Detailed information    

experimental Experimentally validated

Overview

Name   pilK   Type   Machinery gene
Locus tag   NGFG_RS02435 Genome accession   NC_022240
Coordinates   449247..449858 (+) Length   203 a.a.
NCBI ID   WP_003687918.1    Uniprot ID   -
Organism   Neisseria gonorrhoeae MS11     
Function   type IV pilus   
DNA binding and uptake

Function

Mutants lacking one of five newly discovered pilin-like proteins PilH–L were not transformable or were significantly reduced in transformation competence and were found not to localize ComP to the pili.


Genomic Context

Location: 444247..454858
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NGFG_RS02410 (NGFG_00603) - 444722..445303 (-) 582 WP_003687910.1 superoxide dismutase -
  NGFG_RS02415 (NGFG_00605) dnaB 445467..446873 (+) 1407 WP_003694974.1 replicative DNA helicase -
  NGFG_RS02420 (NGFG_00606) pilH 447028..447690 (+) 663 WP_003687912.1 GspH/FimT family pseudopilin Machinery gene
  NGFG_RS02425 (NGFG_00607) pilV/pilI 447722..448330 (+) 609 WP_003694976.1 type IV pilus modification protein PilV Regulator
  NGFG_RS02430 (NGFG_00608) pilJ 448327..449268 (+) 942 WP_003694978.1 PilW family protein Machinery gene
  NGFG_RS02435 (NGFG_00609) pilK 449247..449858 (+) 612 WP_003687918.1 pilus assembly PilX family protein Machinery gene
  NGFG_RS02440 (NGFG_00610) pilL 449860..450333 (+) 474 WP_025455870.1 PilX family type IV pilin Machinery gene
  NGFG_RS02445 (NGFG_00611) - 450403..450711 (-) 309 WP_010951048.1 AzlD family protein -
  NGFG_RS14980 - 450708..451415 (-) 708 Protein_464 AzlC family ABC transporter permease -
  NGFG_RS02455 (NGFG_00615) dut 451581..452033 (+) 453 WP_003687923.1 dUTP diphosphatase -
  NGFG_RS02460 (NGFG_00616) dapC 452111..453298 (+) 1188 WP_003687924.1 succinyldiaminopimelate transaminase -
  NGFG_RS02465 (NGFG_00617) yaaA 453609..454388 (+) 780 WP_003694987.1 peroxide stress protein YaaA -

Sequence

Protein


Download         Length: 203 a.a.        Molecular weight: 22153.94 Da        Isoelectric Point: 5.3780

>NTDB_id=1139 NGFG_RS02435 WP_003687918.1 449247..449858(+) (pilK) [Neisseria gonorrhoeae MS11]
MRKQNTLTGIPTSDGQRGSALFIVLMVMIVVAFLVVTAAQSYNTEQRISANESDRKLALSLAEAALREGEFQVLDLEYTA
DSKVTFSENCEKGLCTAVNVRTNNNGNEEVFGNIVVQGTPTVEAVKRSCPAKSGKNSTGLCIDNQGVEYEKGTGNVSKMP
RYIIEYLGEKNNQNIYRVTAKAWGKNANTVVVLQSYVGNNDEQ

Nucleotide


Download         Length: 612 bp        

>NTDB_id=1139 NGFG_RS02435 WP_003687918.1 449247..449858(+) (pilK) [Neisseria gonorrhoeae MS11]
ATGCGCAAACAGAACACTTTGACAGGAATCCCGACTTCTGACGGACAGAGGGGGTCCGCACTGTTTATCGTGCTGATGGT
GATGATAGTCGTGGCCTTTTTGGTTGTAACTGCCGCCCAGTCCTACAATACCGAACAGAGGATCAGTGCCAACGAATCAG
ACAGGAAATTGGCTTTGTCTTTAGCCGAGGCGGCTTTGAGGGAAGGCGAATTTCAGGTTTTGGATTTGGAATATACTGCG
GATAGTAAGGTTACATTTAGCGAAAACTGTGAAAAAGGCCTGTGTACCGCAGTGAATGTGCGGACAAATAATAATGGTAA
TGAAGAGGTTTTTGGCAATATCGTGGTGCAAGGCACGCCCACCGTTGAGGCGGTGAAGCGTTCTTGCCCTGCAAAGTCTG
GCAAAAATTCTACCGGCCTGTGCATTGACAATCAGGGAGTGGAATATGAGAAAGGTACGGGAAACGTCAGCAAAATGCCG
CGTTATATTATCGAATATTTGGGCGAGAAGAATAACCAAAATATTTACAGGGTTACTGCCAAGGCTTGGGGTAAGAATGC
CAATACCGTGGTCGTCCTGCAATCTTATGTAGGCAATAATGATGAGCAATAA

Domains

Predicted by InterProScan.

Type IV pilus assembly protein PilX C-term (PilX)

(92-198)

PilX N-terminal (PilX_N)

(17-67)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Hanne C Winther-Larsen et al. (2005) A conserved set of pilin-like molecules controls type IV pilus dynamics and organelle-associated functions in Neisseria gonorrhoeae. Molecular Microbiology 56(4):903-17. [PMID: 15853879]