Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   DV160_RS04025 Genome accession   NZ_CP031336
Coordinates   654791..655651 (-) Length   286 a.a.
NCBI ID   WP_002220276.1    Uniprot ID   -
Organism   Neisseria meningitidis strain M21717     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 649791..660651
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DV160_RS03985 ispB 650279..651253 (+) 975 WP_002235439.1 octaprenyl diphosphate synthase -
  DV160_RS03990 - 651258..651704 (+) 447 WP_002220271.1 DUF441 domain-containing protein -
  DV160_RS04010 pilF 652194..653870 (+) 1677 WP_002221967.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DV160_RS04015 - 653955..654164 (-) 210 WP_002212333.1 DNA gyrase inhibitor YacG -
  DV160_RS04020 coaE 654157..654789 (-) 633 WP_002220275.1 dephospho-CoA kinase -
  DV160_RS04025 pilD 654791..655651 (-) 861 WP_002220276.1 prepilin peptidase Machinery gene
  DV160_RS04030 pilG 655725..656957 (-) 1233 WP_002220278.1 type 4 pilus assembly protein PilG Machinery gene
  DV160_RS04040 pgi 657467..659110 (+) 1644 WP_002220279.1 glucose-6-phosphate isomerase -
  DV160_RS04045 - 659080..659310 (+) 231 WP_002212338.1 hypothetical protein -
  DV160_RS04050 dapD 659320..660141 (+) 822 WP_079889349.1 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase -

Sequence

Protein


Download         Length: 286 a.a.        Molecular weight: 31359.40 Da        Isoelectric Point: 8.2213

>NTDB_id=306065 DV160_RS04025 WP_002220276.1 654791..655651(-) (pilD) [Neisseria meningitidis strain M21717]
MSDLSVLSPFAVPLAAVFGLLVGSFLNVVIYRVPVMMERGWTVFAKEYLNLPLTEEESRTFNLMKPDSCCPKCRVPIRAW
QNIPIVSYLLLRGKCASCQTKISIRYPLIELLTGVLFGLVAWQYGWSWITLGGLILTAFLISLTFIDADTQYLPDSMTLP
LIWLGLIFNLDGGFVPLQSAVLGAVAGYGSIWLLCAVYKLIKGEIGMGNGDFKLIAALGAWLGISALPVLIFVSSLIGLV
AAIVMRVAKGQHFAFGPALTVSGWIIFTANDSVWRAVNWWLTHPVL

Nucleotide


Download         Length: 861 bp        

>NTDB_id=306065 DV160_RS04025 WP_002220276.1 654791..655651(-) (pilD) [Neisseria meningitidis strain M21717]
ATGTCTGATTTGTCTGTATTGTCGCCGTTTGCCGTGCCTTTGGCAGCGGTGTTCGGGCTGCTGGTCGGCAGTTTCTTAAA
TGTCGTCATTTACCGCGTGCCGGTCATGATGGAACGCGGCTGGACGGTATTTGCCAAAGAATATTTAAACCTGCCGCTGA
CCGAAGAGGAAAGCCGTACCTTCAACCTGATGAAACCGGATTCCTGCTGTCCCAAATGCCGCGTGCCGATACGCGCGTGG
CAGAACATCCCGATTGTCAGCTACCTGCTCCTGCGCGGCAAATGCGCCTCCTGCCAAACCAAAATCAGCATACGCTATCC
CTTAATCGAACTGCTGACCGGCGTATTGTTCGGGCTGGTTGCCTGGCAATACGGCTGGTCTTGGATTACATTGGGCGGTT
TGATACTGACCGCGTTTCTGATTTCCCTGACCTTTATCGATGCGGACACCCAATACCTGCCCGACTCGATGACATTACCC
TTGATTTGGCTGGGGCTGATATTTAATTTGGACGGCGGCTTCGTGCCTTTGCAGTCTGCCGTTTTAGGTGCGGTTGCCGG
CTATGGTTCGATATGGCTTTTATGCGCGGTATATAAACTGATTAAAGGCGAAATCGGTATGGGCAACGGAGATTTCAAAC
TGATTGCCGCATTGGGCGCGTGGCTCGGCATATCCGCATTGCCCGTGCTGATTTTTGTTTCCTCGCTGATCGGTTTGGTC
GCGGCAATCGTTATGCGCGTCGCCAAGGGGCAGCATTTTGCCTTCGGCCCCGCACTGACAGTTTCGGGCTGGATAATTTT
TACGGCAAACGATTCCGTATGGCGGGCGGTCAACTGGTGGCTGACCCATCCGGTGCTGTAA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(17-124)

Type IV leader peptidase family (Peptidase_A24)

(134-242)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

96.14

99.65

0.958
  pilD Vibrio cholerae strain A1552

44.444

100

0.448
  pilD Vibrio campbellii strain DS40M4

44.928

96.503

0.434
  pilD Acinetobacter baumannii D1279779

46.97

92.308

0.434
  pilD Acinetobacter nosocomialis M2

46.97

92.308

0.434

Multiple sequence alignment