Detailed information    

experimental Experimentally validated

Overview

Name   pilN   Type   Machinery gene
Locus tag   KZH42_RS10320 Genome accession   NZ_CP079941
Coordinates   2071702..2072301 (+) Length   199 a.a.
NCBI ID   WP_002214520.1    Uniprot ID   Q4W563
Organism   Neisseria meningitidis strain C311     
Function   assembly of type IV pilus   
DNA binding and uptake

Function

pilN mutant has a substantially reduced transformation frequency.


Genomic Context

Location: 2066702..2077301
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KZH42_RS10305 (KZH42_10300) yihA 2067286..2067915 (+) 630 WP_002248759.1 ribosome biogenesis GTP-binding protein YihA/YsxC -
  KZH42_RS10310 (KZH42_10305) - 2068036..2070432 (-) 2397 WP_002219786.1 penicillin-binding protein 1A -
  KZH42_RS10315 (KZH42_10310) pilM 2070584..2071699 (+) 1116 WP_002214517.1 type IV pilus biogenesis protein PilM -
  KZH42_RS10320 (KZH42_10315) pilN 2071702..2072301 (+) 600 WP_002214520.1 PilN domain-containing protein Machinery gene
  KZH42_RS10325 (KZH42_10320) - 2072302..2072949 (+) 648 WP_002217908.1 type 4a pilus biogenesis protein PilO -
  KZH42_RS10330 (KZH42_10325) pilP 2072967..2073512 (+) 546 WP_002214522.1 pilus assembly protein PilP Machinery gene
  KZH42_RS10335 (KZH42_10330) pilQ 2073531..2075840 (+) 2310 WP_002225652.1 type IV pilus secretin PilQ Machinery gene
  KZH42_RS10340 (KZH42_10335) - 2076742..2077254 (+) 513 WP_002222978.1 shikimate kinase -

Sequence

Protein


Download         Length: 199 a.a.        Molecular weight: 22257.48 Da        Isoelectric Point: 9.5493

>NTDB_id=1093 KZH42_RS10320 WP_002214520.1 2071702..2072301(+) (pilN) [Neisseria meningitidis strain C311]
MNNLIKINLLPYREEMNKRKQQQFKTLMYGAVLTGVAAVAATYLFIDNMINNQSERNTLLETSIAHLDTELSEIQKLKQE
KDAFLIKKNKIEELQLKRLQAAKILDSLNEAVPGSTYLTSLDAVTADSYRLSGRTSSDNRVAAMMRAMPNTGIFKQPELL
SIKKNNSHQEFTLQATLQPIVKAAESKENPASGNAQEAN

Nucleotide


Download         Length: 600 bp        

>NTDB_id=1093 KZH42_RS10320 WP_002214520.1 2071702..2072301(+) (pilN) [Neisseria meningitidis strain C311]
ATGAACAATTTAATCAAAATCAACCTCCTCCCCTACAGGGAAGAGATGAACAAGCGCAAACAGCAGCAGTTTAAAACGCT
GATGTACGGTGCCGTGCTGACGGGCGTTGCCGCCGTTGCGGCAACCTACCTGTTTATCGACAATATGATCAATAACCAGT
CGGAAAGAAACACGCTGCTGGAAACCTCCATCGCACACTTGGATACCGAGCTGTCGGAAATACAAAAGCTCAAACAGGAA
AAAGATGCCTTCCTGATTAAGAAAAACAAAATCGAGGAGCTCCAGCTCAAACGCCTCCAAGCCGCAAAAATCCTCGACAG
CCTGAATGAGGCCGTCCCCGGAAGCACCTACCTGACCTCGCTGGATGCCGTTACCGCCGACTCTTATCGGCTCAGCGGCA
GGACATCCAGCGACAACCGCGTTGCCGCCATGATGAGGGCGATGCCCAATACCGGCATATTCAAGCAACCCGAATTGTTA
AGCATCAAGAAAAACAATTCGCATCAAGAATTTACCCTTCAGGCAACATTACAACCCATCGTAAAGGCGGCCGAATCCAA
AGAGAATCCGGCTTCGGGAAACGCACAGGAGGCAAACTGA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(104-177)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q4W563

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Yao-Hui Sun et al. (2005) Identification and characterization of genes required for competence in Neisseria meningitidis. Journal of Bacteriology 187(9):3273-6. [PMID: 15838056]