Detailed information    

insolico Bioinformatically predicted

Overview

Name   comL   Type   Machinery gene
Locus tag   EW997_RS21275 Genome accession   NZ_CP035879
Coordinates   4291715..4292452 (-) Length   245 a.a.
NCBI ID   WP_000197686.1    Uniprot ID   P0AC03
Organism   Escherichia coli strain DS26-1     
Function   DNA binding (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4286715..4297452
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EW997_RS21245 - 4286825..4287238 (-) 414 WP_074507809.1 DUF2799 domain-containing protein -
  EW997_RS21250 aroF 4287448..4288518 (+) 1071 WP_097307009.1 3-deoxy-7-phosphoheptulonate synthase AroF -
  EW997_RS21255 tyrA 4288529..4289650 (+) 1122 WP_000225221.1 bifunctional chorismate mutase/prephenate dehydrogenase -
  EW997_RS21260 pheA 4289693..4290853 (-) 1161 WP_000200100.1 bifunctional chorismate mutase/prephenate dehydratase -
  EW997_RS21265 pheL 4290952..4290999 (-) 48 WP_001700969.1 pheA operon leader peptide PheL -
  EW997_RS21270 raiA 4291103..4291444 (-) 342 WP_000178456.1 ribosome-associated translation inhibitor RaiA -
  EW997_RS21275 comL 4291715..4292452 (-) 738 WP_000197686.1 outer membrane protein assembly factor BamD Machinery gene
  EW997_RS21280 rluD 4292587..4293567 (+) 981 WP_000079100.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  EW997_RS21285 yfiH 4293564..4294295 (+) 732 WP_000040129.1 purine nucleoside phosphorylase YfiH -
  EW997_RS21290 clpC 4294425..4296998 (+) 2574 WP_001235102.1 ATP-dependent chaperone ClpB Regulator

Sequence

Protein


Download         Length: 245 a.a.        Molecular weight: 27829.40 Da        Isoelectric Point: 6.4874

>NTDB_id=306333 EW997_RS21275 WP_000197686.1 4291715..4292452(-) (comL) [Escherichia coli strain DS26-1]
MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQDGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYY
KNADLPLAQAAIDRFIRLNPTHPNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLMENAYRQMQMNAQAEKVAKIIAA
NSSNT

Nucleotide


Download         Length: 738 bp        

>NTDB_id=306333 EW997_RS21275 WP_000197686.1 4291715..4292452(-) (comL) [Escherichia coli strain DS26-1]
ATGACGCGCATGAAATATCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGT
ACCTGATAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGC
AACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATCTACGCCTACTAT
AAAAACGCCGATTTGCCGTTAGCACAGGCTGCCATCGATCGTTTTATTCGCCTTAACCCGACCCATCCGAATATCGATTA
TGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGCGTCGATCGTAGCG
ATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACC
ACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGTGGCCGAGTACTATACAGA
ACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATG
CGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCA
AACAGCAGCAATACATAA

Domains

Predicted by InterProScan.

Outer membrane lipoprotein (YfiO)

(28-236)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P0AC03

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comL Neisseria meningitidis MC58

38.525

99.592

0.384
  comL Neisseria gonorrhoeae MS11

37.705

99.592

0.376

Multiple sequence alignment