Detailed information    

insolico Bioinformatically predicted

Overview

Name   phrF   Type   Regulator
Locus tag   DOU34_RS16715 Genome accession   NZ_CP030097
Coordinates   3368354..3368473 (+) Length   39 a.a.
NCBI ID   WP_007407639.1    Uniprot ID   A3KLD5
Organism   Bacillus amyloliquefaciens strain SH-B74     
Function   antagonize RapF (predicted from homology)   
Competence regulation

Genomic Context

Location: 3363354..3373473
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DOU34_RS16690 - 3363704..3364216 (+) 513 Protein_3251 bacteriocin biosynthesis protein AlbG -
  DOU34_RS16695 - 3364223..3365599 (-) 1377 WP_061047017.1 YncE family protein -
  DOU34_RS16700 - 3365639..3366994 (-) 1356 WP_061047016.1 YncE family protein -
  DOU34_RS16710 rapF 3367225..3368370 (+) 1146 WP_007407638.1 tetratricopeptide repeat protein Regulator
  DOU34_RS16715 phrF 3368354..3368473 (+) 120 WP_007407639.1 PhrC/PhrF family phosphatase-inhibitory pheromone Regulator
  DOU34_RS16720 - 3368671..3368874 (+) 204 WP_007407640.1 helix-turn-helix transcriptional regulator -
  DOU34_RS16725 - 3368888..3369091 (+) 204 WP_007407641.1 hypothetical protein -
  DOU34_RS16730 - 3369126..3369311 (+) 186 WP_061047015.1 DUF4177 domain-containing protein -
  DOU34_RS16735 - 3369497..3369778 (+) 282 WP_007407643.1 hypothetical protein -
  DOU34_RS16740 - 3369822..3370334 (+) 513 WP_061047014.1 stage II sporulation protein M -
  DOU34_RS16750 - 3370925..3371143 (-) 219 WP_003151056.1 LuxR C-terminal-related transcriptional regulator -
  DOU34_RS16755 speB 3371311..3372183 (-) 873 WP_007407646.1 agmatinase -
  DOU34_RS16760 speE 3372242..3373072 (-) 831 WP_003151052.1 spermidine synthase -

Sequence

Protein


Download         Length: 39 a.a.        Molecular weight: 4295.19 Da        Isoelectric Point: 10.0233

>NTDB_id=299150 DOU34_RS16715 WP_007407639.1 3368354..3368473(+) (phrF) [Bacillus amyloliquefaciens strain SH-B74]
MKLKYKLALTCLAISTVFVSAEIASAPKHQFEVAQRGMI

Nucleotide


Download         Length: 120 bp        

>NTDB_id=299150 DOU34_RS16715 WP_007407639.1 3368354..3368473(+) (phrF) [Bacillus amyloliquefaciens strain SH-B74]
ATGAAATTGAAGTATAAATTAGCACTTACTTGCTTGGCAATAAGCACTGTTTTTGTTTCAGCCGAAATCGCAAGTGCACC
GAAACATCAATTTGAAGTTGCTCAGCGCGGAATGATTTAA

Domains

Predicted by InterproScan.

Rap-phr extracellular signalling (PhrC_PhrF)

(3-39)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A3KLD5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  phrF Bacillus subtilis subsp. subtilis str. 168

74.074

69.231

0.513
  phrC Bacillus subtilis subsp. subtilis str. 168

46.154

100

0.462

Multiple sequence alignment