Detailed information NTG0299126

Bioinformatically predicted

Overview

• Name: comEC
• Type: Machinery gene
• Locus tag: DOU34_RS11270
• Genome accession: NZ_CP030097
• Coordinates: 2320753..2323104 (-)
• Length: 783 a.a.
• NCBI ID: WP_061046608.1
• Uniprot ID: -
• Organism: Bacillus amyloliquefaciens strain SH-B74
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2315753..2328104

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DOU34_RS11240	-	2316118..2316456 (-)	339	WP_007408270.1	YqxA family protein	-
DOU34_RS11245	-	2316473..2317666 (-)	1194	WP_007612673.1	stage II sporulation protein P	-
DOU34_RS11250	gpr	2317735..2318841 (-)	1107	WP_007408268.1	GPR endopeptidase	-
DOU34_RS11255	rpsT	2319044..2319310 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
DOU34_RS11260	holA	2319327..2320368 (-)	1042	Protein_2205	DNA polymerase III subunit delta	-
DOU34_RS20785	-	2320408..2320559 (-)	152	Protein_2206	hypothetical protein	-
DOU34_RS11265	-	2320600..2320734 (+)	135	WP_003152870.1	YqzM family protein	-
DOU34_RS11270	comEC	2320753..2323104 (-)	2352	WP_061046608.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
DOU34_RS11275	-	2323105..2323674 (-)	570	WP_003152868.1	ComE operon protein 2	-
DOU34_RS11280	comEA	2323741..2324355 (-)	615	WP_020956097.1	helix-hairpin-helix domain-containing protein	Machinery gene
DOU34_RS11285	comER	2324414..2325235 (+)	822	WP_061046607.1	late competence protein ComER	-
DOU34_RS11290	-	2325304..2326041 (-)	738	WP_015417856.1	class I SAM-dependent methyltransferase	-
DOU34_RS11295	rsfS	2326038..2326394 (-)	357	WP_007408260.1	ribosome silencing factor	-
DOU34_RS11300	yqeK	2326412..2326972 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
DOU34_RS11305	-	2326962..2327531 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
DOU34_RS11310	yhbY	2327542..2327832 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-

Sequence

Protein

• Length: 783 a.a.
• Molecular weight: 86628.53 Da
• Isoelectric Point: 9.0082

>NTDB_id=299126 DOU34_RS11270 WP_061046608.1 2320753..2323104(-) (comEC) [Bacillus amyloliquefaciens strain SH-B74]
MKYKYLLLPLAAVSATAGIAAAHVFWVLLLFLLYLLFIIVKTKQHAPVIVCLVSFCLYFFLYTVCDAANVTRYQAGSYTE
QAVITNIPKVDGAKMSAVIRTHDKEKWAASYKIRSLEEKRLIEQLEPGMRCTFTGSLEQPAHATVPGGFDYKEYLYSQQI
HWLFSVTSIQQCEKSKQPLLKLLNIRKNLISIIRNHVPESSAGIVEALTLGERFSIEDDILSAYQNLGVVHLMAISGMHV
GLITAGLFYALIRIGLTREKAGILLLLFLPVYTLLSGAAPSVLRASLMLGFYIAGTLVKRGIHSSAALSLSYLLLLLFNP
YFLWQAGFQLSFAVSASLILSSSILKKAGKSRLAGLAMASLIAELSSLPFLLYHFQQISLVSFPMNMVMVPFYTLFVIPV
SVISFLLLLLSRQMGECLFDMFDLVMKPVHDFITYAASVDLFTMIVSKPDFLSLLLLAVSVFTLFAALEKGGFLKLRKSA
LFFCAILAYLICRPYFSPWGEADMLDIGQGDSLFISAPHRKGTVMVDTGGVIAYPGESWKEKRHPYSIGEKVLIPFLNGK
GVKKLDALILTHADQDHIGEAGVLIKNHRVKRLIVPVGFVKEPKDQNILNMAKENNIPVAEAKRGDTITAGDLQFQVLSP
ESSDGKSKNDSSLVLWTVLGGVSWLLTGDLESDGETEVLKTYPNLKADILKAGHHGSKSSTSEAFLKQLQPEAALISAGK
ENRYHHPHEEVLDRLKAYSVNVLRTDVSGTIQYRFKKGAGTFSVFPPYDIEETRAQEVKKTAD

