Detailed information NTG0300436

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: DQ228_RS07185
• Genome accession: NZ_CP030250
• Coordinates: 1403074..1404159 (-)
• Length: 361 a.a.
• NCBI ID: WP_084828981.1
• Uniprot ID: A0A0E2Q0D9
• Organism: Streptococcus thermophilus strain CS20
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 1398074..1409159

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DQ228_RS07155	-	1398438..1399253 (-)	816	WP_011226298.1	Cof-type HAD-IIB family hydrolase	-
DQ228_RS07160	-	1399246..1400040 (-)	795	WP_011681412.1	HAD-IIB family hydrolase	-
DQ228_RS07165	-	1400225..1400602 (+)	378	WP_011681413.1	GntR family transcriptional regulator	-
DQ228_RS07170	-	1400607..1401305 (+)	699	WP_023909821.1	ABC transporter ATP-binding protein	-
DQ228_RS07175	-	1401317..1402102 (+)	786	WP_220016107.1	ABC transporter permease	-
DQ228_RS07180	amiF	1402152..1403081 (-)	930	WP_082245810.1	ATP-binding cassette domain-containing protein	Regulator
DQ228_RS07185	amiE	1403074..1404159 (-)	1086	WP_084828981.1	ABC transporter ATP-binding protein	Regulator
DQ228_RS07190	amiD	1404169..1405095 (-)	927	WP_084828982.1	oligopeptide ABC transporter permease OppC	Regulator
DQ228_RS07195	amiC	1405095..1406588 (-)	1494	WP_064355564.1	ABC transporter permease	Regulator
DQ228_RS07200	amiA3	1406649..1408619 (-)	1971	WP_064355565.1	peptide ABC transporter substrate-binding protein	Regulator

Sequence

Protein

• Length: 361 a.a.
• Molecular weight: 39806.59 Da
• Isoelectric Point: 4.8014

>NTDB_id=300436 DQ228_RS07185 WP_084828981.1 1403074..1404159(-) (amiE) [Streptococcus thermophilus strain CS20]
MTENKNVILSARDIVVEFDVRDRVLTAIRGVSLDLVEGEVLALVGESGSGKSVLTKTFTGMLEENGRVASGSIDYRGKDL
TKFKSHQDWAAIRGAKIATIFQDPMTSLNPIKTIGSQIIEVIVKHQGKTAKEAKKMAIDYMDKVGIPDAEKRFNEYPFQY
SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLKEEYGFSVIFITHDLGVVASIADKVAVMYAGEIIE
YATVEEIFYEPCHPYTWSLLSSLPQLAGDNGKLFSIPGTPPSLYTPVVGDAFALRSDYALQIDFEEKAPQFQVSDTHWAK
TWLLHEDAPKVDKPAVIQNLHEKILANMGFAHLGDEEEGNA

Nucleotide

• Length: 1086 bp

>NTDB_id=300436 DQ228_RS07185 WP_084828981.1 1403074..1404159(-) (amiE) [Streptococcus thermophilus strain CS20]
ATGACAGAAAATAAAAATGTAATATTATCGGCTCGCGATATCGTCGTGGAATTTGACGTTCGTGACCGTGTTTTGACAGC
TATTCGAGGGGTTTCTCTTGATTTAGTTGAAGGTGAAGTTTTAGCCTTGGTTGGTGAGTCTGGTTCAGGAAAATCAGTCT
TGACTAAAACTTTTACAGGGATGCTGGAAGAAAATGGTCGTGTAGCTAGCGGATCTATCGACTACCGTGGCAAAGACTTG
ACTAAATTTAAGAGTCACCAAGATTGGGCAGCTATCCGTGGAGCTAAGATTGCAACTATTTTCCAAGATCCAATGACCAG
TCTTAACCCAATTAAGACTATCGGAAGTCAAATTATTGAAGTTATTGTTAAGCACCAAGGGAAAACAGCCAAGGAAGCTA
AAAAAATGGCAATTGATTACATGGACAAGGTTGGTATTCCAGATGCTGAAAAACGTTTCAATGAATATCCTTTCCAATAC
TCTGGTGGGATGCGTCAACGTATCGTTATTGCCATTGCCTTGGCTTGTCGTCCTGATGTCCTTATCTGTGACGAACCAAC
AACTGCCCTTGATGTGACCATTCAAGCTCAAATCATTGACCTCTTGAAATCACTCAAAGAGGAGTATGGTTTCTCGGTCA
TTTTCATTACCCATGACCTTGGCGTCGTAGCAAGTATCGCGGACAAGGTTGCTGTCATGTATGCTGGCGAAATCATTGAA
TACGCAACTGTTGAGGAAATTTTCTATGAGCCTTGTCATCCATACACGTGGAGCTTGCTTTCAAGTCTGCCACAATTGGC
TGGTGATAATGGAAAACTCTTCTCAATTCCAGGGACGCCACCATCACTTTATACACCAGTTGTTGGGGATGCCTTTGCCT
TGCGTTCAGATTATGCTTTGCAGATTGATTTTGAGGAAAAAGCACCACAATTCCAAGTTTCAGATACTCACTGGGCTAAG
ACCTGGCTCTTACATGAGGATGCGCCAAAAGTTGATAAACCTGCGGTTATCCAAAATCTACATGAAAAAATCCTAGCCAA
TATGGGATTTGCACATTTAGGAGATGAGGAGGAAGGCAATGCCTGA

Domains

Predicted by InterproScan.

• ABC transporter (ABC_tran) (29-187)

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (239-300)

3D structure

• AlphaFold DB: A0A0E2Q0D9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus thermophilus LMG 18311	99.723	100	0.997
amiE	Streptococcus thermophilus LMD-9	99.723	100	0.997
amiE	Streptococcus salivarius strain HSISS4	96.676	100	0.967
oppD	Streptococcus mutans UA159	54.441	96.676	0.526