Detailed information NTG0304535

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: EWS90_RS26610
• Genome accession: NZ_CP035739
• Coordinates: 5501867..5502739 (-)
• Length: 290 a.a.
• NCBI ID: WP_003107301.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain 1334/14
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5496867..5507739

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EWS90_RS26565 (EWS90_26565)	-	5497728..5497898 (-)	171	WP_003094672.1	DUF3094 family protein	-
EWS90_RS26570 (EWS90_26570)	-	5497924..5498373 (-)	450	WP_021264127.1	MOSC domain-containing protein	-
EWS90_RS26575 (EWS90_26575)	-	5498370..5498999 (-)	630	WP_003094668.1	DUF1780 domain-containing protein	-
EWS90_RS26580 (EWS90_26580)	-	5499132..5499557 (+)	426	WP_003103865.1	GNAT family N-acetyltransferase	-
EWS90_RS26585 (EWS90_26585)	-	5499554..5500024 (+)	471	WP_003094664.1	hypothetical protein	-
EWS90_RS26590 (EWS90_26590)	-	5500021..5500710 (+)	690	WP_003103868.1	energy-coupling factor ABC transporter permease	-
EWS90_RS26595 (EWS90_26595)	-	5500816..5501025 (+)	210	WP_003094660.1	hypothetical protein	-
EWS90_RS26600 (EWS90_26600)	yacG	5501062..5501262 (-)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-
EWS90_RS26605 (EWS90_26605)	coaE	5501259..5501870 (-)	612	WP_003112838.1	dephospho-CoA kinase	-
EWS90_RS26610 (EWS90_26610)	pilD	5501867..5502739 (-)	873	WP_003107301.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
EWS90_RS26615 (EWS90_26615)	pilC	5502740..5503957 (-)	1218	WP_003107299.1	type II secretion system F family protein	Machinery gene
EWS90_RS26620 (EWS90_26620)	pilB	5503961..5505661 (-)	1701	WP_003122078.1	type IV-A pilus assembly ATPase PilB	Machinery gene
EWS90_RS26625 (EWS90_26625)	pilA/pilAI	5505892..5506356 (+)	465	WP_023129290.1	pilin	Machinery gene

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 31873.90 Da
• Isoelectric Point: 7.7068

>NTDB_id=304535 EWS90_RS26610 WP_003107301.1 5501867..5502739(-) (pilD) [Pseudomonas aeruginosa strain 1334/14]
MTLLDYLASHPLAFVLCTILLGLLVGSFLNVVVHRLPKMMERNWKAEAREALGLEPEPKQATYNLVLPNSACPRCGHEIR
PWENIPLVSYLALGGKCSSCKAAIGKRYPLVELATALLSGYVAWHFGFTWQAGAMLLLTWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIANHFGLFASLDDALFGAVFGYLSLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AILGVIMLRLRNAESGTPIPFGPYLAIAGWIALLWGDQITRTYLQFAGFK

Nucleotide

• Length: 873 bp

>NTDB_id=304535 EWS90_RS26610 WP_003107301.1 5501867..5502739(-) (pilD) [Pseudomonas aeruginosa strain 1334/14]
ATGACCCTCCTCGACTACCTGGCCAGCCATCCGCTGGCCTTTGTCTTGTGCACCATCCTCCTCGGCCTGCTGGTCGGCAG
CTTCCTCAACGTCGTCGTGCATCGCCTGCCGAAGATGATGGAGCGCAACTGGAAGGCCGAAGCCCGCGAAGCGCTCGGGC
TGGAGCCGGAACCCAAGCAGGCGACCTACAACCTGGTGCTGCCGAATTCGGCGTGCCCGCGCTGCGGCCACGAGATCCGC
CCCTGGGAAAACATCCCGCTGGTCAGCTACCTGGCGCTGGGCGGCAAGTGCTCGTCCTGCAAGGCCGCCATCGGCAAACG
TTACCCCCTGGTGGAACTGGCCACCGCGCTGCTTTCCGGCTACGTCGCCTGGCATTTCGGCTTCACCTGGCAGGCGGGCG
CGATGCTCCTGCTGACCTGGGGCCTGCTGGCGATGAGCCTGATCGATGCCGATCACCAACTGCTGCCGGACGTGCTGGTG
CTGCCGCTGCTCTGGCTGGGCCTGATCGCCAACCATTTCGGCCTCTTCGCCAGCCTCGACGATGCGCTGTTCGGCGCGGT
GTTCGGCTACCTGAGCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAGGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCCATGCTCGGTGCCTGGGGCGGCTGGCAGATCCTGCCGCTGACCATCCTGCTGTCGTCGCTGGTCGGG
GCGATCCTCGGGGTGATCATGCTGCGCCTGCGCAACGCCGAAAGCGGCACGCCGATCCCCTTCGGTCCCTATCTGGCGAT
TGCCGGGTGGATTGCTTTGCTCTGGGGTGATCAAATAACCCGGACCTATCTGCAATTCGCCGGATTCAAATGA

Domains

Predicted by InterProScan.

• Type IV leader peptidase family (Peptidase_A24) (137-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	55.357	96.552	0.534
pilD	Vibrio campbellii strain DS40M4	52.65	97.586	0.514
pilD	Acinetobacter nosocomialis M2	50.53	97.586	0.493
pilD	Acinetobacter baumannii D1279779	50.53	97.586	0.493
pilD	Neisseria gonorrhoeae MS11	48.921	95.862	0.469