Nucleotide

• Length: 2352 bp

>NTDB_id=299126 DOU34_RS11270 WP_061046608.1 2320753..2323104(-) (comEC) [Bacillus amyloliquefaciens strain SH-B74]
ATGAAATATAAATACCTTCTTCTGCCTCTGGCGGCGGTTTCTGCAACTGCGGGAATTGCCGCCGCTCATGTCTTCTGGGT
TCTGCTCCTTTTTCTTCTGTATCTTCTCTTTATTATTGTAAAAACAAAGCAGCATGCTCCGGTTATCGTCTGCCTCGTTT
CTTTTTGTCTTTATTTCTTTCTTTATACGGTTTGTGACGCTGCGAATGTAACGCGCTATCAGGCCGGCAGTTATACTGAA
CAGGCCGTCATCACTAATATTCCGAAGGTTGACGGAGCGAAAATGTCAGCCGTTATCCGTACACATGACAAGGAAAAATG
GGCGGCTTCGTACAAAATCCGGTCTCTTGAGGAAAAGAGACTCATTGAACAGCTTGAACCGGGGATGCGCTGCACGTTTA
CAGGCTCTCTGGAACAGCCTGCACATGCGACGGTTCCCGGAGGTTTTGATTATAAGGAATATCTTTACTCTCAGCAGATT
CACTGGTTATTTTCCGTAACTTCCATTCAGCAGTGTGAAAAATCCAAACAGCCGCTGTTGAAACTGCTGAACATCAGAAA
AAATTTGATTTCGATCATTAGGAATCACGTGCCTGAATCTTCCGCCGGAATTGTTGAAGCGCTGACCTTAGGTGAAAGAT
TTTCTATAGAGGACGATATACTGAGTGCATATCAAAATTTGGGAGTCGTTCATTTAATGGCGATTTCCGGAATGCATGTC
GGTCTTATTACGGCGGGATTATTTTATGCTCTGATCAGAATCGGGCTGACAAGAGAAAAAGCAGGAATTTTGCTGCTGTT
GTTTTTGCCGGTGTATACGCTGCTGAGCGGTGCCGCCCCATCTGTATTGCGCGCATCCCTCATGCTGGGATTTTATATCG
CCGGAACTCTTGTTAAACGCGGCATTCATTCCTCTGCTGCATTGTCCCTGTCTTATCTGCTGCTCCTGCTGTTTAATCCT
TACTTCCTTTGGCAGGCGGGCTTCCAGCTTTCCTTTGCGGTAAGCGCCTCTTTAATTCTGTCATCCTCCATTTTAAAGAA
AGCAGGGAAAAGCAGACTTGCTGGGCTTGCGATGGCTTCATTGATTGCAGAGCTCAGCTCACTTCCGTTTCTTCTCTATC
ATTTTCAGCAGATTTCACTTGTCAGTTTTCCGATGAATATGGTGATGGTGCCATTTTATACGTTATTTGTCATTCCGGTT
TCTGTCATCAGTTTCCTTCTTCTTTTACTTTCAAGGCAGATGGGAGAATGTTTGTTTGATATGTTCGACCTTGTGATGAA
GCCTGTACATGATTTCATTACATATGCGGCATCCGTTGATTTATTTACTATGATTGTGTCAAAGCCTGACTTTCTTTCCC
TTCTTCTGCTTGCGGTTTCCGTTTTTACGCTTTTTGCGGCTTTGGAAAAGGGAGGTTTTTTAAAACTCAGGAAATCGGCT
CTTTTTTTCTGCGCGATTTTGGCTTATTTAATATGCCGTCCGTATTTCAGTCCATGGGGAGAAGCGGATATGCTTGATAT
CGGGCAGGGAGACTCACTGTTTATAAGCGCGCCGCACCGCAAAGGGACCGTAATGGTTGATACAGGGGGAGTGATTGCTT
ATCCCGGAGAATCATGGAAAGAAAAACGCCACCCGTATTCTATCGGCGAGAAGGTTTTGATTCCGTTTTTAAACGGAAAA
GGGGTGAAAAAGCTGGATGCGCTGATTTTAACCCATGCGGATCAAGATCACATCGGAGAAGCCGGAGTGTTAATCAAAAA
TCATAGAGTCAAACGGTTAATTGTCCCCGTGGGATTTGTAAAAGAACCGAAGGATCAGAACATATTAAATATGGCGAAAG
AAAACAACATTCCCGTTGCCGAAGCAAAGCGGGGCGACACCATTACAGCCGGTGATCTTCAGTTTCAGGTGCTGTCTCCG
GAGTCGTCTGACGGAAAGAGTAAAAATGATTCGTCACTGGTGCTTTGGACGGTTTTAGGCGGAGTGAGCTGGCTTTTGAC
GGGAGATTTAGAATCGGACGGCGAAACGGAAGTGCTGAAAACGTATCCGAATCTGAAGGCTGATATATTGAAGGCGGGTC
ATCACGGCAGCAAAAGCTCTACGAGTGAAGCCTTTTTGAAGCAGCTTCAGCCGGAAGCAGCGCTGATTTCAGCAGGAAAA
GAGAATCGATACCATCATCCGCATGAAGAAGTGCTGGATCGTTTGAAGGCGTACTCTGTCAATGTGCTTCGCACCGATGT
CAGCGGAACGATTCAATACAGATTTAAAAAAGGCGCCGGAACGTTTTCCGTCTTCCCTCCATATGATATAGAAGAAACCA
GGGCGCAAGAAGTAAAAAAGACTGCCGATTGA

Domains

Predicted by InterproScan.

• Metallo-beta-lactamase superfamily (Lactamase_B) (508-719)

• Domain of unknown function (DUF4131) (DUF4131) (25-171)

• Competence protein (Competence) (208-469)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEC	Bacillus subtilis subsp. subtilis str. 168	56.995	98.595	0.562