Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   EXA02_RS13345 Genome accession   NZ_CP035841
Coordinates   2751283..2752779 (+) Length   498 a.a.
NCBI ID   WP_001040689.1    Uniprot ID   -
Organism   Escherichia coli strain TW10722     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2746283..2757779
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXA02_RS13315 tusA 2746530..2746775 (+) 246 WP_000130621.1 sulfurtransferase TusA -
  EXA02_RS13320 zntA 2746877..2749075 (-) 2199 WP_000106574.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  EXA02_RS13325 yhhN 2749149..2749775 (-) 627 WP_000964718.1 lysoplasmalogenase -
  EXA02_RS13330 yhhM 2749916..2750275 (+) 360 WP_000042873.1 DUF2500 domain-containing protein -
  EXA02_RS13335 yhhL 2750278..2750547 (-) 270 WP_001295207.1 DUF1145 family protein -
  EXA02_RS13340 rsmD 2750537..2751133 (-) 597 WP_000743193.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  EXA02_RS13345 pilA 2751283..2752779 (+) 1497 WP_001040689.1 signal recognition particle-docking protein FtsY Machinery gene
  EXA02_RS13350 ftsE 2752782..2753450 (+) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  EXA02_RS13355 ftsX 2753443..2754501 (+) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  EXA02_RS13360 rpoH 2754746..2755600 (+) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  EXA02_RS13365 livJ 2755872..2756975 (+) 1104 WP_001350438.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  EXA02_RS13370 ataR 2757125..2757391 (+) 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54685.47 Da        Isoelectric Point: 4.1468

>NTDB_id=305653 EXA02_RS13345 WP_001040689.1 2751283..2752779(+) (pilA) [Escherichia coli strain TW10722]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQTVVEEIVQAQEPVKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAEVVAQPEPVVEETPEPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
AAEAAEEAVIVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIAD
VGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQ
QGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKK
IVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIED
LRPFKADDFIEALFARED

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=305653 EXA02_RS13345 WP_001040689.1 2751283..2752779(+) (pilA) [Escherichia coli strain TW10722]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAAACGGTTGTAGAAGAAATCGTTCAGGCGCAAGAGCCTGTGAAGGCTTCTGAACAAGCCGTCGAAG
AGCAGCCGCAGGCGCATACTGAGGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTCACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAACCGGAACCGGTCGTAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAACGTGAAGAGCTGCCGTTGCCGGAAGACGTCAACGCCGAAGAGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATCACCGACGAAGAGCTGGAAGCACAGGCGCTG
GCTGCCGAAGCGGCAGAAGAAGCGGTGATAGTGGTTCCTCCGGCAGAAGAAGAGCAGCCGGTTGAAGAAATTGCCCAGGA
GCAGGAAAAACCGACCAAAGAAGGTTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCG
GATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTGGAAGAACAGCTGTTGATCGCCGAT
GTGGGCGTGGAAACCACACGTAAAATAATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGC
GCTCTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGC
CGTTCGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCGCGTCAGTTTGAGCAG
CAGGGTAAATCGGTGATGCTGGCGGCGGGCGATACTTTCCGTGCAGCAGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCG
CAACAATATTCCGGTGATTGCCCAGCATACCGGTGCGGATTCCGCCTCTGTTATCTTCGACGCCATTCAGGCAGCTAAAG
CGCGTAATATCGACGTCCTGATTGCCGATACCGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAA
ATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATTGATGCCAGTACCGGGCAGAA
TGCGGTAAGCCAGGCCAAACTGTTCCACGAAGCTGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGA
AAGGCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGAT
TTGCGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(294-494)

SRP54-type protein, helical bundle domain (SRP54_N)

(211-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.831

77.309

0.378

Multiple sequence alignment