Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   CH635_RS04000 Genome accession   NZ_CP031679
Coordinates   767091..767783 (+) Length   230 a.a.
NCBI ID   WP_038440789.1    Uniprot ID   A0AAJ8WR53
Organism   Haemophilus influenzae strain P676-2514     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 762091..772783
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CH635_RS03980 (CH635_03975) ampD 763362..763922 (-) 561 WP_011271974.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  CH635_RS03985 (CH635_03980) pilA 764037..764486 (+) 450 WP_011271973.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  CH635_RS03990 (CH635_03985) pilB 764483..765877 (+) 1395 WP_011271972.1 GspE/PulE family protein Machinery gene
  CH635_RS03995 (CH635_03990) pilC 765874..767094 (+) 1221 WP_011271971.1 type II secretion system F family protein Machinery gene
  CH635_RS04000 (CH635_03995) pilD 767091..767783 (+) 693 WP_038440789.1 prepilin peptidase Machinery gene
  CH635_RS04005 (CH635_04000) rho 767837..769099 (-) 1263 WP_005629550.1 transcription termination factor Rho -
  CH635_RS04010 (CH635_04005) metJ 769347..769664 (+) 318 WP_005631186.1 met regulon transcriptional regulator MetJ -
  CH635_RS04015 (CH635_04010) cueR 769678..770064 (-) 387 WP_005663006.1 Cu(I)-responsive transcriptional regulator -
  CH635_RS04020 (CH635_04015) - 770141..770347 (+) 207 WP_005686717.1 heavy-metal-associated domain-containing protein -
  CH635_RS04025 (CH635_04020) - 770421..770627 (+) 207 WP_005631184.1 heavy-metal-associated domain-containing protein -
  CH635_RS04030 (CH635_04025) - 770602..772770 (+) 2169 WP_038440794.1 heavy metal translocating P-type ATPase -

Sequence

Protein


Download         Length: 230 a.a.        Molecular weight: 26786.77 Da        Isoelectric Point: 8.6895

>NTDB_id=309823 CH635_RS04000 WP_038440789.1 767091..767783(+) (pilD) [Haemophilus influenzae strain P676-2514]
MIYFTMFLLGGILGIALWFYLSGFITHLQQEIYATYVELFPQNSSPFQPHFASIQQKKCGHILRYFFSIGVGFIFLQIAF
KDSIFTVWIGLTLIILWTISYLDWHYQLISTTPCLWLLTLGLFGTDNNFSLLTLSESIKSAAIFFIVFYAIYWIAKCYYR
KEAFGRGDYWLAMALGSFIHLETLPHFLLLASVLGICFSLIHRKKKEFLPFAPFMNLSAVIIYFVKYYGY

Nucleotide


Download         Length: 693 bp        

>NTDB_id=309823 CH635_RS04000 WP_038440789.1 767091..767783(+) (pilD) [Haemophilus influenzae strain P676-2514]
ATGATTTACTTCACAATGTTTTTATTAGGCGGCATCTTAGGGATCGCATTGTGGTTCTACCTATCTGGTTTTATTACGCA
TTTGCAGCAAGAGATTTATGCGACTTACGTTGAATTATTTCCACAAAACAGTTCTCCATTTCAACCGCACTTTGCCTCTA
TTCAACAAAAAAAGTGCGGTCATATTTTGAGGTATTTTTTTAGTATTGGGGTTGGATTTATATTTTTACAAATTGCCTTC
AAAGATTCTATTTTTACTGTATGGATCGGACTCACACTTATTATTCTTTGGACAATCAGTTATCTTGATTGGCACTATCA
ACTTATTTCTACGACACCCTGTTTATGGTTACTTACTCTAGGATTATTTGGCACAGACAATAACTTTTCATTGCTAACGT
TATCTGAAAGCATAAAAAGTGCGGCTATTTTTTTTATTGTTTTCTACGCAATCTATTGGATTGCAAAATGTTATTACAGA
AAAGAAGCCTTTGGACGGGGAGATTATTGGCTAGCAATGGCATTAGGAAGTTTTATTCATTTAGAAACCTTACCGCACTT
TTTATTATTAGCCTCAGTTCTTGGAATATGTTTTTCGCTTATTCATAGAAAGAAAAAAGAATTTTTACCTTTTGCCCCTT
TTATGAACTTATCAGCTGTCATCATTTATTTCGTCAAATATTACGGATATTAA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(92-199)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Haemophilus influenzae 86-028NP

97.391

100

0.974
  pilD Haemophilus influenzae Rd KW20

95.652

100

0.957

Multiple sequence alignment