Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   DOU34_RS15765 Genome accession   NZ_CP030097
Coordinates   3181393..3182778 (-) Length   461 a.a.
NCBI ID   WP_061047045.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain SH-B74     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3176393..3187778
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DOU34_RS15730 flgL 3176568..3177485 (-) 918 WP_003151396.1 flagellar hook-associated protein FlgL -
  DOU34_RS15735 flgK 3177497..3179014 (-) 1518 WP_014419008.1 flagellar hook-associated protein FlgK -
  DOU34_RS15740 - 3179030..3179512 (-) 483 WP_003151394.1 flagellar protein FlgN -
  DOU34_RS15745 flgM 3179527..3179793 (-) 267 WP_003151393.1 flagellar biosynthesis anti-sigma factor FlgM -
  DOU34_RS15750 - 3179863..3180282 (-) 420 WP_012118571.1 TIGR03826 family flagellar region protein -
  DOU34_RS15755 comFC 3180356..3181045 (-) 690 WP_196493397.1 phosphoribosyltransferase family protein Machinery gene
  DOU34_RS15760 - 3181051..3181335 (-) 285 WP_007407481.1 competence protein ComFB -
  DOU34_RS15765 comFA 3181393..3182778 (-) 1386 WP_061047045.1 DEAD/DEAH box helicase Machinery gene
  DOU34_RS15770 - 3182887..3183735 (-) 849 WP_061047044.1 DegV family protein -
  DOU34_RS15775 degU 3183833..3184522 (-) 690 WP_003219701.1 two-component system response regulator DegU Regulator
  DOU34_RS15780 degS 3184599..3185762 (-) 1164 WP_007407484.1 two-component sensor histidine kinase DegS Regulator
  DOU34_RS15785 - 3185985..3186632 (+) 648 WP_007407485.1 YigZ family protein -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52643.05 Da        Isoelectric Point: 10.2035

>NTDB_id=299143 DOU34_RS15765 WP_061047045.1 3181393..3182778(-) (comFA) [Bacillus amyloliquefaciens strain SH-B74]
MSEGASEFSEVRDFFYGRHLLRSEIPFSDQRIKRFTEKEYITAEPSIIRRKNRYVCQRCGQSDQACFAAFWAPSAKRQIT
YCRACVVMGRADELTSLYYWNQASENSWEPVKLSWEGTLTDGQKRAAAALTDAIKERQELLVWAVCGSGKTEMLFPGIEF
ALNHGLRVCVATPRTDVVLELLPRLKKAFEKVEVSALYGGSEDKGRLTPLMISTAHQLMRYRDIFDVMIIDEVDAFPFSA
DETLRFAVDKARKKNSALVYVTATPSDTLKKKAEAGLLKSVRIPARYHRKPLPEPRFLWCGNWKKKLQKGKLPRSVTDWV
RQKLQSQLPVFLFVPSVHVLKKTTDYFQKLNIRAEGVHAEDTFRKDKVKRFRDGRLDLLVTTTILERGVTVPKVQTCVLG
AEAPIFTESALVQIAGRTGRHYKHFSGDVVMFHFGITNGMKKAKKHIEHMNKLAQKSKLLD

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=299143 DOU34_RS15765 WP_061047045.1 3181393..3182778(-) (comFA) [Bacillus amyloliquefaciens strain SH-B74]
ATGTCCGAAGGTGCTTCAGAATTTTCAGAGGTGCGGGATTTCTTTTACGGCCGGCATTTATTGAGATCTGAGATACCGTT
TTCAGACCAGCGCATTAAGCGTTTTACAGAAAAAGAATATATAACGGCCGAGCCGTCAATCATCCGCCGGAAAAATCGTT
ACGTCTGCCAACGATGCGGTCAGTCTGATCAAGCCTGCTTTGCGGCATTTTGGGCGCCCTCTGCCAAAAGGCAGATTACC
TATTGCCGTGCCTGTGTCGTGATGGGAAGGGCTGATGAACTGACATCATTATATTATTGGAATCAAGCCTCTGAAAACAG
CTGGGAACCTGTAAAGCTTTCTTGGGAAGGAACACTGACAGACGGACAGAAGCGGGCGGCCGCCGCGCTTACAGATGCGA
TAAAAGAGAGACAAGAGCTGTTAGTATGGGCGGTTTGCGGATCGGGGAAAACAGAGATGCTGTTTCCGGGTATCGAGTTT
GCTCTGAATCACGGTTTGCGCGTCTGTGTCGCGACTCCGCGTACAGATGTCGTACTTGAACTGCTGCCGAGACTGAAAAA
AGCATTTGAGAAAGTCGAAGTTTCTGCTTTGTACGGCGGCAGCGAGGACAAAGGCCGTCTGACTCCGTTAATGATTTCAA
CCGCTCACCAGCTGATGCGCTACCGAGACATTTTTGATGTCATGATAATAGATGAAGTAGATGCGTTTCCTTTTTCAGCC
GATGAGACATTAAGATTCGCTGTTGATAAAGCGAGGAAGAAAAACAGCGCCCTTGTTTATGTAACGGCAACTCCTTCAGA
CACATTAAAAAAGAAGGCTGAAGCCGGTTTGCTGAAAAGCGTCCGCATACCGGCCAGATATCATCGGAAACCCCTCCCGG
AGCCGCGTTTTTTGTGGTGCGGTAATTGGAAGAAGAAGCTTCAGAAAGGAAAACTGCCGCGCTCTGTAACGGATTGGGTC
CGCCAAAAACTTCAATCACAGCTGCCGGTTTTCTTATTCGTTCCGTCTGTACACGTATTGAAAAAGACAACGGATTATTT
TCAGAAATTAAACATACGGGCAGAAGGCGTTCATGCGGAAGACACATTCAGAAAGGATAAAGTGAAACGGTTCAGAGACG
GCCGGCTTGACCTGCTGGTAACGACTACGATTTTAGAAAGAGGGGTTACCGTGCCCAAAGTTCAGACATGTGTCCTCGGC
GCAGAAGCACCTATCTTTACTGAGAGCGCGCTCGTACAGATCGCGGGCAGAACGGGAAGGCATTATAAACATTTCAGCGG
TGATGTCGTCATGTTTCATTTCGGAATTACAAACGGAATGAAAAAAGCAAAAAAACATATAGAACATATGAACAAATTGG
CACAAAAAAGTAAATTGTTGGACTAG

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(328-421)

Type III restriction enzyme, res subunit (ResIII)

(118-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

62.174

99.783

0.62

Multiple sequence alignment