Detailed information NTG0030460

Bioinformatically predicted

Overview

• Name: pilJ
• Type: Machinery gene
• Locus tag: CCE_RS02135
• Genome accession: NC_010546
• Coordinates: 450239..452986 (-)
• Length: 915 a.a.
• NCBI ID: WP_009546531.1
• Uniprot ID: B1WNF3
• Organism: Crocosphaera subtropica ATCC 51142
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 445239..457986

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CCE_RS02115 (cce_0439)	-	446169..447311 (-)	1143	WP_009546527.1	SUMF1/EgtB/PvdO family nonheme iron enzyme	-
CCE_RS02120 (cce_0440)	-	447494..448441 (+)	948	WP_009546528.1	undecaprenyl-diphosphate phosphatase	-
CCE_RS02125 (cce_0441)	psb29	448536..449264 (-)	729	WP_009546529.1	photosystem II biogenesis protein Psp29	-
CCE_RS02130 (cce_0443)	-	449436..450134 (-)	699	WP_009546530.1	DUF2993 domain-containing protein	-
CCE_RS02135 (cce_0444)	pilJ	450239..452986 (-)	2748	WP_009546531.1	methyl-accepting chemotaxis protein	Machinery gene
CCE_RS02140 (cce_0445)	-	453091..453621 (-)	531	WP_009546532.1	chemotaxis protein CheW	-
CCE_RS02145 (cce_0446)	pilH	453652..454017 (-)	366	WP_009546533.1	response regulator	Machinery gene
CCE_RS02150 (cce_0448)	-	454271..455503 (-)	1233	WP_009546534.1	response regulator	-
CCE_RS02155 (cce_0449)	hmpF	456372..457979 (+)	1608	WP_009546535.1	pilus motility taxis protein HmpF	-

Sequence

Protein

• Length: 915 a.a.
• Molecular weight: 100036.95 Da
• Isoelectric Point: 4.1459

>NTDB_id=30460 CCE_RS02135 WP_009546531.1 450239..452986(-) (pilJ) [Crocosphaera subtropica ATCC 51142]
MASGTDYAKLYNEAYVAYGQGNFEEAAALMEPMAEAFPEDPDVLLLRGHIYFSLGRYETACQQYQSVIGHTDRPDLVDCA
KQGLAQAQQLLAQSGDNHRSNELAEPSEIASESVDRKEQWSEPTDSSGDWESEDFELDGLDWDSAIFDDDDLGEPTIGHQ
VSQGQPFDNNHHPPEQRPANFEMEVDPFTTLSEVKPFDSQTDIPKTNTSDKENWTDFVDHEFSDFSFGDEQGLETEAHTG
GEIAGDEDNTFVVSSSLNPPSLDNRGSTTSPEEPSSDWSNYPAQDDPTIQATPRMMPSNPAQEQNQVLDKFDAFQEEALE
DLSNFDFTEMSDQLPDSDLFSQASEDLSGSLGLQTGDLLSEQGSQTVNWVKSDDMAHSSLSNSETKVIKPTVEAKQGYLG
WFHNAPLNLKPWMAAGSTGIVSFVAILLVSGFAWLISPQAESTKVVDKKSTADKVETSENNTNPLQATGQASQQSPSTPN
NKKQAKSGDPILLLALTGGLVGFGGTLFFGLIASYHLKRTIADLQTQFGSMYGGDYNVKATIYSEDELGQLSYNFNQLSR
VILTTTSEAQQRAAEMEQAREELQRQVIRLLDDVEGAARGDLTVEAEVTADVLGAVADAFNLTIHNLREIVAQVKRTAKQ
VNKSSTDSELFARNNSRDALRMAEELAVTLNSVQVMTESIQRVAENAREAEEVAHTSSVTALKGGEAVERTVAGILQIRE
TVSETTRKVKRLAEASQEISKIVALISQIASRTNLLALNASIQAARAGEAGRGFAIVADEVRQLADRSAKSLKEIEQIVL
QIQSETGSVMTAMEEGIQQVIDVTDKSEQAKRSLEDIIDVSNHINTLVRSITGDTIQQQENSKAVSQVMQSVELTAQETS
QESQRVAGSLQNLVGISRDLLASVERFHIEEAQKS

