Detailed information NTG0299725

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: BVDSYZ_RS13875
• Genome accession: NZ_CP030150
• Coordinates: 2780882..2781496 (-)
• Length: 204 a.a.
• NCBI ID: WP_015240245.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain DSYZ
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2775882..2786496

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BVDSYZ_RS13850 (BVDSYZ_13850)	rpsT	2776185..2776451 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
BVDSYZ_RS13855 (BVDSYZ_13855)	holA	2776468..2777509 (-)	1042	Protein_2676	DNA polymerase III subunit delta	-
BVDSYZ_RS21935	-	2777549..2777700 (-)	152	Protein_2677	hypothetical protein	-
BVDSYZ_RS13860 (BVDSYZ_13860)	-	2777741..2777875 (+)	135	WP_003152870.1	YqzM family protein	-
BVDSYZ_RS13865 (BVDSYZ_13865)	comEC	2777894..2780245 (-)	2352	WP_062623529.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
BVDSYZ_RS13870 (BVDSYZ_13870)	-	2780246..2780815 (-)	570	WP_003152868.1	ComE operon protein 2	-
BVDSYZ_RS13875 (BVDSYZ_13875)	comEA	2780882..2781496 (-)	615	WP_015240245.1	helix-hairpin-helix domain-containing protein	Machinery gene
BVDSYZ_RS13880 (BVDSYZ_13880)	comER	2781555..2782376 (+)	822	WP_039254409.1	late competence protein ComER	-
BVDSYZ_RS13885 (BVDSYZ_13885)	-	2782445..2783182 (-)	738	WP_015417856.1	class I SAM-dependent methyltransferase	-
BVDSYZ_RS13890 (BVDSYZ_13890)	rsfS	2783179..2783535 (-)	357	WP_007408260.1	ribosome silencing factor	-
BVDSYZ_RS13895 (BVDSYZ_13895)	yqeK	2783553..2784113 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
BVDSYZ_RS13900 (BVDSYZ_13900)	-	2784103..2784672 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
BVDSYZ_RS13905 (BVDSYZ_13905)	yhbY	2784683..2784973 (-)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
BVDSYZ_RS13910 (BVDSYZ_13910)	aroE	2784967..2785809 (-)	843	WP_039254404.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22209.32 Da
• Isoelectric Point: 5.5211

>NTDB_id=299725 BVDSYZ_RS13875 WP_015240245.1 2780882..2781496(-) (comEA) [Bacillus velezensis strain DSYZ]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=299725 BVDSYZ_RS13875 WP_015240245.1 2780882..2781496(-) (comEA) [Bacillus velezensis strain DSYZ]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363