Detailed information NTG0299145

Bioinformatically predicted

Overview

• Name: degS
• Type: Regulator
• Locus tag: DOU34_RS15780
• Genome accession: NZ_CP030097
• Coordinates: 3184599..3185762 (-)
• Length: 387 a.a.
• NCBI ID: WP_007407484.1
• Uniprot ID: A3KLC1
• Organism: Bacillus amyloliquefaciens strain SH-B74
• Function: phosphorylation of DegU (predicted from homology) Competence regulation

Genomic Context

Location: 3179599..3190762

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DOU34_RS15750	-	3179863..3180282 (-)	420	WP_012118571.1	TIGR03826 family flagellar region protein	-
DOU34_RS15755	comFC	3180356..3181045 (-)	690	WP_196493397.1	phosphoribosyltransferase family protein	Machinery gene
DOU34_RS15760	-	3181051..3181335 (-)	285	WP_007407481.1	competence protein ComFB	-
DOU34_RS15765	comFA	3181393..3182778 (-)	1386	WP_061047045.1	DEAD/DEAH box helicase	Machinery gene
DOU34_RS15770	-	3182887..3183735 (-)	849	WP_061047044.1	DegV family protein	-
DOU34_RS15775	degU	3183833..3184522 (-)	690	WP_003219701.1	two-component system response regulator DegU	Regulator
DOU34_RS15780	degS	3184599..3185762 (-)	1164	WP_007407484.1	two-component sensor histidine kinase DegS	Regulator
DOU34_RS15785	-	3185985..3186632 (+)	648	WP_007407485.1	YigZ family protein	-
DOU34_RS15790	-	3186637..3187857 (+)	1221	WP_039063727.1	LCP family protein	-
DOU34_RS15795	-	3187956..3189032 (-)	1077	WP_003151382.1	MraY family glycosyltransferase	-
DOU34_RS15800	-	3189165..3190361 (-)	1197	WP_061047042.1	glycosyltransferase	-

Sequence

Protein

• Length: 387 a.a.
• Molecular weight: 45014.78 Da
• Isoelectric Point: 6.8616

>NTDB_id=299145 DOU34_RS15780 WP_007407484.1 3184599..3185762(-) (degS) [Bacillus amyloliquefaciens strain SH-B74]
MNKSKMDSKVLDSILMKMLKTVDGSKDEVFQIGEQSRQQYEQLVEELKQIKQQVYEVIELGDKLEVHTRHARNRLSEVSR
NFHKFSEEEIRNAYEKAHKLQVELTMIQQREKQLRERRDDLERRLLGLQEIIERSESLVSQITVVLNYLNQDLRQVGLLL
ADAQAKQDFGLRIIEAQEEERKRVSREIHDGPAQMLANVMMRSELIERIFRDRGTEDGFQEIRNLRQNVRNALYEVRRII
YDLRPMALDDLGLIPTLRKYLYTTEEYNGKVKIHFQCIGDTENQRLAPQFEVALFRLAQEAVTNALKHSESEEITVKVEV
TADFVVLIIKDNGKGFDIKDAKQKKNKSFGLLGMKERVDLLEGTITIDSKIGLGTFIMIKVPLSLGL

Nucleotide

• Length: 1164 bp

>NTDB_id=299145 DOU34_RS15780 WP_007407484.1 3184599..3185762(-) (degS) [Bacillus amyloliquefaciens strain SH-B74]
ATGAATAAATCGAAGATGGACTCCAAAGTTTTGGATTCTATACTTATGAAAATGCTGAAAACCGTGGACGGGAGCAAGGA
CGAGGTTTTTCAAATCGGAGAGCAGTCGCGCCAGCAATATGAGCAGCTGGTTGAAGAACTCAAACAGATCAAACAGCAGG
TCTACGAAGTCATTGAGCTGGGCGACAAACTTGAAGTTCATACGCGGCACGCACGAAACCGCTTGTCTGAAGTAAGCAGA
AATTTCCATAAGTTCAGCGAAGAAGAAATTCGCAACGCCTATGAAAAGGCGCACAAACTTCAAGTGGAACTGACGATGAT
CCAACAGCGTGAAAAGCAGCTGCGGGAGCGCAGAGATGATTTAGAACGCCGGCTTTTGGGGCTTCAGGAAATCATTGAAC
GGTCAGAATCATTGGTCAGCCAAATAACCGTCGTTCTGAATTACCTGAATCAAGACCTTCGCCAAGTAGGGCTTCTTCTT
GCCGATGCGCAGGCTAAACAAGATTTCGGTTTAAGAATCATTGAAGCCCAAGAGGAAGAAAGAAAAAGAGTTTCCAGAGA
AATTCATGACGGTCCCGCGCAAATGCTGGCAAATGTCATGATGAGATCGGAATTAATTGAACGGATCTTCCGCGACCGCG
GCACGGAGGACGGATTTCAGGAAATCAGAAATCTGCGGCAGAATGTGCGGAATGCCCTTTACGAAGTCAGAAGGATTATA
TATGATTTAAGACCGATGGCATTGGACGATCTCGGGCTGATTCCGACACTGAGAAAATATCTTTATACAACTGAAGAATA
CAACGGAAAGGTGAAAATACATTTTCAGTGTATCGGAGACACAGAGAACCAAAGGCTTGCCCCGCAATTTGAAGTGGCGC
TTTTCCGTCTCGCTCAGGAAGCCGTCACAAATGCACTCAAGCATTCAGAATCAGAGGAAATTACAGTAAAAGTGGAAGTA
ACCGCTGATTTTGTCGTTCTCATCATTAAGGACAACGGAAAAGGCTTTGATATTAAGGATGCTAAACAAAAGAAAAACAA
ATCATTCGGCTTGCTGGGAATGAAAGAAAGAGTAGATCTGCTTGAAGGAACGATAACGATAGACTCTAAAATAGGTCTTG
GGACATTTATCATGATTAAGGTTCCGTTATCTCTCGGCCTATAA

Domains

Predicted by InterproScan.

• Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c) (291-384)

• Sensor protein DegS (DegS) (12-170)

• Histidine kinase (HisKA_3) (180-248)

3D structure

• AlphaFold DB: A3KLC1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
degS	Bacillus subtilis subsp. subtilis str. 168	95.584	99.483	0.951