Detailed information NTG0307427

Bioinformatically predicted

Overview

• Name: amiA
• Type: Regulator
• Locus tag: DV947_RS06690
• Genome accession: NZ_CP031545
• Coordinates: 1262729..1264696 (-)
• Length: 655 a.a.
• NCBI ID: WP_116920313.1
• Uniprot ID: -
• Organism: Streptococcus thermophilus strain ST109
• Function: internalize XIP (predicted from homology) Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Prophage	1265052..1280502	1262729..1264696	flank	356
IScluster/Tn	1265052..1274928	1262729..1264696	flank	356

Gene organization within MGE regions

Location: 1262729..1280502

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DV947_RS06690 (DV947_06695)	amiA	1262729..1264696 (-)	1968	WP_116920313.1	peptide ABC transporter substrate-binding protein	Regulator
DV947_RS06695 (DV947_06700)	-	1265052..1266229 (+)	1178	Protein_1237	ISL3 family transposase	-
DV947_RS06700 (DV947_06705)	-	1266354..1267603 (+)	1250	Protein_1238	ISL3 family transposase	-
DV947_RS06705 (DV947_06710)	amiA3	1267693..1269666 (-)	1974	WP_116920314.1	peptide ABC transporter substrate-binding protein	Regulator
DV947_RS06710 (DV947_06715)	-	1269971..1271118 (+)	1148	WP_095559415.1	IS3 family transposase	-
DV947_RS06715 (DV947_06720)	-	1271156..1271340 (-)	185	Protein_1241	IS3 family transposase	-
DV947_RS10305	-	1271334..1271735 (-)	402	Protein_1242	ABC transporter ATP-binding protein	-
DV947_RS06730 (DV947_06735)	-	1271730..1272090 (-)	361	Protein_1243	TatD family hydrolase	-
DV947_RS06735 (DV947_06740)	-	1272436..1273641 (+)	1206	WP_011681420.1	OFA family MFS transporter	-
DV947_RS06740 (DV947_06745)	-	1273687..1275000 (-)	1314	Protein_1245	IS3 family transposase	-
DV947_RS06745 (DV947_06750)	pta	1275125..1276108 (-)	984	WP_024010014.1	phosphate acetyltransferase	-
DV947_RS06750 (DV947_06755)	-	1276118..1277017 (-)	900	WP_116920315.1	RluA family pseudouridine synthase	-
DV947_RS06755 (DV947_06760)	-	1277014..1277850 (-)	837	WP_014608552.1	NAD kinase	-
DV947_RS06760 (DV947_06765)	-	1277822..1278496 (-)	675	WP_011681427.1	GTP pyrophosphokinase family protein	-
DV947_RS06765 (DV947_06770)	-	1278592..1279167 (+)	576	WP_002951444.1	CYTH domain-containing protein	-
DV947_RS06770 (DV947_06775)	-	1279531..1280502 (+)	972	WP_116920316.1	ribose-phosphate diphosphokinase	-

Sequence

Protein

• Length: 655 a.a.
• Molecular weight: 71701.84 Da
• Isoelectric Point: 5.1059

>NTDB_id=307427 DV947_RS06690 WP_116920313.1 1262729..1264696(-) (amiA) [Streptococcus thermophilus strain ST109]
MKLKKILGITGVALLSVGMLAACSSKSSTSGTTYSNIYGSDPETLDYITSIMGGTKAVLTNGVDGLMEADKYGNLVPSVA
EDWSVSKDGLTYTYKIRKGIKWYTSEGEEYANVTAKDFVTGLKHAADAKSGALYLVQDSIAGLSDYLSGANKDFSNVGVK
AIDDYTLQYTLKKQEPYWNSKTTYGVLFPVNEDFLKNKGKDFGKSTDPTSILYNGPFLLKSLTAKSSIELTKNENYWDKK
NVHFDSIKFSYSDGSDLESIERSFSDGALSIARVFPTSSNYASVEKKYKDNIFYTEPGASTSVIGVNIDRQSYKFSAKKT
DAEKTSTKKALLNKDFRQSINFAIDRTAYQSQVNGKDGAALALRNLFVPYDFVSAGDKTFGDLVTEKMSSYGDEWSGVNF
ADGQEGLYNAEKAKTEFAKAKEALQGEGVQFPVHLDLPVDQSSKLSVAQAQSLKQTIEKSLGSENVVIDINQMSSDDMNN
VTSNAANAAAEDWDISNGVGWGPDYQDPSTYLDILKTTSSENTKIFNGYDDPNNAAAAQVGLKNYDALLDSAASETTDIN
ARYDRYAQAQAWLEDSSLIIPLTVSNGAAPVISRLTPFTGASIQVGDKGSSYVKYVKSQEKVVTKKEYEQSREKWLKEKK
ASNEKAQKDLEKHVK

