Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilK   Type   Machinery gene
Locus tag   DV163_RS03620 Genome accession   NZ_CP031330
Coordinates   608985..609578 (-) Length   197 a.a.
NCBI ID   WP_002244085.1    Uniprot ID   Q9JZV1
Organism   Neisseria meningitidis strain M21955     
Function   type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 594885..611807 608985..609578 within 0


Gene organization within MGE regions


Location: 594885..611807
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DV163_RS03500 - 594984..595409 (+) 426 WP_002219430.1 hypothetical protein -
  DV163_RS03505 - 595653..595853 (+) 201 WP_002219431.1 hypothetical protein -
  DV163_RS03510 - 595921..596280 (+) 360 WP_002219432.1 hypothetical protein -
  DV163_RS03515 - 596305..597159 (+) 855 WP_002219433.1 YfdQ family protein -
  DV163_RS03520 - 597229..597444 (+) 216 WP_002219434.1 hypothetical protein -
  DV163_RS03525 - 597479..597739 (+) 261 WP_002223443.1 hypothetical protein -
  DV163_RS03530 - 597736..598029 (+) 294 WP_002244087.1 hypothetical protein -
  DV163_RS03535 - 598032..598223 (+) 192 WP_002219435.1 type ISP restriction/modification enzyme -
  DV163_RS03540 - 598223..598363 (+) 141 WP_002225349.1 hypothetical protein -
  DV163_RS03545 - 598360..598569 (+) 210 WP_002220687.1 hypothetical protein -
  DV163_RS03550 - 598627..599544 (-) 918 WP_002219437.1 KilA-N domain-containing protein -
  DV163_RS03555 - 600413..600769 (-) 357 WP_002223441.1 DUF4760 domain-containing protein -
  DV163_RS03565 - 601317..601697 (-) 381 WP_002220683.1 type II toxin-antitoxin system PemK/MazF family toxin -
  DV163_RS03570 - 601810..602882 (-) 1073 Protein_589 integrase arm-type DNA-binding domain-containing protein -
  DV163_RS03585 yaaA 603411..604190 (-) 780 WP_002225351.1 peroxide stress protein YaaA -
  DV163_RS03590 dapC 604453..605640 (-) 1188 WP_002225352.1 succinyldiaminopimelate transaminase -
  DV163_RS03595 dut 605718..606170 (-) 453 WP_002225353.1 dUTP diphosphatase -
  DV163_RS03600 - 606370..606735 (+) 366 Protein_593 AzlC family ABC transporter permease -
  DV163_RS03615 pilX 608507..608980 (-) 474 WP_002225356.1 PilX family type IV pilin Machinery gene
  DV163_RS03620 pilK 608985..609578 (-) 594 WP_002244085.1 pilus assembly protein Machinery gene
  DV163_RS03625 pilJ 609557..610498 (-) 942 WP_002244084.1 PilW family protein Machinery gene
  DV163_RS03630 pilV 610495..611115 (-) 621 WP_002225360.1 type IV pilus modification protein PilV Machinery gene
  DV163_RS03635 pilH 611139..611807 (-) 669 WP_002230433.1 Tfp pilus assembly protein FimT/FimU Machinery gene

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 21699.65 Da        Isoelectric Point: 6.3911

>NTDB_id=305821 DV163_RS03620 WP_002244085.1 608985..609578(-) (pilK) [Neisseria meningitidis strain M21955]
MRKQNTLTGIPTSDGQRGFALFIVLMVMIVVAFLVVTAAQSYNTEQRISANESDRKLALSLAEAALREGELQVLDLEYDT
DSKVTFSENCGKGLCAAVNVRTNNDNEEAFDNIVVQGKPTVEAVKRSCPANSTDLCIDKKGMEYKKGTRSVSKMPRYIIE
YLGVKNGENVYRVTAKAWGKNANTVVVLQSYVSNNDE

Nucleotide


Download         Length: 594 bp        

>NTDB_id=305821 DV163_RS03620 WP_002244085.1 608985..609578(-) (pilK) [Neisseria meningitidis strain M21955]
ATGCGCAAACAGAACACTTTGACGGGAATCCCGACTTCTGACGGACAGAGGGGGTTTGCACTGTTTATCGTGCTGATGGT
GATGATCGTCGTGGCTTTTTTGGTTGTAACTGCCGCGCAGTCTTACAATACCGAGCAGCGGATCAGTGCCAACGAATCAG
ACAGGAAATTGGCTTTGTCTTTGGCCGAGGCGGCTTTGCGGGAAGGCGAACTTCAGGTTTTGGATTTGGAATATGATACG
GACAGTAAGGTTACATTTAGCGAAAACTGTGGAAAAGGTCTGTGTGCCGCAGTGAATGTGCGGACAAATAATGATAATGA
AGAGGCTTTTGACAATATCGTGGTGCAAGGCAAGCCCACCGTTGAGGCGGTGAAGCGTTCTTGCCCTGCAAATTCTACCG
ACCTGTGCATTGACAAGAAAGGGATGGAATATAAGAAAGGCACGAGAAGCGTCAGCAAAATGCCACGTTATATTATCGAA
TATTTGGGCGTGAAGAACGGAGAAAATGTTTATCGGGTTACTGCCAAGGCTTGGGGTAAGAATGCCAATACCGTGGTCGT
CCTTCAATCTTATGTAAGCAATAATGATGAGTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilX C-term (PilX)

(92-192)

PilX N-terminal (PilX_N)

(17-67)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9JZV1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilK Neisseria gonorrhoeae MS11

86.634

100

0.888

Multiple sequence alignment