Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   DOU34_RS10860 Genome accession   NZ_CP030097
Coordinates   2247240..2248310 (-) Length   356 a.a.
NCBI ID   WP_012117985.1    Uniprot ID   A0A411A7V3
Organism   Bacillus amyloliquefaciens strain SH-B74     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2242240..2253310
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DOU34_RS10810 - 2242300..2242884 (-) 585 WP_007408328.1 signal peptidase I -
  DOU34_RS10815 tapA 2242856..2243527 (-) 672 WP_020954230.1 amyloid fiber anchoring/assembly protein TapA -
  DOU34_RS10820 - 2243786..2244115 (+) 330 WP_020954231.1 DUF3889 domain-containing protein -
  DOU34_RS10825 - 2244155..2244334 (-) 180 WP_003153093.1 YqzE family protein -
  DOU34_RS10830 comGG 2244391..2244768 (-) 378 WP_032866434.1 competence type IV pilus minor pilin ComGG Machinery gene
  DOU34_RS10835 comGF 2244769..2245269 (-) 501 WP_257645080.1 competence type IV pilus minor pilin ComGF -
  DOU34_RS10840 comGE 2245178..2245492 (-) 315 WP_007408323.1 competence type IV pilus minor pilin ComGE -
  DOU34_RS10845 comGD 2245476..2245913 (-) 438 WP_007408322.1 competence type IV pilus minor pilin ComGD Machinery gene
  DOU34_RS10850 comGC 2245903..2246169 (-) 267 WP_042635730.1 competence type IV pilus major pilin ComGC Machinery gene
  DOU34_RS10855 comGB 2246216..2247253 (-) 1038 WP_061046620.1 competence type IV pilus assembly protein ComGB Machinery gene
  DOU34_RS10860 comGA 2247240..2248310 (-) 1071 WP_012117985.1 competence type IV pilus ATPase ComGA Machinery gene
  DOU34_RS10865 - 2248502..2249452 (-) 951 WP_015417820.1 magnesium transporter CorA family protein -
  DOU34_RS10870 - 2249598..2250899 (+) 1302 WP_020956081.1 hemolysin family protein -
  DOU34_RS10875 - 2250937..2251767 (-) 831 WP_015240214.1 STAS domain-containing protein -
  DOU34_RS10885 - 2251974..2252354 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  DOU34_RS10890 - 2252591..2252836 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40557.86 Da        Isoelectric Point: 9.4784

>NTDB_id=299124 DOU34_RS10860 WP_012117985.1 2247240..2248310(-) (comGA) [Bacillus amyloliquefaciens strain SH-B74]
MKNIETYSRNIINEAYTARASDIHIVPRERDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=299124 DOU34_RS10860 WP_012117985.1 2247240..2248310(-) (comGA) [Bacillus amyloliquefaciens strain SH-B74]
GTGAAAAATATAGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAGGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTAATTTCTCATTTTAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCCTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCCACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAAAGGCATTTCAACCGGCGTATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTCGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC
CATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A411A7V3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.303

100

0.823
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374

Multiple sequence alignment