Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   CH613_RS03695 Genome accession   NZ_CP031684
Coordinates   714772..715464 (+) Length   230 a.a.
NCBI ID   WP_005686720.1    Uniprot ID   -
Organism   Haemophilus influenzae strain P652-8881     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 709772..720464
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CH613_RS03675 (CH613_03675) ampD 711045..711605 (-) 561 WP_005648974.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  CH613_RS03680 (CH613_03680) pilA 711720..712169 (+) 450 WP_005686726.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  CH613_RS03685 (CH613_03685) pilB 712166..713563 (+) 1398 WP_011961745.1 GspE/PulE family protein Machinery gene
  CH613_RS03690 (CH613_03690) - 713560..714775 (+) 1216 Protein_704 type II secretion system F family protein -
  CH613_RS03695 (CH613_03695) pilD 714772..715464 (+) 693 WP_005686720.1 A24 family peptidase Machinery gene
  CH613_RS03700 (CH613_03700) rho 715518..716780 (-) 1263 WP_005648966.1 transcription termination factor Rho -
  CH613_RS03705 (CH613_03705) metJ 717028..717345 (+) 318 WP_005631186.1 met regulon transcriptional regulator MetJ -
  CH613_RS03710 (CH613_03710) cueR 717359..717745 (-) 387 WP_005654127.1 Cu(I)-responsive transcriptional regulator -
  CH613_RS03715 (CH613_03715) - 717822..718028 (+) 207 WP_005631184.1 heavy-metal-associated domain-containing protein -
  CH613_RS03720 (CH613_03720) - 718114..718320 (+) 207 WP_005631184.1 heavy-metal-associated domain-containing protein -
  CH613_RS03725 (CH613_03725) - 718406..718612 (+) 207 WP_005631184.1 heavy-metal-associated domain-containing protein -
  CH613_RS03730 (CH613_03730) - 718698..718904 (+) 207 WP_005631184.1 heavy-metal-associated domain-containing protein -

Sequence

Protein


Download         Length: 230 a.a.        Molecular weight: 26677.71 Da        Isoelectric Point: 8.8394

>NTDB_id=309994 CH613_RS03695 WP_005686720.1 714772..715464(+) (pilD) [Haemophilus influenzae strain P652-8881]
MIYFTMFLLGGIFGIALWFYLSGFITRLQQEIYATYIELFPQNFLPFQPSVSSIQQKKCGHILRYFLNVGVGFIFLQIAF
KDSIFAIWLGLTLLILWAISYLDWYYQLISTTPCLWLLTIGLFGADNNFSLLTLSESIKSAASFFIVFYAIYWIAKFYYG
KEAFGRGDYWLAMALGSFIHLETLPHFLLLASVLGICFSLIHKKKKEFIPFAPFMNLSAVIIYFVKYYGY

Nucleotide


Download         Length: 693 bp        

>NTDB_id=309994 CH613_RS03695 WP_005686720.1 714772..715464(+) (pilD) [Haemophilus influenzae strain P652-8881]
ATGATTTACTTCACAATGTTTTTATTAGGCGGCATCTTCGGGATCGCATTGTGGTTCTACCTATCTGGTTTTATTACCCG
TTTACAGCAAGAGATTTATGCGACTTATATTGAATTATTCCCACAAAACTTTCTGCCATTTCAGCCTAGCGTTTCCTCTA
TTCAACAAAAAAAGTGCGGTCATATTTTGAGGTATTTTTTAAATGTTGGGGTAGGATTTATATTTTTACAAATTGCTTTC
AAGGACTCTATTTTCGCTATATGGCTAGGGCTCACACTTCTTATCCTTTGGGCAATTAGTTATCTTGATTGGTACTATCA
ACTTATTTCTACTACACCCTGTTTATGGCTACTTACAATCGGTTTATTTGGGGCAGACAATAATTTTTCATTGCTTACGT
TATCTGAAAGTATAAAAAGTGCGGCAAGTTTTTTTATTGTTTTCTACGCAATCTATTGGATTGCAAAATTCTATTACGGA
AAAGAAGCCTTTGGACGGGGAGATTATTGGCTAGCAATGGCATTAGGAAGTTTTATTCATTTAGAAACTTTGCCGCACTT
TTTATTATTAGCCTCAGTTCTTGGAATATGTTTTTCGCTTATTCATAAAAAGAAAAAAGAATTTATACCTTTTGCCCCTT
TTATGAACTTATCAGCTGTCATCATTTATTTCGTCAAATATTACGGATATTAA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(92-199)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Haemophilus influenzae 86-028NP

90.435

100

0.904
  pilD Haemophilus influenzae Rd KW20

90

100

0.9

Multiple sequence alignment