Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   DWG18_RS11060 Genome accession   NZ_CP031413
Coordinates   2280599..2281858 (+) Length   419 a.a.
NCBI ID   WP_115647241.1    Uniprot ID   A0A345ZLY8
Organism   Lysobacter sp. TY2-98     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2275599..2286858
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DWG18_RS11035 (DWG18_11035) - 2276315..2278054 (+) 1740 WP_115647236.1 diguanylate cyclase -
  DWG18_RS11040 (DWG18_11040) - 2278161..2279213 (+) 1053 WP_115647237.1 GAF domain-containing protein -
  DWG18_RS11045 (DWG18_11045) - 2279291..2279497 (-) 207 WP_115647238.1 cold-shock protein -
  DWG18_RS11050 (DWG18_11050) - 2279640..2280119 (-) 480 WP_115647239.1 hypothetical protein -
  DWG18_RS11055 (DWG18_11055) - 2280214..2280480 (+) 267 WP_115647240.1 DUF3297 family protein -
  DWG18_RS11060 (DWG18_11060) pilU 2280599..2281858 (+) 1260 WP_115647241.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DWG18_RS11065 (DWG18_11065) - 2281902..2282699 (+) 798 WP_115647242.1 MOSC N-terminal beta barrel domain-containing protein -
  DWG18_RS11070 (DWG18_11070) - 2282702..2283682 (-) 981 WP_205289343.1 NAD(P)H-quinone oxidoreductase -
  DWG18_RS11075 (DWG18_11075) - 2283738..2285348 (-) 1611 WP_115647243.1 diguanylate cyclase -
  DWG18_RS11080 (DWG18_11080) - 2285557..2286153 (-) 597 WP_115647244.1 YdeI/OmpD-associated family protein -
  DWG18_RS15655 (DWG18_11085) - 2286382..2286684 (+) 303 WP_115647245.1 KGG domain-containing protein -

Sequence

Protein


Download         Length: 419 a.a.        Molecular weight: 46774.45 Da        Isoelectric Point: 7.1863

>NTDB_id=306570 DWG18_RS11060 WP_115647241.1 2280599..2281858(+) (pilU) [Lysobacter sp. TY2-98]
MTQLTDDQARDYMHKLLAAMANAGGSDLFIAHDFPPSMKAHGQMTPLSPQKLTGAVTQQLARSLMNDKQRAEFAEQFECN
FAIAVPGVSRFRVNVFMQQGHVGMVLRTIAAEIPNFEKLRLPDVLKELVLAKRGLILLVGGTGSGKSTSLAAMLDHRNRT
TAGHIITVEDPVEYAHASQKSLVTHREVGVDTHSWHHALKNTLRQAPDVILIGEIRDAETMEHAIAFAETGHLCLSTLHA
NSASQTMERIINFFPEERRTQLLMDLSANLRAIVSQRLVRTEDGKGRAAAIEILINTPTIAEKIFKGEFNELKAIMNKSR
ELGMRTFDWALFELYNDGMISYDEAIRNADSANELRLNIKLKSRRGEPANAAALSLSLDAAPTAEDAETIRREELRRQQE
KRDQLELERLQREKAARPA

Nucleotide


Download         Length: 1260 bp        

>NTDB_id=306570 DWG18_RS11060 WP_115647241.1 2280599..2281858(+) (pilU) [Lysobacter sp. TY2-98]
ATGACCCAGCTCACCGACGACCAGGCTCGCGATTACATGCACAAGCTCCTGGCCGCCATGGCGAACGCCGGCGGCTCGGA
CCTGTTCATCGCGCACGATTTCCCGCCGAGCATGAAGGCGCACGGCCAGATGACGCCGCTGAGCCCGCAGAAGCTCACCG
GCGCCGTCACCCAGCAGCTCGCGCGTTCGTTGATGAACGACAAGCAGCGTGCGGAGTTCGCCGAGCAGTTCGAATGCAAT
TTCGCGATCGCCGTGCCCGGCGTGTCGCGCTTCCGCGTCAACGTGTTCATGCAGCAGGGCCATGTCGGCATGGTGCTGCG
CACGATCGCCGCGGAAATTCCGAACTTCGAAAAGTTGCGGCTGCCCGATGTGCTGAAGGAGCTGGTGCTCGCCAAGCGCG
GCCTGATCCTGCTCGTCGGCGGCACCGGTTCCGGCAAGTCGACCTCGCTCGCCGCGATGCTCGACCACCGCAACCGCACC
ACCGCCGGTCACATCATCACGGTGGAAGACCCGGTCGAATACGCGCACGCCTCGCAGAAGTCGCTGGTGACGCACCGCGA
GGTCGGCGTCGACACGCACAGCTGGCACCACGCGCTCAAGAACACATTGCGCCAGGCGCCCGACGTGATCCTGATCGGCG
AGATCCGCGATGCGGAGACCATGGAGCACGCGATCGCGTTCGCCGAAACCGGCCATCTCTGCCTGTCCACGCTGCATGCC
AACAGCGCGTCGCAGACGATGGAACGCATCATCAATTTCTTCCCGGAAGAACGCCGCACGCAGCTGCTGATGGATCTTTC
CGCCAACCTGCGTGCGATCGTGTCGCAGCGCCTGGTGCGCACCGAAGACGGCAAGGGCCGCGCCGCGGCGATCGAGATCC
TGATCAACACGCCGACGATCGCCGAGAAGATCTTCAAGGGCGAGTTCAACGAGCTCAAGGCGATCATGAACAAGTCGCGC
GAGCTGGGCATGCGCACATTCGACTGGGCGCTGTTCGAGCTCTACAACGACGGCATGATCAGCTACGACGAAGCGATCCG
GAATGCCGACTCGGCCAACGAGCTGCGTCTCAACATCAAGCTGAAGAGCCGTCGTGGCGAACCGGCGAATGCGGCGGCGC
TGTCGCTGTCGCTCGATGCGGCGCCGACGGCGGAAGACGCTGAAACAATCCGTCGCGAGGAACTGCGTCGCCAGCAGGAA
AAGCGCGACCAGCTGGAACTCGAGCGCCTGCAGCGCGAGAAGGCCGCGCGACCGGCCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(123-281)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A345ZLY8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

57.065

87.828

0.501
  pilU Acinetobacter baylyi ADP1

53.955

84.487

0.456
  pilU Vibrio cholerae strain A1552

49.171

86.396

0.425

Multiple sequence alignment