Detailed information    

insolico Bioinformatically predicted

Overview

Name   rapF   Type   Regulator
Locus tag   DOU34_RS16710 Genome accession   NZ_CP030097
Coordinates   3367225..3368370 (+) Length   381 a.a.
NCBI ID   WP_007407638.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain SH-B74     
Function   inhibit the DNA-binding function of ComA (predicted from homology)   
Competence regulation

Genomic Context

Location: 3362225..3373370
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DOU34_RS16685 - 3363122..3363550 (-) 429 WP_007407634.1 DUF1934 domain-containing protein -
  DOU34_RS16690 - 3363704..3364216 (+) 513 Protein_3251 bacteriocin biosynthesis protein AlbG -
  DOU34_RS16695 - 3364223..3365599 (-) 1377 WP_061047017.1 YncE family protein -
  DOU34_RS16700 - 3365639..3366994 (-) 1356 WP_061047016.1 YncE family protein -
  DOU34_RS16710 rapF 3367225..3368370 (+) 1146 WP_007407638.1 tetratricopeptide repeat protein Regulator
  DOU34_RS16715 phrF 3368354..3368473 (+) 120 WP_007407639.1 PhrC/PhrF family phosphatase-inhibitory pheromone Regulator
  DOU34_RS16720 - 3368671..3368874 (+) 204 WP_007407640.1 helix-turn-helix transcriptional regulator -
  DOU34_RS16725 - 3368888..3369091 (+) 204 WP_007407641.1 hypothetical protein -
  DOU34_RS16730 - 3369126..3369311 (+) 186 WP_061047015.1 DUF4177 domain-containing protein -
  DOU34_RS16735 - 3369497..3369778 (+) 282 WP_007407643.1 hypothetical protein -
  DOU34_RS16740 - 3369822..3370334 (+) 513 WP_061047014.1 stage II sporulation protein M -
  DOU34_RS16750 - 3370925..3371143 (-) 219 WP_003151056.1 LuxR C-terminal-related transcriptional regulator -
  DOU34_RS16755 speB 3371311..3372183 (-) 873 WP_007407646.1 agmatinase -
  DOU34_RS16760 speE 3372242..3373072 (-) 831 WP_003151052.1 spermidine synthase -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 45543.57 Da        Isoelectric Point: 4.5769

>NTDB_id=299149 DOU34_RS16710 WP_007407638.1 3367225..3368370(+) (rapF) [Bacillus amyloliquefaciens strain SH-B74]
MASVLSSSAVGEKINEWYMYIRRFSIPDAEYLRREIKEELAGLEDDQDLHLYYSLMEFRHNLMLEYLEPLESQRIEEQPR
LSDLLADIDKKQARLTGRLDYYFNFFRGMYELECREYLSAIQFFKKAEYKLDFVKDCIEKAEFYFKMSESYYYMKQTYFS
MDYARQAYKIYHKQEAYNIRVLQCHSLFATNFLDLKQYDEAIQHFKKAYAMAEAEQQPQLMGRTLYNIGLCFNSQENYKP
AIDYIKRAIAVFEDGNIITSLPQAYFLITQIHYKIGNMAIARQYHDKGVSYAEEAEDSLYIAEYEFLESLYVGEPDEEAI
MECFDFLRDKSMYADLEDFALDVAKYYHERENFEKASAYFLKVEETRQQIQGGVKLYEIEV

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=299149 DOU34_RS16710 WP_007407638.1 3367225..3368370(+) (rapF) [Bacillus amyloliquefaciens strain SH-B74]
GTGGCGAGTGTTTTATCATCTTCAGCGGTTGGAGAAAAAATCAACGAATGGTATATGTACATTCGAAGGTTCAGCATCCC
GGACGCCGAGTACTTGCGGAGAGAAATCAAAGAAGAACTTGCAGGTCTGGAAGATGATCAGGATCTTCATCTATACTATT
CTCTCATGGAATTCCGCCACAATCTGATGCTCGAATACCTCGAACCGCTGGAATCGCAGCGGATAGAAGAACAGCCGAGA
CTATCAGATCTGCTCGCGGATATTGATAAAAAGCAGGCCCGGCTGACCGGACGTCTTGATTATTATTTTAATTTTTTCCG
GGGGATGTATGAGCTGGAATGCCGTGAATATCTTTCAGCGATTCAGTTTTTTAAGAAAGCTGAATATAAGCTTGATTTTG
TCAAAGATTGTATCGAGAAAGCTGAATTTTATTTTAAGATGTCAGAGTCCTACTATTATATGAAACAGACTTATTTTTCT
ATGGATTATGCCCGGCAGGCTTATAAAATTTATCATAAGCAGGAGGCATATAATATAAGGGTGCTTCAGTGCCATTCATT
GTTCGCTACCAACTTTCTTGACTTAAAGCAATATGATGAAGCAATTCAGCATTTTAAAAAGGCTTATGCCATGGCAGAGG
CAGAACAGCAGCCACAATTAATGGGCCGGACTCTATATAATATCGGACTTTGTTTTAACAGCCAAGAAAATTACAAACCA
GCCATTGATTACATAAAACGGGCAATAGCTGTTTTTGAAGATGGGAACATCATCACTTCTCTCCCGCAAGCCTATTTCTT
AATCACACAAATACATTATAAAATCGGAAATATGGCCATAGCCAGACAATATCATGACAAAGGGGTATCTTATGCAGAGG
AGGCAGAAGATTCCCTATATATCGCCGAGTATGAATTTTTAGAATCTTTATATGTCGGTGAGCCTGATGAAGAAGCAATC
ATGGAATGTTTTGACTTTCTCAGAGATAAATCGATGTATGCTGATCTTGAAGATTTTGCCTTAGATGTGGCAAAATATTA
TCATGAAAGAGAAAATTTCGAGAAGGCTTCCGCATATTTTTTAAAGGTGGAAGAAACAAGACAGCAGATTCAAGGAGGTG
TGAAGTTGTATGAAATTGAAGTATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_12)

(189-253)

response regulator aspartate phosphatase H, N terminal (RapH_N)

(5-65)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rapF Bacillus subtilis subsp. subtilis str. 168

78.478

100

0.785
  rapC Bacillus subtilis subsp. subtilis str. 168

56.579

99.738

0.564

Multiple sequence alignment