEIRDEESARIALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQQHEIENSLLLVIAQRLVRKICPKC
GGNLINSCDCHQGYRGRIGVYQFLHWQQNGYQTDFENLRESGLEKVSQGITDEKEIERVLGKNS

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=310203 CH578_RS03930 WP_011271972.1 755628..757022(+) (pilB) [Haemophilus influenzae strain P617-9224]
ATGACGAGCTATGCTTTACTTCATACTCAGCGTGTAACCGCTCAAAATGGCGAGATCTTTACGATCTCGCCAGATTTATG
GGAACGCAATCAGCAGCAACAATCCTTGCTCTTGCGGTATTTTGCTTTGCCACTTAAAGAAGAAAATAATCGTCTTTGGC
TAGGGGTTGATTCTCTCTCCAATCTTTCAGCTTGTGAAACCATTGCGTTTATAACAGGAAAACCTGTCGAACCAATTTTG
TTAGAAAGCAGCCAACTCAAAGAACTGTTACAACAACTTACTCCGCACCAAATGCAAGTGGAAGAGCAAGTTAAATTCTA
TCAACATCAAGAAACCCATTTTGAACAAGAAGATGATGAACCTGTTATCCGCTTACTTAATCAGATTTTTGAATCTGCCT
TACAAAAAAATGCCTCTGATATTCATTTAGAAACCTTGGCTGATCAGTTTCAAGTGCGGTTTAGAATTGATGGTGTTTTA
CAACCACAACCCTTAATAAGCAAAATATTCGCCAATCGTATTATTTCACGCTTAAAATTACTGGCTAAATTAGATATTAG
TGAAAATCGACTTCCACAAGATGGACGATTTCAATTTAAAACCACTTTTTCCGATATTCTTGATTTTCGCCTTTCAACCT
TACCAACCCATTGGGGCGAAAAAATCGTGTTGCGAGCGCAACAAAATAAACCTGTAGAACTTAGCTTTGCTGAACTGGGT
ATGACCGAAAATCAGCAACAAGCATTTCAACGCTCACTTAGCCAGCCACAAGGATTAATTTTAGTAACCGGCCCCACAGG
AAGTGGGAAAAGTATCTCGCTTTACACCGCACTTCAGTGGCTAAATACGCCTGATAAACATATTATGACCGCTGAAGATC
CCATTGAAATTGAACTTGATGGTATTATTCAAAGCCAAATTAATCCGCAGATTGGATTAGATTTTAGCCGTCTATTGCGT
GCTTTTTTACGTCAAGATCCCGACATCATTATGCTAGGTGAAATTCGAGATGAAGAAAGTGCAAGGATTGCACTACGTGC
CGCTCAAACGGGACATTTGGTGCTTTCAACTTTACATACCAATGATGCAATATCTGCCATTTCTCGCTTACAACAACTCG
GTATTCAACAACATGAAATTGAAAACAGTTTACTACTCGTCATTGCACAGCGTCTTGTACGAAAAATCTGTCCAAAGTGC
GGTGGAAATTTAATAAATTCTTGTGATTGCCATCAAGGTTATCGAGGGCGAATCGGCGTGTATCAATTTCTACATTGGCA
ACAGAATGGCTATCAAACGGATTTTGAGAATTTACGAGAGAGTGGTTTGGAAAAAGTTAGCCAAGGCATAACAGATGAGA
AAGAAATTGAACGTGTGTTAGGTAAAAACTCATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(24-98)

Type II/IV secretion system protein (T2SSE)

(128-395)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

100

100

1
  pilB Haemophilus influenzae Rd KW20

96.544

99.784

0.963
  pilB Glaesserella parasuis strain SC1401

57.456

98.276

0.565
  pilB Vibrio cholerae strain A1552

39.658

100

0.45
  pilB Vibrio campbellii strain DS40M4

40.082

100

0.422
  pilB Vibrio parahaemolyticus RIMD 2210633

39.959

100

0.416
  pilB Legionella pneumophila strain ERS1305867

39.468

100

0.416
  pilB Acinetobacter baylyi ADP1

38.105

100

0.407
  pilB Acinetobacter baumannii D1279779

38.669

100

0.401
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.393

100

0.377
  pilF Neisseria gonorrhoeae MS11

36.555

100

0.375
  pilF Thermus thermophilus HB27

36.134

100

0.371

Multiple sequence alignment