Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   DD545_RS07925 Genome accession   NZ_CP031664
Coordinates   1571485..1571982 (-) Length   165 a.a.
NCBI ID   WP_001789864.1    Uniprot ID   -
Organism   Staphylococcus aureus strain 28     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1566485..1576982
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DD545_RS07900 (DD545_01437) gcvPB 1566647..1568119 (-) 1473 WP_000202189.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  DD545_RS07905 (DD545_01438) gcvPA 1568112..1569458 (-) 1347 WP_000019687.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  DD545_RS07910 (DD545_01439) gcvT 1569478..1570569 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  DD545_RS07915 (DD545_01440) - 1570728..1571252 (-) 525 WP_001015117.1 shikimate kinase -
  DD545_RS07920 - 1571242..1571388 (-) 147 WP_001789879.1 hypothetical protein -
  DD545_RS07925 (DD545_01441) comGF 1571485..1571982 (-) 498 WP_001789864.1 competence type IV pilus minor pilin ComGF Machinery gene
  DD545_RS07930 (DD545_01442) comGE 1571900..1572199 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  DD545_RS07935 (DD545_01443) comGD 1572186..1572632 (-) 447 WP_001790850.1 competence type IV pilus minor pilin ComGD Machinery gene
  DD545_RS07940 (DD545_01444) comGC 1572610..1572921 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  DD545_RS07945 (DD545_01445) comGB 1572935..1574004 (-) 1070 Protein_1498 competence type IV pilus assembly protein ComGB -
  DD545_RS07950 (DD545_01446) comGA 1573976..1574950 (-) 975 WP_117207247.1 competence type IV pilus ATPase ComGA Machinery gene
  DD545_RS07955 (DD545_01447) - 1575002..1575625 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  DD545_RS07960 (DD545_01448) - 1575622..1575951 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  DD545_RS07965 (DD545_01449) - 1575951..1576937 (-) 987 WP_000161314.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19164.88 Da        Isoelectric Point: 10.2192

>NTDB_id=309538 DD545_RS07925 WP_001789864.1 1571485..1571982(-) (comGF) [Staphylococcus aureus strain 28]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRDLTTVDFEFFSRDI
LDDFKGVDRNDIEIRQHRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=309538 DD545_RS07925 WP_001789864.1 1571485..1571982(-) (comGF) [Staphylococcus aureus strain 28]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGTAAAACTTTTCTAATTGAAAGTAGGGATTTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGATGATTTTAAAGGAGTAGATAGAAACGATATTGAAATTAGGCAACACCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATTAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(67-155)

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus MW2

100

100

1
  comGF Staphylococcus aureus N315

96.364

100

0.964

Multiple sequence alignment