Nucleotide

• Length: 2748 bp

>NTDB_id=30460 CCE_RS02135 WP_009546531.1 450239..452986(-) (pilJ) [Crocosphaera subtropica ATCC 51142]
ATGGCATCAGGGACAGACTACGCAAAATTATATAATGAAGCTTATGTCGCTTACGGACAGGGAAATTTTGAAGAGGCCGC
TGCCCTCATGGAACCAATGGCTGAGGCTTTTCCCGAAGATCCTGATGTTCTGTTATTACGGGGTCATATTTATTTTAGCT
TGGGAAGATACGAAACCGCTTGTCAACAATATCAATCGGTCATTGGCCACACTGATCGCCCAGATTTAGTAGATTGCGCT
AAGCAAGGATTAGCACAAGCCCAACAACTGCTTGCTCAATCAGGGGATAATCATCGAAGCAACGAACTAGCGGAGCCATC
TGAGATAGCATCTGAGTCAGTTGACAGGAAAGAACAGTGGTCTGAGCCGACAGATTCATCAGGAGATTGGGAGTCTGAAG
ATTTTGAACTCGATGGACTAGACTGGGATAGTGCTATTTTTGATGACGATGATTTAGGAGAACCGACTATTGGTCACCAA
GTATCTCAAGGGCAACCTTTTGATAATAATCATCATCCACCAGAGCAAAGACCAGCCAACTTTGAAATGGAGGTTGATCC
CTTTACCACCCTAAGTGAAGTAAAGCCTTTTGACTCTCAAACGGATATCCCCAAAACAAACACCTCAGATAAAGAGAATT
GGACTGATTTTGTTGACCATGAGTTCTCTGATTTTTCTTTTGGTGACGAGCAAGGGCTAGAAACTGAAGCACATACGGGA
GGCGAAATAGCAGGGGATGAAGACAATACCTTTGTTGTTTCCTCTAGTCTTAATCCTCCTTCATTGGATAATCGTGGCTC
TACAACCTCACCAGAAGAACCCTCTTCTGATTGGTCTAATTATCCTGCTCAGGATGATCCTACCATTCAAGCCACGCCCA
GGATGATGCCATCCAATCCAGCACAAGAACAAAACCAAGTCTTAGATAAGTTTGATGCTTTTCAGGAAGAGGCATTAGAG
GATTTATCCAATTTTGATTTTACCGAGATGAGCGATCAGTTACCTGATTCTGATCTGTTCAGTCAAGCCAGCGAGGATCT
CAGTGGTAGTCTCGGTTTACAAACGGGGGATCTTTTATCTGAACAAGGATCACAAACTGTTAATTGGGTAAAATCTGATG
ACATGGCCCATAGTTCTTTGTCCAATAGTGAGACTAAAGTCATCAAACCCACTGTTGAAGCAAAACAAGGCTATTTAGGT
TGGTTCCATAATGCACCTTTAAACTTAAAACCGTGGATGGCCGCCGGAAGCACAGGTATCGTTTCTTTTGTGGCTATTCT
GCTGGTGAGTGGCTTCGCTTGGTTAATTTCTCCTCAAGCAGAATCGACTAAAGTGGTGGATAAAAAATCCACAGCAGACA
AGGTAGAAACGTCTGAGAACAATACGAACCCGTTACAAGCAACTGGACAGGCCTCTCAACAATCTCCATCTACTCCAAAC
AACAAGAAACAAGCTAAATCTGGTGACCCTATCTTGTTACTAGCCTTAACAGGGGGTTTAGTCGGATTTGGGGGAACACT
GTTTTTTGGCCTGATCGCTTCCTACCACCTAAAACGCACCATTGCGGATCTCCAGACTCAGTTTGGTTCAATGTATGGGG
GTGACTACAATGTAAAGGCCACCATTTACTCCGAAGATGAATTAGGGCAATTATCCTATAATTTTAACCAATTATCCCGT
GTTATCCTCACCACCACCAGCGAAGCCCAACAACGGGCTGCAGAAATGGAACAAGCGAGAGAAGAGTTGCAACGGCAAGT
GATTCGCCTTTTGGATGATGTAGAGGGGGCTGCTAGGGGAGATTTAACTGTAGAAGCAGAGGTAACGGCTGACGTATTAG
GGGCGGTGGCAGATGCCTTTAACTTAACCATCCACAATCTACGGGAAATTGTCGCCCAGGTAAAACGAACTGCAAAACAG
GTTAATAAAAGTTCAACGGATAGTGAATTATTTGCTCGCAATAATTCCCGTGATGCTCTGCGAATGGCAGAAGAATTAGC
CGTCACCTTAAATTCGGTACAGGTGATGACTGAATCGATTCAACGGGTGGCAGAAAACGCCCGTGAAGCTGAAGAAGTTG
CCCACACCTCCTCCGTAACCGCTCTCAAAGGGGGTGAAGCGGTAGAGCGAACAGTAGCTGGTATTCTGCAAATTCGAGAA
ACGGTATCAGAAACCACTCGTAAAGTAAAACGGTTAGCGGAAGCATCTCAAGAAATCTCAAAGATTGTAGCGTTAATTTC
TCAAATTGCTTCAAGAACGAACTTATTGGCTCTTAATGCGTCAATTCAAGCAGCTAGAGCAGGAGAAGCAGGACGAGGCT
TTGCCATCGTAGCTGATGAAGTTCGTCAGTTAGCTGATCGTTCGGCAAAATCATTGAAAGAAATTGAGCAAATCGTACTA
CAAATTCAAAGTGAAACCGGTTCGGTAATGACAGCAATGGAAGAAGGGATTCAGCAGGTAATCGATGTAACGGATAAATC
AGAACAGGCGAAACGTTCTTTAGAAGATATTATTGACGTATCCAATCACATTAATACCTTAGTGCGATCCATTACAGGGG
ATACCATTCAACAACAGGAAAATTCTAAGGCAGTGAGTCAAGTTATGCAGTCTGTGGAATTAACTGCACAAGAAACATCC
CAAGAATCTCAACGGGTAGCAGGATCGTTACAAAATCTAGTAGGAATTTCCCGTGATTTATTGGCTTCTGTGGAACGTTT
CCATATCGAAGAGGCTCAGAAATCTTAA

Domains

Predicted by InterproScan.

• Tetratricopeptide repeat (TPR_16) (11-70)

• Methyl-accepting chemotaxis protein (MCP) signalling domain (MCPsignal) (692-875)

• HAMP domain (HAMP) (519-560)

3D structure

• AlphaFold DB: B1WNF3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilJ	Synechocystis sp. PCC 6803	53.476	100	0.546