Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   DOU34_RS11280 Genome accession   NZ_CP030097
Coordinates   2323741..2324355 (-) Length   204 a.a.
NCBI ID   WP_020956097.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain SH-B74     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2318741..2329355
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DOU34_RS11255 rpsT 2319044..2319310 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  DOU34_RS11260 holA 2319327..2320368 (-) 1042 Protein_2205 DNA polymerase III subunit delta -
  DOU34_RS20785 - 2320408..2320559 (-) 152 Protein_2206 hypothetical protein -
  DOU34_RS11265 - 2320600..2320734 (+) 135 WP_003152870.1 YqzM family protein -
  DOU34_RS11270 comEC 2320753..2323104 (-) 2352 WP_061046608.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  DOU34_RS11275 - 2323105..2323674 (-) 570 WP_003152868.1 ComE operon protein 2 -
  DOU34_RS11280 comEA 2323741..2324355 (-) 615 WP_020956097.1 helix-hairpin-helix domain-containing protein Machinery gene
  DOU34_RS11285 comER 2324414..2325235 (+) 822 WP_061046607.1 late competence protein ComER -
  DOU34_RS11290 - 2325304..2326041 (-) 738 WP_015417856.1 class I SAM-dependent methyltransferase -
  DOU34_RS11295 rsfS 2326038..2326394 (-) 357 WP_007408260.1 ribosome silencing factor -
  DOU34_RS11300 yqeK 2326412..2326972 (-) 561 WP_014418422.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  DOU34_RS11305 - 2326962..2327531 (-) 570 WP_007408258.1 nicotinate-nucleotide adenylyltransferase -
  DOU34_RS11310 yhbY 2327542..2327832 (-) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -
  DOU34_RS11315 aroE 2327826..2328668 (-) 843 WP_061046606.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22251.45 Da        Isoelectric Point: 6.4940

>NTDB_id=299128 DOU34_RS11280 WP_020956097.1 2323741..2324355(-) (comEA) [Bacillus amyloliquefaciens strain SH-B74]
MTKAMMTERLRRNLKILGACLVAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREEKGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=299128 DOU34_RS11280 WP_020956097.1 2323741..2324355(-) (comEA) [Bacillus amyloliquefaciens strain SH-B74]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGTAGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGACAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCTTACCGAGAAGAAAAAGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAGAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(68-122)

Helix-hairpin-helix motif (HHH_3)

(141-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.115

93.627

0.544
  comEA Latilactobacillus sakei subsp. sakei 23K

31.897

100

0.363
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

35.071

100

0.363

Multiple sequence alignment