Nucleotide

• Length: 1968 bp

>NTDB_id=307427 DV947_RS06690 WP_116920313.1 1262729..1264696(-) (amiA) [Streptococcus thermophilus strain ST109]
ATGAAACTTAAAAAAATTCTTGGAATTACGGGTGTAGCCCTTCTAAGTGTTGGTATGTTAGCGGCTTGTTCTTCTAAGTC
AAGTACTAGTGGAACGACTTATAGTAATATTTATGGCTCAGATCCAGAAACCTTGGATTATATTACATCAATTATGGGAG
GGACAAAAGCTGTTTTGACTAATGGTGTAGATGGTCTTATGGAAGCGGACAAATATGGTAATCTTGTTCCATCAGTAGCT
GAAGACTGGTCTGTGTCTAAAGATGGTCTGACTTATACCTATAAAATTCGTAAAGGGATCAAATGGTATACTTCTGAAGG
TGAAGAGTACGCTAATGTTACTGCTAAGGACTTTGTAACGGGTCTTAAGCATGCCGCTGATGCCAAATCTGGAGCCCTTT
ATTTGGTACAAGATTCTATTGCTGGATTGTCTGACTACCTTTCTGGTGCCAATAAAGATTTCTCAAATGTTGGCGTTAAA
GCAATAGACGATTACACTCTGCAATACACACTAAAAAAACAGGAACCATACTGGAACTCTAAGACAACCTATGGTGTGCT
CTTCCCAGTTAACGAAGACTTTCTTAAGAACAAAGGGAAAGATTTTGGTAAGTCAACAGACCCAACCTCTATCCTATATA
ACGGTCCTTTCCTTCTTAAATCTTTGACAGCTAAATCATCTATCGAGTTGACTAAGAATGAGAATTATTGGGATAAGAAA
AATGTGCATTTTGACTCTATCAAATTCTCATATTCTGATGGATCGGATCTAGAATCAATAGAACGTAGTTTCTCTGACGG
CGCTCTCAGCATTGCACGTGTCTTCCCTACGAGTTCAAACTATGCATCGGTAGAAAAAAAATATAAAGATAATATTTTTT
ACACAGAACCAGGTGCATCTACATCGGTTATTGGTGTAAACATTGACCGCCAAAGCTATAAATTCTCAGCTAAGAAGACA
GATGCTGAAAAGACATCAACTAAGAAGGCTCTTCTAAACAAAGACTTTCGTCAATCAATCAACTTCGCTATTGATCGTAC
AGCCTATCAATCACAAGTAAACGGTAAAGATGGCGCAGCTCTTGCTCTACGTAACCTTTTTGTTCCTTATGATTTTGTCA
GTGCAGGCGATAAGACCTTTGGTGATCTTGTGACAGAAAAAATGAGCTCTTATGGTGATGAGTGGTCTGGTGTTAACTTT
GCAGATGGTCAAGAGGGTCTCTACAATGCTGAAAAAGCTAAGACTGAGTTTGCCAAAGCCAAAGAGGCTCTTCAAGGAGA
AGGGGTACAATTCCCAGTTCACTTAGACCTTCCAGTTGATCAAAGTTCTAAACTTAGTGTTGCTCAAGCTCAATCTTTGA
AACAAACCATTGAAAAATCATTGGGTAGCGAAAATGTTGTTATAGATATTAACCAAATGTCATCTGATGACATGAATAAT
GTGACTAGTAATGCGGCAAATGCTGCAGCAGAAGACTGGGATATTTCTAACGGTGTGGGTTGGGGTCCAGACTATCAAGA
TCCATCAACTTATCTTGATATCTTAAAAACAACATCAAGTGAAAATACCAAGATTTTCAATGGTTATGATGATCCAAACA
ATGCAGCAGCAGCTCAAGTAGGTCTTAAGAATTATGATGCCTTGCTTGATTCAGCAGCTTCAGAAACAACAGACATCAAC
GCTCGTTACGACCGTTATGCTCAAGCCCAAGCTTGGTTGGAAGATAGCTCTCTTATCATTCCATTGACTGTTAGTAACGG
TGCAGCACCAGTTATCTCACGTTTGACACCATTTACGGGTGCTAGCATACAGGTGGGTGATAAGGGCTCATCCTACGTCA
AATATGTGAAATCACAAGAAAAAGTAGTTACTAAGAAAGAATATGAACAAAGTCGTGAAAAATGGCTTAAAGAAAAGAAA
GCATCTAACGAAAAAGCTCAAAAAGATCTTGAGAAACACGTTAAATAA

Domains

Predicted by InterproScan.

• Bacterial extracellular solute-binding proteins, family 5 Middle (SBP_bac_5) (75-520)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiA	Streptococcus salivarius strain HSISS4	88.872	100	0.89
amiA3	Streptococcus thermophilus LMG 18311	86.454	100	0.867
amiA3	Streptococcus thermophilus LMD-9	86.454	100	0.867