Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   LR49_RS01395 Genome accession   NZ_CP031537
Coordinates   288387..288884 (-) Length   165 a.a.
NCBI ID   WP_001803317.1    Uniprot ID   A0A2I7Y542
Organism   Staphylococcus aureus strain WCH-SK2     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 283387..293884
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LR49_RS01370 (LR49_01370) gcvPB 283549..285021 (-) 1473 WP_000202189.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  LR49_RS01375 (LR49_01375) gcvPA 285014..286360 (-) 1347 WP_000019684.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  LR49_RS01380 (LR49_01380) gcvT 286380..287471 (-) 1092 WP_000093351.1 glycine cleavage system aminomethyltransferase GcvT -
  LR49_RS01385 (LR49_01385) - 287630..288154 (-) 525 WP_116100530.1 shikimate kinase -
  LR49_RS01390 (LR49_01390) - 288144..288290 (-) 147 WP_001789879.1 hypothetical protein -
  LR49_RS01395 (LR49_01395) comGF 288387..288884 (-) 498 WP_001803317.1 competence type IV pilus minor pilin ComGF Machinery gene
  LR49_RS01400 (LR49_01400) comGE 288802..289101 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  LR49_RS01405 (LR49_01405) comGD 289088..289534 (-) 447 WP_001790850.1 competence type IV pilus minor pilin ComGD Machinery gene
  LR49_RS01410 (LR49_01410) comGC 289512..289823 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  LR49_RS01415 (LR49_01415) comGB 289837..290907 (-) 1071 WP_000775708.1 competence type IV pilus assembly protein ComGB Machinery gene
  LR49_RS01420 (LR49_01420) comGA 290879..291853 (-) 975 WP_000697228.1 competence type IV pilus ATPase ComGA Machinery gene
  LR49_RS01425 (LR49_01425) - 291905..292528 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  LR49_RS01430 (LR49_01430) - 292525..292854 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  LR49_RS01435 (LR49_01435) - 292854..293840 (-) 987 WP_000161314.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19212.92 Da        Isoelectric Point: 10.2192

>NTDB_id=307294 LR49_RS01395 WP_001803317.1 288387..288884(-) (comGF) [Staphylococcus aureus strain WCH-SK2]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMFISITLLIVPDLIRLSKTFLIESRDLTTVDFEFFSRDI
LDDFKGVDRNDIEIRQHRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=307294 LR49_RS01395 WP_001803317.1 288387..288884(-) (comGF) [Staphylococcus aureus strain WCH-SK2]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGTTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGTAAAACTTTTCTAATTGAAAGTAGGGATTTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGATGATTTTAAAGGAGTAGATAGAAACGATATTGAAATTAGGCAACACCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATTAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(67-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2I7Y542

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus MW2

99.394

100

0.994
  comGF Staphylococcus aureus N315

95.758

100

0.958

Multiple sequence alignment