Detailed information    

insolico Bioinformatically predicted

Overview

Name   crp   Type   Regulator
Locus tag   EW646_RS20500 Genome accession   NZ_CP035770
Coordinates   4106401..4107033 (+) Length   210 a.a.
NCBI ID   WP_000242755.1    Uniprot ID   A0A1C4FZU4
Organism   Escherichia coli O145 strain RM8988-C1     
Function   promote expression of competence genes (predicted from homology)   
Competence regulation

Genomic Context

Location: 4101401..4112033
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EW646_RS20475 (EW646_21000) yheS 4101570..4103483 (+) 1914 WP_000634798.1 ABC transporter ATP-binding protein -
  EW646_RS20480 (EW646_21005) yheT 4103483..4104505 (+) 1023 WP_000057365.1 hydrolase -
  EW646_RS20485 (EW646_21010) yheU 4104499..4104717 (+) 219 WP_000907085.1 YheU family protein -
  EW646_RS20490 (EW646_21015) prkB 4104771..4105640 (+) 870 WP_001274680.1 phosphoribulokinase -
  EW646_RS20495 (EW646_21020) yhfA 4105695..4106099 (-) 405 WP_001148912.1 OsmC family protein -
  EW646_RS20500 (EW646_21025) crp 4106401..4107033 (+) 633 WP_000242755.1 cAMP-activated global transcriptional regulator CRP Regulator
  EW646_RS20505 (EW646_21030) yhfK 4107084..4109174 (+) 2091 WP_001400404.1 YccS/YhfK family putative transporter -
  EW646_RS20510 (EW646_21035) argD 4109241..4110461 (-) 1221 WP_000963777.1 bifunctional acetylornithine/succinyldiaminopimelate transaminase -
  EW646_RS20515 (EW646_21040) pabA 4110547..4111110 (-) 564 WP_000601869.1 aminodeoxychorismate synthase component 2 -
  EW646_RS20520 (EW646_21045) fic 4111142..4111744 (-) 603 WP_001280641.1 putative adenosine monophosphate-protein transferase Fic -
  EW646_RS20525 (EW646_21050) yhfG 4111734..4111901 (-) 168 WP_000736862.1 YhfG family protein -

Sequence

Protein


Download         Length: 210 a.a.        Molecular weight: 23640.43 Da        Isoelectric Point: 8.2710

>NTDB_id=304834 EW646_RS20500 WP_000242755.1 4106401..4107033(+) (crp) [Escherichia coli O145 strain RM8988-C1]
MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIVKGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEG
QERSAWVRAKTACEVAEISYKKFRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR

Nucleotide


Download         Length: 633 bp        

>NTDB_id=304834 EW646_RS20500 WP_000242755.1 4106401..4107033(+) (crp) [Escherichia coli O145 strain RM8988-C1]
ATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAA
GAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAG
ACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGC
CAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGAT
TCAGGTAAACCCGGACATTCTGATGCGTCTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCA
ACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTCAATCTGGCAAAACAACCAGACGCTATGACTCAC
CCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATCGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCAT
TCTGAAGATGCTTGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAA

Domains

Predicted by InterProScan.

Cyclic nucleotide-binding domain (cNMP_binding)

(23-109)

Crp-like helix-turn-helix domain (HTH_Crp_2)

(142-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1C4FZU4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  crp Vibrio cholerae strain A1552

95.714

100

0.957
  crp Haemophilus influenzae Rd KW20

77.941

97.143

0.757
  crp Acinetobacter baumannii D1279779

47.343

98.571

0.467

Multiple sequence alignment