Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   DD545_RS07950 Genome accession   NZ_CP031664
Coordinates   1573976..1574950 (-) Length   324 a.a.
NCBI ID   WP_117207247.1    Uniprot ID   -
Organism   Staphylococcus aureus strain 28     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1568976..1579950
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DD545_RS07910 (DD545_01439) gcvT 1569478..1570569 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  DD545_RS07915 (DD545_01440) - 1570728..1571252 (-) 525 WP_001015117.1 shikimate kinase -
  DD545_RS07920 - 1571242..1571388 (-) 147 WP_001789879.1 hypothetical protein -
  DD545_RS07925 (DD545_01441) comGF 1571485..1571982 (-) 498 WP_001789864.1 competence type IV pilus minor pilin ComGF Machinery gene
  DD545_RS07930 (DD545_01442) comGE 1571900..1572199 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  DD545_RS07935 (DD545_01443) comGD 1572186..1572632 (-) 447 WP_001790850.1 competence type IV pilus minor pilin ComGD Machinery gene
  DD545_RS07940 (DD545_01444) comGC 1572610..1572921 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  DD545_RS07945 (DD545_01445) comGB 1572935..1574004 (-) 1070 Protein_1498 competence type IV pilus assembly protein ComGB -
  DD545_RS07950 (DD545_01446) comGA 1573976..1574950 (-) 975 WP_117207247.1 competence type IV pilus ATPase ComGA Machinery gene
  DD545_RS07955 (DD545_01447) - 1575002..1575625 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  DD545_RS07960 (DD545_01448) - 1575622..1575951 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  DD545_RS07965 (DD545_01449) - 1575951..1576937 (-) 987 WP_000161314.1 ROK family glucokinase -
  DD545_RS07970 - 1576934..1577137 (-) 204 WP_000087561.1 YqgQ family protein -
  DD545_RS07975 (DD545_01450) - 1577118..1578581 (-) 1464 WP_001017867.1 rhomboid family intramembrane serine protease -
  DD545_RS07980 (DD545_01451) - 1578593..1579132 (-) 540 WP_000164545.1 5-formyltetrahydrofolate cyclo-ligase -
  DD545_RS07985 (DD545_01452) rpmG 1579341..1579490 (-) 150 WP_001265709.1 50S ribosomal protein L33 -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36899.03 Da        Isoelectric Point: 9.0399

>NTDB_id=309542 DD545_RS07950 WP_117207247.1 1573976..1574950(-) (comGA) [Staphylococcus aureus strain 28]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYLF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQPQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQFRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTM
HKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=309542 DD545_RS07950 WP_117207247.1 1573976..1574950(-) (comGA) [Staphylococcus aureus strain 28]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCTTTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTTATCAGAATTGTACCTCA
ATTTTTTCAACCACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGACCAACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATCGTCCAAATTAATGTGAATGATAAAGCTGGCATAAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTATCTCAGCAACAATTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
CATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

98.765

100

0.988
  comGA Staphylococcus aureus N315

98.765

100

0.988

Multiple sequence alignment